Journal: PLoS ONE

Article Title: Proteomic fingerprinting in HIV/HCV co-infection reveals serum biomarkers for the diagnosis of fibrosis staging

doi: 10.1371/journal.pone.0195148

Figure Lengend Snippet: SELDI-TOF MS biomarkers detected in HIV/HCV co-infection.

Article Snippet: Samples were randomized within and across arrays with blank spots included as negative controls and applied to three types of ProteinChip arrays: weak cation exchange (CM10), immobilized metal affinity capture (IMAC30) and hydrophobic/reverse-phase (H50) (all from Bio-Rad).

Techniques:

Journal:

Article Title: Modern Tumor Marker Discovery in Urology: Surface Enhanced Laser Desorption and Ionization (SELDI)

doi:

Figure Lengend Snippet: (A) The SELDI process: The sample is added to the ProteinChip; after washing, only the retained proteins are subjected to laser desorption and ionization as depicted in panel B. (B) Schematic of SELDI ProteinChip Reader: The ProteinChip is inserted into the reader; a laser beam is focused on the sample, which causes the proteins embedded in the sample to desorb and ionize. The resulting ions are then accelerated through a vacuum tube to an ion detector. The ionized proteins are detected and the mass is determined based on their time-of-flight. EAM, energy absorbing molecule. Data from Ciphergen Biosystems (http://www.ciphergen.com).

Article Snippet: This technology is a type of affinity-based mass spectrometry 21 – 23 , 35 , 36 in which the protein sample is directly applied to a pretreated surface termed the “ProteinChip” (Ciphergen Biosystems ProteinChip?

Techniques:

Journal:

Article Title: Modern Tumor Marker Discovery in Urology: Surface Enhanced Laser Desorption and Ionization (SELDI)

doi:

Figure Lengend Snippet: The ProteinChip Array. A variety of arrays are available. Each consists of 8 to 16 spots comprised of a specific chemically or biochemically modified surface. Chemically modified surfaces are used to retain groups of proteins based on specific chemical properties such as hydrophobicity and charge. Biochemical surfaces are used to isolate a specific protein or class of proteins. IMAC, immobilized metal affinity capture. Data from Ciphergen Biosystems website (http://www.ciphergen.com).

Article Snippet: This technology is a type of affinity-based mass spectrometry 21 – 23 , 35 , 36 in which the protein sample is directly applied to a pretreated surface termed the “ProteinChip” (Ciphergen Biosystems ProteinChip?

Techniques: Modification