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Image Search Results
Journal: Applied and Environmental Microbiology
Article Title: Molecular Determinants of the Thickened Matrix in a Dual-Species Pseudomonas aeruginosa and Enterococcus faecalis Biofilm
doi: 10.1128/AEM.01182-17
Figure Lengend Snippet: Polymicrobial biofilm tests of P. aeruginosa and E. faecalis. (A) Colony observation of cocultured P. aeruginosa and E. faecalis (red arrows). The growth experiment of P. aeruginosa (gray triangle) and E. faecalis (black square), grown individually (B) or together (C), in a shaking incubator at 37°C. The viable count assay was used to determine the CFU per milliliter. (D) The CV biofilm assay of the monospecies and the dual-species biofilms. *, P < 0.001 versus the biofilm levels of PAO1 and PAO1 plus E. faecalis (PAEF) or E. faecalis (EF). (E) Viscosity measurements of the mono- and dual-species biofilms. Viscosity appears in units of centipoise (cP). *, P < 0.0001 versus viscosity of PAO1 and PAEF or EF. (F) SEM images of the mono- and dual-species biofilms of P. aeruginosa and/or E. faecalis. The magnifications are ×5,000. (G) Images of the mono- and dual-species biofilms of P. aeruginosa and/or E. faecalis dripping out of the 96-well plates. Detailed video clips are provided in the movies in the supplemental material.
Article Snippet: PAO1 ,
Techniques: Biofilm Production Assay, Viscosity
Journal: Applied and Environmental Microbiology
Article Title: Molecular Determinants of the Thickened Matrix in a Dual-Species Pseudomonas aeruginosa and Enterococcus faecalis Biofilm
doi: 10.1128/AEM.01182-17
Figure Lengend Snippet: Extracellular DNA detection and DNase I treatment of mono- and dual-species P. aeruginosa and E. faecalis biofilms. The monospecies biofilms of E. faecalis (A), P. aeruginosa (B), and the dual-species biofilm of E. faecalis and P. aeruginosa (C) were stained for intracellular DNA (Syto60) and extracellular DNA (TOTO-1). Wavelengths of 652 nm and 514 nm were used for Syto60 and TOTO-1, respectively. (D) Crystal violet biofilm assay results when two different concentrations of DNase I (10 μg/ml and 100 μg/ml) were used to treat 32-h-old mature biofilms.
Article Snippet: PAO1 ,
Techniques: Staining, Biofilm Production Assay
Journal: Applied and Environmental Microbiology
Article Title: Molecular Determinants of the Thickened Matrix in a Dual-Species Pseudomonas aeruginosa and Enterococcus faecalis Biofilm
doi: 10.1128/AEM.01182-17
Figure Lengend Snippet: Scheme of the dual-species P. aeruginosa and E. faecalis biofilm development. (A) P. aeruginosa and E. faecalis attach to a surface without spatial separation. (B) During the early stages of dual-species biofilm formation, the attached cells proliferate and start producing EPS, Pel (blue straight line), and Psl (purple curved line). (C) P. aeruginosa and E. faecalis start to exhibit distinct spatial distributions. (D) In the maturation stage of the dual-species biofilm, P. aeruginosa forms a structured biofilm on top of the E. faecalis biofilm: Psl is associated with the surface and between P. aeruginosa, while Pel is associated with interspecies interactions between P. aeruginosa and E. faecalis.
Article Snippet: PAO1 ,
Techniques: