protein 5 fabp5 Search Results


93
Aviva Systems fabp5 elisa kit human
Comparison <t>of</t> <t>E-FABP</t> concentrations in tears and saliva between the wild-type (WT) mice and the NOD mice. ( A ) Tear E-FABP concentration in the NOD mice was significantly lower than in the WT mice. ( p = 0.0135). ( B ) Saliva E-FABP concentration in the NOD mice was also significantly lower than in the WT mice. ( p = 0.04). * represents p < 0.05. E-FABP: epidermal fatty acid binding protein.
Fabp5 Elisa Kit Human, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fabp5 elisa kit human/product/Aviva Systems
Average 93 stars, based on 1 article reviews
fabp5 elisa kit human - by Bioz Stars, 2025-02
93/100 stars
  Buy from Supplier

93
Proteintech fabp5
Comparison <t>of</t> <t>E-FABP</t> concentrations in tears and saliva between the wild-type (WT) mice and the NOD mice. ( A ) Tear E-FABP concentration in the NOD mice was significantly lower than in the WT mice. ( p = 0.0135). ( B ) Saliva E-FABP concentration in the NOD mice was also significantly lower than in the WT mice. ( p = 0.04). * represents p < 0.05. E-FABP: epidermal fatty acid binding protein.
Fabp5, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fabp5/product/Proteintech
Average 93 stars, based on 1 article reviews
fabp5 - by Bioz Stars, 2025-02
93/100 stars
  Buy from Supplier

93
Proteintech 12348 1 ap
Comparison <t>of</t> <t>E-FABP</t> concentrations in tears and saliva between the wild-type (WT) mice and the NOD mice. ( A ) Tear E-FABP concentration in the NOD mice was significantly lower than in the WT mice. ( p = 0.0135). ( B ) Saliva E-FABP concentration in the NOD mice was also significantly lower than in the WT mice. ( p = 0.04). * represents p < 0.05. E-FABP: epidermal fatty acid binding protein.
12348 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/12348 1 ap/product/Proteintech
Average 93 stars, based on 1 article reviews
12348 1 ap - by Bioz Stars, 2025-02
93/100 stars
  Buy from Supplier

93
Proteintech rabbit anti fabp5 human
Comparison <t>of</t> <t>E-FABP</t> concentrations in tears and saliva between the wild-type (WT) mice and the NOD mice. ( A ) Tear E-FABP concentration in the NOD mice was significantly lower than in the WT mice. ( p = 0.0135). ( B ) Saliva E-FABP concentration in the NOD mice was also significantly lower than in the WT mice. ( p = 0.04). * represents p < 0.05. E-FABP: epidermal fatty acid binding protein.
Rabbit Anti Fabp5 Human, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti fabp5 human/product/Proteintech
Average 93 stars, based on 1 article reviews
rabbit anti fabp5 human - by Bioz Stars, 2025-02
93/100 stars
  Buy from Supplier

93
Proteintech rabbit anti human fabp5 primary antibody
<t>FABP5</t> expression was upregulated in HCC. a, expression of FABP5 in HCC samples (N = 369) and normal samples (N = 160) from the TCGA data set. b, relative expression of FABP5 mRNA in HCC tissues compared with adjacent normal tissues were detected by RT-qPCR. c, relative expression of FABP5 protein in HCC tissues and adjacent normal tissues were detected by western blotting. Representative images of FABP5 expression in 48 paired HCC tissues(t) and adjacent normal tissues (n). d, the expression of FABP5 was negatively correlated with overall survival time in HCC patients from the TCGA data set. * P < .05 and *** P < .001 as compared with the vehicle control.
Rabbit Anti Human Fabp5 Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti human fabp5 primary antibody/product/Proteintech
Average 93 stars, based on 1 article reviews
rabbit anti human fabp5 primary antibody - by Bioz Stars, 2025-02
93/100 stars
  Buy from Supplier

Image Search Results


Comparison of E-FABP concentrations in tears and saliva between the wild-type (WT) mice and the NOD mice. ( A ) Tear E-FABP concentration in the NOD mice was significantly lower than in the WT mice. ( p = 0.0135). ( B ) Saliva E-FABP concentration in the NOD mice was also significantly lower than in the WT mice. ( p = 0.04). * represents p < 0.05. E-FABP: epidermal fatty acid binding protein.

Journal: International Journal of Molecular Sciences

Article Title: Salivary and Lacrimal Gland Alterations of the Epidermal Fatty Acid-Binding Protein (E-FABP) in Non-Obese Diabetic Mice

doi: 10.3390/ijms23073491

Figure Lengend Snippet: Comparison of E-FABP concentrations in tears and saliva between the wild-type (WT) mice and the NOD mice. ( A ) Tear E-FABP concentration in the NOD mice was significantly lower than in the WT mice. ( p = 0.0135). ( B ) Saliva E-FABP concentration in the NOD mice was also significantly lower than in the WT mice. ( p = 0.04). * represents p < 0.05. E-FABP: epidermal fatty acid binding protein.

Article Snippet: The E-FABP concentration in the resulting supernatants was measured using the FABP5 ELISA Kit (Human) (Aviva Systems Biology, San Diego, CA, USA) based on the standard sandwich ELISA technique and following the manufacturer’s instructions.

Techniques: Concentration Assay, Binding Assay

Comparison of E-FABP immunohistochemistry in the salivary gland between the wild-type (WT) mice and the NOD mice. Representative immunohistochemistry images showed less intense immunostaining in the acinar epithelium of the NOD mice ( B ) when compared with the WT mice ( A ) (yellow arrows). IHC: immunohistochemistry.

Journal: International Journal of Molecular Sciences

Article Title: Salivary and Lacrimal Gland Alterations of the Epidermal Fatty Acid-Binding Protein (E-FABP) in Non-Obese Diabetic Mice

doi: 10.3390/ijms23073491

Figure Lengend Snippet: Comparison of E-FABP immunohistochemistry in the salivary gland between the wild-type (WT) mice and the NOD mice. Representative immunohistochemistry images showed less intense immunostaining in the acinar epithelium of the NOD mice ( B ) when compared with the WT mice ( A ) (yellow arrows). IHC: immunohistochemistry.

Article Snippet: The E-FABP concentration in the resulting supernatants was measured using the FABP5 ELISA Kit (Human) (Aviva Systems Biology, San Diego, CA, USA) based on the standard sandwich ELISA technique and following the manufacturer’s instructions.

Techniques: Immunohistochemistry, Immunostaining

Comparison of E-FABP immunohistochemistry in the lacrimal glands of the wild-type (WT) mice and the NOD mice. Representative immunohistochemistry images showed increased immunostaining of the acinar epithelium in the NOD mice ( B , D ) compared to the WT mice ( A , C ) (yellow arrows). Moreover, a denser infiltration of inflammatory cells around the acinar units of the NOD mice compared to the WT mice can be observed (green arrow). IHC: immunohistochemistry.

Journal: International Journal of Molecular Sciences

Article Title: Salivary and Lacrimal Gland Alterations of the Epidermal Fatty Acid-Binding Protein (E-FABP) in Non-Obese Diabetic Mice

doi: 10.3390/ijms23073491

Figure Lengend Snippet: Comparison of E-FABP immunohistochemistry in the lacrimal glands of the wild-type (WT) mice and the NOD mice. Representative immunohistochemistry images showed increased immunostaining of the acinar epithelium in the NOD mice ( B , D ) compared to the WT mice ( A , C ) (yellow arrows). Moreover, a denser infiltration of inflammatory cells around the acinar units of the NOD mice compared to the WT mice can be observed (green arrow). IHC: immunohistochemistry.

Article Snippet: The E-FABP concentration in the resulting supernatants was measured using the FABP5 ELISA Kit (Human) (Aviva Systems Biology, San Diego, CA, USA) based on the standard sandwich ELISA technique and following the manufacturer’s instructions.

Techniques: Immunohistochemistry, Immunostaining

Comparison of E-FABP mRNA expressions in the salivary and lacrimal glands of the wild-type (WT) and the NOD mice. ( A ) In the salivary glands, the E-FABP mRNA expression in the NOD mice was significantly lower than that in the WT mice ( p = 0.001). ( B ) In the lacrimal glands, the E-FABP mRNA expression in the NOD mice was significantly higher than that in the WT mice ( p = 0.0008). * and ** represent p < 0.05 and p < 0.001, respectively. SG: salivary gland, LG: lacrimal gland, GAPDH: glyceraldehyde phosphate dehydrogenase.

Journal: International Journal of Molecular Sciences

Article Title: Salivary and Lacrimal Gland Alterations of the Epidermal Fatty Acid-Binding Protein (E-FABP) in Non-Obese Diabetic Mice

doi: 10.3390/ijms23073491

Figure Lengend Snippet: Comparison of E-FABP mRNA expressions in the salivary and lacrimal glands of the wild-type (WT) and the NOD mice. ( A ) In the salivary glands, the E-FABP mRNA expression in the NOD mice was significantly lower than that in the WT mice ( p = 0.001). ( B ) In the lacrimal glands, the E-FABP mRNA expression in the NOD mice was significantly higher than that in the WT mice ( p = 0.0008). * and ** represent p < 0.05 and p < 0.001, respectively. SG: salivary gland, LG: lacrimal gland, GAPDH: glyceraldehyde phosphate dehydrogenase.

Article Snippet: The E-FABP concentration in the resulting supernatants was measured using the FABP5 ELISA Kit (Human) (Aviva Systems Biology, San Diego, CA, USA) based on the standard sandwich ELISA technique and following the manufacturer’s instructions.

Techniques: Expressing

FABP5 expression was upregulated in HCC. a, expression of FABP5 in HCC samples (N = 369) and normal samples (N = 160) from the TCGA data set. b, relative expression of FABP5 mRNA in HCC tissues compared with adjacent normal tissues were detected by RT-qPCR. c, relative expression of FABP5 protein in HCC tissues and adjacent normal tissues were detected by western blotting. Representative images of FABP5 expression in 48 paired HCC tissues(t) and adjacent normal tissues (n). d, the expression of FABP5 was negatively correlated with overall survival time in HCC patients from the TCGA data set. * P < .05 and *** P < .001 as compared with the vehicle control.

Journal: Cancer Biology & Therapy

Article Title: Fatty acid binding protein 5 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by degradation of Krüppel-like factor 9 mediated by miR-889-5p via cAMP-response element binding protein

doi: 10.1080/15384047.2022.2094670

Figure Lengend Snippet: FABP5 expression was upregulated in HCC. a, expression of FABP5 in HCC samples (N = 369) and normal samples (N = 160) from the TCGA data set. b, relative expression of FABP5 mRNA in HCC tissues compared with adjacent normal tissues were detected by RT-qPCR. c, relative expression of FABP5 protein in HCC tissues and adjacent normal tissues were detected by western blotting. Representative images of FABP5 expression in 48 paired HCC tissues(t) and adjacent normal tissues (n). d, the expression of FABP5 was negatively correlated with overall survival time in HCC patients from the TCGA data set. * P < .05 and *** P < .001 as compared with the vehicle control.

Article Snippet: The sections were incubated at 4°C overnight with rabbit anti-human FABP5 primary antibody (Proteintech, cat. no. 12348-1-AP) and then with goat anti-rabbit secondary antibody (Proteintech, cat. no. PR30009) for 50 min at room temperature.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Correlation between  FABP5  expression and clinicopathological characteristics

Journal: Cancer Biology & Therapy

Article Title: Fatty acid binding protein 5 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by degradation of Krüppel-like factor 9 mediated by miR-889-5p via cAMP-response element binding protein

doi: 10.1080/15384047.2022.2094670

Figure Lengend Snippet: Correlation between FABP5 expression and clinicopathological characteristics

Article Snippet: The sections were incubated at 4°C overnight with rabbit anti-human FABP5 primary antibody (Proteintech, cat. no. 12348-1-AP) and then with goat anti-rabbit secondary antibody (Proteintech, cat. no. PR30009) for 50 min at room temperature.

Techniques: Expressing

FABP5 knockdown inhibited cell growth, migration and invasion in HCC cells. a, the mRNA and protein expression levels of FABP5 were analyzed in FABP5-knockdown HepG2 and SK-Hep-1 cells by RT-qPCR and western blotting. b, the growth curve of HCC cells was determined by CCK-8 assay. c, PI fluorescence pattern was applied for cell-cycle distribution in HCC cells transfected with shFABP5 or shNC. d, HCC cells transfected with shFABP5 or shNC were stained with Annexin-V FITC and PI to be measured apoptosis. Apoptotic cells are presented in the right-lower (Q3, early apoptosis) and right-upper (Q2, late apoptosis) quadrants of the plots. e, transwell assay revealed the migration abilities of HCC cells transfected with shFABP5 or shNC. f, transwell assay revealed the invasive abilities of HCC cells transfected with shFABP5 or shNC. Results are presented as mean ± S.E.M. (N = 3). Results were averaged from three independent experiments and presented as percentage of control levels. * p < .05, ** p < .01 and *** p < .001 as compared with the vehicle control. FITC, fluorescein isothiocyanate; PI, propidium iodide.

Journal: Cancer Biology & Therapy

Article Title: Fatty acid binding protein 5 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by degradation of Krüppel-like factor 9 mediated by miR-889-5p via cAMP-response element binding protein

doi: 10.1080/15384047.2022.2094670

Figure Lengend Snippet: FABP5 knockdown inhibited cell growth, migration and invasion in HCC cells. a, the mRNA and protein expression levels of FABP5 were analyzed in FABP5-knockdown HepG2 and SK-Hep-1 cells by RT-qPCR and western blotting. b, the growth curve of HCC cells was determined by CCK-8 assay. c, PI fluorescence pattern was applied for cell-cycle distribution in HCC cells transfected with shFABP5 or shNC. d, HCC cells transfected with shFABP5 or shNC were stained with Annexin-V FITC and PI to be measured apoptosis. Apoptotic cells are presented in the right-lower (Q3, early apoptosis) and right-upper (Q2, late apoptosis) quadrants of the plots. e, transwell assay revealed the migration abilities of HCC cells transfected with shFABP5 or shNC. f, transwell assay revealed the invasive abilities of HCC cells transfected with shFABP5 or shNC. Results are presented as mean ± S.E.M. (N = 3). Results were averaged from three independent experiments and presented as percentage of control levels. * p < .05, ** p < .01 and *** p < .001 as compared with the vehicle control. FITC, fluorescein isothiocyanate; PI, propidium iodide.

Article Snippet: The sections were incubated at 4°C overnight with rabbit anti-human FABP5 primary antibody (Proteintech, cat. no. 12348-1-AP) and then with goat anti-rabbit secondary antibody (Proteintech, cat. no. PR30009) for 50 min at room temperature.

Techniques: Migration, Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Fluorescence, Transfection, Staining, Transwell Assay

Decreased expression of FABP5 inhibited the growth of HCC cells in vivo. a, HepG2 cells and HepG2 cells stably transfected with shFABP5 RNA or shNC were implanted into nude mice by subcutaneous injection. Tumor volume was measured each 7 days by a calliper until the end of the experiment. The tumor growth curve was drawn. b, the mice and the tumors of different groups on the 28th day after injection. c, the weight of each xenograft tumor was measured at the end of the experiment. d, the differences in FABP5 mRNA levels between the shFABP5 group and the control groups were detected by RT-qPCR. e, the protein levels of FABP5 in the shFABP5 group and the control groups were measured by western blotting. f, the tumors were analyzed by immunohistochemical staining with anti-FABP5. Original magnification: 400 × . *** p < .001 as compared with the vehicle control.

Journal: Cancer Biology & Therapy

Article Title: Fatty acid binding protein 5 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by degradation of Krüppel-like factor 9 mediated by miR-889-5p via cAMP-response element binding protein

doi: 10.1080/15384047.2022.2094670

Figure Lengend Snippet: Decreased expression of FABP5 inhibited the growth of HCC cells in vivo. a, HepG2 cells and HepG2 cells stably transfected with shFABP5 RNA or shNC were implanted into nude mice by subcutaneous injection. Tumor volume was measured each 7 days by a calliper until the end of the experiment. The tumor growth curve was drawn. b, the mice and the tumors of different groups on the 28th day after injection. c, the weight of each xenograft tumor was measured at the end of the experiment. d, the differences in FABP5 mRNA levels between the shFABP5 group and the control groups were detected by RT-qPCR. e, the protein levels of FABP5 in the shFABP5 group and the control groups were measured by western blotting. f, the tumors were analyzed by immunohistochemical staining with anti-FABP5. Original magnification: 400 × . *** p < .001 as compared with the vehicle control.

Article Snippet: The sections were incubated at 4°C overnight with rabbit anti-human FABP5 primary antibody (Proteintech, cat. no. 12348-1-AP) and then with goat anti-rabbit secondary antibody (Proteintech, cat. no. PR30009) for 50 min at room temperature.

Techniques: Expressing, In Vivo, Stable Transfection, Transfection, Injection, Quantitative RT-PCR, Western Blot, Immunohistochemical staining, Staining

The expression of KLF9 was negatively correlated with FABP5. a, gene microarray analysis were applied to examine the differential expression genes in SK-Hep-1 cells before and after FABP5 knockdown (FC > |2|; P < .01). b, gene ontology (GO) enrichment analysis of differential expression genes in accordance with the biological processes, cellular component, and molecular function. c, KLF9 mRNA expression in HepG2 and SK-Hep-1 cells with FABP5 knockdown determined by RT-qPCR. d, the KLF9 expression in HCC tissue and adjacent tissues were detected by RT-qPCR. e, relative expression of KLF9 protein in HCC tissues and adjacent normal tissues were detected by Western blotting. Representative images of KLF9 expression in 48 paired HCC tissues (t) and adjacent normal tissues (n). e, the expression of KLF9 was positively correlated with overall survival time in HCC patients from the TCGA data set. F, A negative correlation was found between the protein expression level of FABP5 and KLF9 in HCC samples. *p < .05, **p < .01, and ***p < .001 as compared with the vehicle control.

Journal: Cancer Biology & Therapy

Article Title: Fatty acid binding protein 5 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by degradation of Krüppel-like factor 9 mediated by miR-889-5p via cAMP-response element binding protein

doi: 10.1080/15384047.2022.2094670

Figure Lengend Snippet: The expression of KLF9 was negatively correlated with FABP5. a, gene microarray analysis were applied to examine the differential expression genes in SK-Hep-1 cells before and after FABP5 knockdown (FC > |2|; P < .01). b, gene ontology (GO) enrichment analysis of differential expression genes in accordance with the biological processes, cellular component, and molecular function. c, KLF9 mRNA expression in HepG2 and SK-Hep-1 cells with FABP5 knockdown determined by RT-qPCR. d, the KLF9 expression in HCC tissue and adjacent tissues were detected by RT-qPCR. e, relative expression of KLF9 protein in HCC tissues and adjacent normal tissues were detected by Western blotting. Representative images of KLF9 expression in 48 paired HCC tissues (t) and adjacent normal tissues (n). e, the expression of KLF9 was positively correlated with overall survival time in HCC patients from the TCGA data set. F, A negative correlation was found between the protein expression level of FABP5 and KLF9 in HCC samples. *p < .05, **p < .01, and ***p < .001 as compared with the vehicle control.

Article Snippet: The sections were incubated at 4°C overnight with rabbit anti-human FABP5 primary antibody (Proteintech, cat. no. 12348-1-AP) and then with goat anti-rabbit secondary antibody (Proteintech, cat. no. PR30009) for 50 min at room temperature.

Techniques: Expressing, Microarray, Quantitative RT-PCR, Western Blot

KLF9 knockdown reversed the phenotypes caused by FABP5 knockdown in HCC cells. a-b, HepG2 and SK-Hep-1 cells upon co-transfection of shFABP5 with shKLF9 showed significantly reduced KLF9 expression at the mRNA and protein levels. c, CCK-8 assay showed that shKLF9 significantly reversed the inhibition of proliferation induced by FABP5 knockdown in HCC cells. d, transwell migration assay revealed that shKLF9 significantly rescued the migratory inhibition induced FABP5 knockdown in HCC cells. e, transwell invasion assay revealed that shKLF9 significantly rescued the inhibition of invasion induced by FABP5 knockdown in HCC cells. F, KLF9 knockdown reversed the cell cycle arrest caused by FABP5 knockdown in HCC cells. PI fluorescence pattern was applied for cell-cycle distribution. G, KLF9 knockdown reversed the apoptosis caused by FABP5 knockdown in HCC cells. HepG2 and SK-Hep-1 cells were stained with Annexin-V FITC and PI to be measured apoptosis. Apoptotic cells are presented in the right-lower (Q3, early apoptosis) and right-upper (Q2, late apoptosis) quadrants of the plots. Results are presented as mean ± S.E.M. (N = 3). * p < .05, ** p < .01, and ***p < .001 as compared with the vehicle control. Results were averaged from three independent experiments and presented as percentage of control levels. FITC, fluorescein isothiocyanate; PI, propidium iodide.

Journal: Cancer Biology & Therapy

Article Title: Fatty acid binding protein 5 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by degradation of Krüppel-like factor 9 mediated by miR-889-5p via cAMP-response element binding protein

doi: 10.1080/15384047.2022.2094670

Figure Lengend Snippet: KLF9 knockdown reversed the phenotypes caused by FABP5 knockdown in HCC cells. a-b, HepG2 and SK-Hep-1 cells upon co-transfection of shFABP5 with shKLF9 showed significantly reduced KLF9 expression at the mRNA and protein levels. c, CCK-8 assay showed that shKLF9 significantly reversed the inhibition of proliferation induced by FABP5 knockdown in HCC cells. d, transwell migration assay revealed that shKLF9 significantly rescued the migratory inhibition induced FABP5 knockdown in HCC cells. e, transwell invasion assay revealed that shKLF9 significantly rescued the inhibition of invasion induced by FABP5 knockdown in HCC cells. F, KLF9 knockdown reversed the cell cycle arrest caused by FABP5 knockdown in HCC cells. PI fluorescence pattern was applied for cell-cycle distribution. G, KLF9 knockdown reversed the apoptosis caused by FABP5 knockdown in HCC cells. HepG2 and SK-Hep-1 cells were stained with Annexin-V FITC and PI to be measured apoptosis. Apoptotic cells are presented in the right-lower (Q3, early apoptosis) and right-upper (Q2, late apoptosis) quadrants of the plots. Results are presented as mean ± S.E.M. (N = 3). * p < .05, ** p < .01, and ***p < .001 as compared with the vehicle control. Results were averaged from three independent experiments and presented as percentage of control levels. FITC, fluorescein isothiocyanate; PI, propidium iodide.

Article Snippet: The sections were incubated at 4°C overnight with rabbit anti-human FABP5 primary antibody (Proteintech, cat. no. 12348-1-AP) and then with goat anti-rabbit secondary antibody (Proteintech, cat. no. PR30009) for 50 min at room temperature.

Techniques: Cotransfection, Expressing, CCK-8 Assay, Inhibition, Transwell Migration Assay, Transwell Invasion Assay, Fluorescence, Staining

PI3K/AKT signaling pathway was involved in FABP5-induced KLF9 downregulation. a, KEGG pathway enrichment analysis of top 20 differentially expressed signaling pathways in FABP5 knockdown group compared with control group in SK-Hep-1 cells. b, PI3K/AKT signaling pathway was analyzed by western blotting in the indicated groups.

Journal: Cancer Biology & Therapy

Article Title: Fatty acid binding protein 5 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by degradation of Krüppel-like factor 9 mediated by miR-889-5p via cAMP-response element binding protein

doi: 10.1080/15384047.2022.2094670

Figure Lengend Snippet: PI3K/AKT signaling pathway was involved in FABP5-induced KLF9 downregulation. a, KEGG pathway enrichment analysis of top 20 differentially expressed signaling pathways in FABP5 knockdown group compared with control group in SK-Hep-1 cells. b, PI3K/AKT signaling pathway was analyzed by western blotting in the indicated groups.

Article Snippet: The sections were incubated at 4°C overnight with rabbit anti-human FABP5 primary antibody (Proteintech, cat. no. 12348-1-AP) and then with goat anti-rabbit secondary antibody (Proteintech, cat. no. PR30009) for 50 min at room temperature.

Techniques: Western Blot

miR-889-5p suppressed KLF9 expression by directly targeted KLF9. a, the expression of miR-889-5p was significantly decreased by FABP5 knockdown in HCC cells. b, bioinformatics analysis predicted that the 3ʹUTR sequence of KLF9 is complementary to the seed sequence of miR-889-5p.The core binding sequences of KLF9 were mutated (in red text). c, dual-luciferase reporter assay was performed to verify that miR-889-5p directly bound to the 3’-UTR sequences of KLF9 in the cells co-transfected with miR-889 mimics or NC with pGL3-KLF9-WT or pGL3-KLF9-Mut. d, relative expression of miR-889-5p in 48 HCC tissues compared with adjacent normal tissues was detected by RT-qPCR. E, miR-889-5p expression was negatively correlated with KLF9 expression and positively correlated with FABP5 expression in HCC tissues. f-g, increased expression of miR-889-5p attenuated KLF9 mRNA and protein expression of in HCC cells with shFABP5 treatment. *p < .05, **p < .01, and ***p < .001 as compared with the vehicle control.

Journal: Cancer Biology & Therapy

Article Title: Fatty acid binding protein 5 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by degradation of Krüppel-like factor 9 mediated by miR-889-5p via cAMP-response element binding protein

doi: 10.1080/15384047.2022.2094670

Figure Lengend Snippet: miR-889-5p suppressed KLF9 expression by directly targeted KLF9. a, the expression of miR-889-5p was significantly decreased by FABP5 knockdown in HCC cells. b, bioinformatics analysis predicted that the 3ʹUTR sequence of KLF9 is complementary to the seed sequence of miR-889-5p.The core binding sequences of KLF9 were mutated (in red text). c, dual-luciferase reporter assay was performed to verify that miR-889-5p directly bound to the 3’-UTR sequences of KLF9 in the cells co-transfected with miR-889 mimics or NC with pGL3-KLF9-WT or pGL3-KLF9-Mut. d, relative expression of miR-889-5p in 48 HCC tissues compared with adjacent normal tissues was detected by RT-qPCR. E, miR-889-5p expression was negatively correlated with KLF9 expression and positively correlated with FABP5 expression in HCC tissues. f-g, increased expression of miR-889-5p attenuated KLF9 mRNA and protein expression of in HCC cells with shFABP5 treatment. *p < .05, **p < .01, and ***p < .001 as compared with the vehicle control.

Article Snippet: The sections were incubated at 4°C overnight with rabbit anti-human FABP5 primary antibody (Proteintech, cat. no. 12348-1-AP) and then with goat anti-rabbit secondary antibody (Proteintech, cat. no. PR30009) for 50 min at room temperature.

Techniques: Expressing, Sequencing, Binding Assay, Luciferase, Reporter Assay, Transfection, Quantitative RT-PCR

FABP5 inhibits the expression of miR-889-5p by regulating CREB protein phosphorylation. a, the siRNA of NF-κB, c-Myc and CREB were transiently transferred into HepG2 and SK-Hep-1 cells. The expression of miR-889-5p was detected by RT-qPCR. b, the designed mutative model of miR-889-5p promoter. c, a luciferase reporter promoter system was employed to confirm CREB bind to miR-889-5p promoter. d, the expression of total and phosphorylated CREB protein were detected by western blotting in the indicated groups. e, CREB inhibitor KG-501 reverse the effect of FABP5 overexpression on miR-889-5p expression in HCC cells. **p < .01 and ***p < .001 as compared with the vehicle control. e, the scheme of the mechanism by which FABP5 affects HCC tumorigenesis. FABP5 improves CREB protein phosphorylation to upregulate the miR-889-5p expression by CREB binding to the miR-889-5p promoter region, whereby leading to downregulation of KLF9 by miR-889-5p binding to the 3ʹ-UTR of the KLF9 mRNA, potentiating the PI3K/AKT signaling pathway and promoting the proliferation, migration, and invasion of HCC cells.

Journal: Cancer Biology & Therapy

Article Title: Fatty acid binding protein 5 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by degradation of Krüppel-like factor 9 mediated by miR-889-5p via cAMP-response element binding protein

doi: 10.1080/15384047.2022.2094670

Figure Lengend Snippet: FABP5 inhibits the expression of miR-889-5p by regulating CREB protein phosphorylation. a, the siRNA of NF-κB, c-Myc and CREB were transiently transferred into HepG2 and SK-Hep-1 cells. The expression of miR-889-5p was detected by RT-qPCR. b, the designed mutative model of miR-889-5p promoter. c, a luciferase reporter promoter system was employed to confirm CREB bind to miR-889-5p promoter. d, the expression of total and phosphorylated CREB protein were detected by western blotting in the indicated groups. e, CREB inhibitor KG-501 reverse the effect of FABP5 overexpression on miR-889-5p expression in HCC cells. **p < .01 and ***p < .001 as compared with the vehicle control. e, the scheme of the mechanism by which FABP5 affects HCC tumorigenesis. FABP5 improves CREB protein phosphorylation to upregulate the miR-889-5p expression by CREB binding to the miR-889-5p promoter region, whereby leading to downregulation of KLF9 by miR-889-5p binding to the 3ʹ-UTR of the KLF9 mRNA, potentiating the PI3K/AKT signaling pathway and promoting the proliferation, migration, and invasion of HCC cells.

Article Snippet: The sections were incubated at 4°C overnight with rabbit anti-human FABP5 primary antibody (Proteintech, cat. no. 12348-1-AP) and then with goat anti-rabbit secondary antibody (Proteintech, cat. no. PR30009) for 50 min at room temperature.

Techniques: Expressing, Quantitative RT-PCR, Luciferase, Western Blot, Over Expression, Binding Assay, Migration