Journal: bioRxiv

Article Title: Lac-Phe mediates the anti-obesity effect of metformin

doi: 10.1101/2023.11.02.565321

Figure Lengend Snippet: (A) 300 mg/kg metformin treatment increases Lac-Phe levels in WT (n = 6), but not in CNDP2-KO (n = 7) mice. (B) Chronic metformin treatment (300 mg/kg daily) suppresses food intake of WT, but not CNDP2-KO mice. (C) Chronic metformin treatment (300 mg/kg daily) suppresses body weight of WT, but not CNDP2-KO mice. (D) 300 mg/kg metformin treatment increases circulating GDF15 levels in WT and CNDP2-KO mice. (E-F) 4 nmol subcutaneous GDF15 injection reduces food intake (E) and body weight (F) in WT and CNDP2-KO mice. Values adjusted by effects with vehicle treatment. (G) 300 mg/kg metformin treatment increases circulating GLP-1 levels in WT and CNDP2-KO mice. (H-I) 10 nmol subcutaneous Semaglutide injection reduces food intake (H) and body weight (I) in WT and CNDP2-KO mice. Values adjusted by effects with vehicle treatment. For (B) and (C), N = 6 for KO-saline, N = 7 for other groups. N = 4 – 5 in (D) and (G). N = 5 – 6 in (E), (F), (H), and (I). P values in (A) were calculated using multiple paired t tests with Holm-Šídák corrections; Welch t tests were used in (D-I).

Article Snippet: Recombinant GDF15 (957-GD) was purchased from R&D systems.

Techniques: Injection, Saline

Journal: bioRxiv

Article Title: Oncostatin M orchestrates collective epithelial migration via HIF1A activation

doi: 10.1101/2025.09.26.678830

Figure Lengend Snippet: (A) MCF10A cells were treated with EGF, OSM, or IFNG for 48 hours, then stained for DAPI (blue) and β-Catenin (red). (B) Cell velocity vectors represent speed and direction of cell motility, derived from cell tracking data during 24-48 hours after ligand treatment. Arrow size is proportional to velocity. (C-F) Quantification of cell phenotype from live-cell imaging. Shaded region represents 95% confidence interval from 3 biological replicates (n=3). Boxplots in (E) depict interquartile range of nearest neighbor distance calculated for each cell. (G-J) Line plots show protein expression levels normalized to the T0 control for each treatment condition. Error bars represent the full range of measurements (n=3).

Article Snippet: Afterward, cells were treated with either 10 ng/ml EGF (R&D Systems #236-EG), 10 ng/ml OSM (R&D Systems #8475-OM), or 20 ng/ml IFNG (R&D Systems #258-IF) + 10 ng/mL EGF.

Techniques: Staining, Derivative Assay, Cell Tracking Assay, Live Cell Imaging, Expressing, Control

Journal: bioRxiv

Article Title: Oncostatin M orchestrates collective epithelial migration via HIF1A activation

doi: 10.1101/2025.09.26.678830

Figure Lengend Snippet: (A) Workflow for the comparative network analysis to identify molecular subnetworks and nodes perturbed by OSM. Integrated molecular data collected from OSM and IFNG treated cells was analyzed using CausalPath. (B) Combined molecular network of OSM and IFNG activated nodes. The rewiring score between conditions is indicated by node color, and the top scoring nodes nominated for further investigation are indicated by increased size. (C) The top scoring nodes most rewired by OSM treatment, corresponding to the highlighted nodes in (B). (D) The majority (8/14) of top rewired nodes are centered around the STAT3 subnetwork.

Article Snippet: Afterward, cells were treated with either 10 ng/ml EGF (R&D Systems #236-EG), 10 ng/ml OSM (R&D Systems #8475-OM), or 20 ng/ml IFNG (R&D Systems #258-IF) + 10 ng/mL EGF.

Techniques:

Journal: PLoS ONE

Article Title: Innate Immune Responses after Airway Epithelial Stimulation with Mycobacterium bovis Bacille-Calmette Guérin

doi: 10.1371/journal.pone.0164431

Figure Lengend Snippet: (A) CXCL8 and (B) IL-6 production from epithelial cells stimulated with BCG in combination with IL-17A and/or IFN-γ for 0, 24, 48 and 72 hours, MOI 1:1. The 72 hours time points are in addition represented as bar graphs for (C) CXCL8 and (D) IL-6. The results are depicted as mean ± SD of three experiments. Means were compared with one way ANOVA followed by multiple comparisons test with Tukey’s correction and significance was accepted at *p < 0.05, **p< 0.01, or ***p < 0.001.

Article Snippet: For the cytokine stimulation experiments, recombinant IL-17A and/or IFNγ (10 ng/mL, 7955-IL, 285-IF, RnD Systems, Denmark) were added to the bottom insert 30 min before the addition of BCG.

Techniques:

Journal: PLoS ONE

Article Title: Innate Immune Responses after Airway Epithelial Stimulation with Mycobacterium bovis Bacille-Calmette Guérin

doi: 10.1371/journal.pone.0164431

Figure Lengend Snippet: Primary neutrophils were stimulated with BCG for one, two and three hours in the presence of IL-17A and/or IFN-γ. (A) NETs were studied in a light microscope after 2 hours and stained with DAPI and (B) large NET structures were observed in addition to single NETs. (C) Quantification of NETs was performed with Picogreen assay on cell supernatants after 3 hours and (D) over time at 1, 2 and 3 hours. Results are depicted as mean ± SD from a total of 4 donors. Means of samples were were compared with negative control using ANOVA followed by Dunnet’s multiple comparisons test and significance was accepted at *p < 0.05, **p< 0.01, or ***p < 0.001. Bars represent 25 μm (A, B left panel) or 100 μm (B, right panel)

Article Snippet: For the cytokine stimulation experiments, recombinant IL-17A and/or IFNγ (10 ng/mL, 7955-IL, 285-IF, RnD Systems, Denmark) were added to the bottom insert 30 min before the addition of BCG.

Techniques: Light Microscopy, Staining, Picogreen Assay, Negative Control