Journal: NPJ Schizophrenia

Article Title: Region-specific inhibition of 14-3-3 proteins induces psychomotor behaviors in mice

doi: 10.1038/s41537-018-0069-1

Figure Lengend Snippet: Behavioral deficits induced by YFP-difopein expression in WT mice. a Compared with WT mice injected with AAV-YFP in both the HP and PFC ( n = 14), bilateral injections of AAV-YFP-difopein to the HP alone significantly reduce freezing behavior in contextual fear conditioning test 24 h after training ( n = 6; F (1, 18) = 6.994; p = 0.016). However, injections of AAV-YFP-difopein to PFC alone does not affect freezing behavior when compared with WT controls ( n = 6; F (1, 18) = 1.505; p = 0.236). b Bilateral injections of AAV-YFP-difopein into the HP, either alone ( n = 8; F (1, 7.34) = 27.826; p = 0.001) or together with injection to the PFC ( n = 9; F (1, 9.668) = 17.64; p = 0.002), significantly increase travel distances in open field test, compared with WT mice injected with AAV-YFP (HP: n = 9, HP + PFC: n = 6). However, injections of AAV-YFP-difopein to the PFC alone ( n = 7; F (1, 10) = 1.953; p = 0.192) did not affect locomotive behavior of WT mice. c Bilateral injections of AAV-YFP-difopein to the HP of WT mice causes a decrease in pre-pulse inhibition (PPI) at all pre-pulse levels tested, while PPI is significantly lower at the pre-pulse level of 67 dB ( n = 5; F (1, 8) = 6.746; p = 0.032), compared with WT mice with AAV-YFP injected to the HP of ( n = 5). Data are presented as mean ± S.E.M. with statistical significance denoted as: * p < 0.05; ** p < 0.01; one-way ANOVA

Article Snippet: Startle response and pre-pulse inhibition (PPI) of the acoustic startle response were assessed using Acoustic Startle Reflex package (Med Associates).

Techniques: Expressing, Injection, Inhibition