Journal: Oncogene

Article Title: Androgen regulation of soluble guanylyl cyclasealpha1 mediates prostate cancer cell proliferation.

doi: 10.1038/sj.onc.1209956

Figure Lengend Snippet: Figure 6 sGCa1 is overexpressed in advanced prostate cancer tissues. (a) Total mRNA was isolated from prostate tissues (acquired from CHTN), which are normal (N1), BPH (B1–B3), or MPC (C1, C2) and subjected to semi-quantitative RT-PCR to measure the expression of sGCa1, sGCb1, PSA, EZH2, E-cadherin, and AR. (b) The human prostate cancer tissue array slide (from Imgenex) was prepared and subjected to immunohistochemistry analysis to detect the expression of sGCa1 protein. Staining results from one or more representative tissues, of different stages, are shown. DAPI staining shows cell nuclei in the lower panels. (c) The sGCa1 expression levels of 45 tissue samples (five normal; eight stages 1 and 2; 32 stages 3 and 4) were quantified according to manufacturer’s protocol (Imgenex). Note that sGCa1 expression levels are represented relative to the average expression level of normal tissues, which was set to 1. Data represent mean expression values plus/minus s.d.

Article Snippet: Prostate tissue array slide (Imgenex) was processed according to the manufacturer’s protocol.

Techniques: Isolation, Quantitative RT-PCR, Expressing, Immunohistochemistry, Staining

Journal: International journal of cancer

Article Title: Increased expression of prostate-specific G-protein-coupled receptor in human prostate intraepithelial neoplasia and prostate cancers.

doi: 10.1002/ijc.20635

Figure Lengend Snippet: FIGURE 1 – Specific expression of PSGR in human prostate tissues using in situ hybridization. (A) Overexpression of PSGR in human prostate malignant tissues with different clinical stages and Gleason scores. (A-a) Gleason score 7, clinical stage II, T2N0M0. (A-b) Gleason score 4, clinical stage III, T3N0M0. (A-c) Gleason score 9, clinical stage IV, T4NXM1. (A-d) Gleason score 9, clinical stage IV, T3N1M0. (B) PSGR expression in prostate PIN and normal tissue. Strong PSGR expression is noted in varied stages of PIN (arrow) samples, whereas very low amount of PSGR transcripts are evident in adjacent normal prostate tissues (N, arrow). (B-a) Expression of PSGR in tufting pattern of PIN and normal prostate tissue. (B-b) PSGR expression in the micropapillary pattern of PIN lesion. (B-c and B-d) In situ hybridization of PSGR expression using PSGR sense probe as a control. (C) Expression of PSGR in nonprostate malignant tissues. No PSGR expression was detected in other malignant tissues. (C-a) Breast cancer tissue. (C-b) Lung cancer. (C-c) Stomach cancer. (C-d) Uterine cancer tissue.

Article Snippet: In situ hybridization of PSGR in prostate tissues Tissue slides containing 56 prostate tumors and 11 nonprostate tumors of varied clinical stages, 6 normal prostate tissues and 11 normal nonprostate tissues embedded in paraffin were bought from IMGENEX.

Techniques: Expressing, In Situ Hybridization, Over Expression, Control

Journal: International journal of cancer

Article Title: Increased expression of prostate-specific G-protein-coupled receptor in human prostate intraepithelial neoplasia and prostate cancers.

doi: 10.1002/ijc.20635

Figure Lengend Snippet: FIGURE 2 – The expression of PSGR mRNA level (copies/103 -ac- tin) in normal prostate tissue (normal, 19), BPH (8) and PCa (57) determined by real-time RT-PCR method. The expression level of PSGR mRNA detected in the prostate tumor tissue is significantly higher than that from the normal prostate tissue and BPH tissue (p 0.001, Mann-Whitney U-test). No significant difference was detected for the expression of PSGR between normal prostate and BPH (p 0.05, Mann-Whitney U-test).

Article Snippet: In situ hybridization of PSGR in prostate tissues Tissue slides containing 56 prostate tumors and 11 nonprostate tumors of varied clinical stages, 6 normal prostate tissues and 11 normal nonprostate tissues embedded in paraffin were bought from IMGENEX.

Techniques: Expressing, Quantitative RT-PCR, MANN-WHITNEY

Journal: International journal of cancer

Article Title: Increased expression of prostate-specific G-protein-coupled receptor in human prostate intraepithelial neoplasia and prostate cancers.

doi: 10.1002/ijc.20635

Figure Lengend Snippet: FIGURE 3 – ROC for PSGR real-time PCR assay. The assay has high-level sensitivity and specificity for discriminating benign prostate tissue from malignant prostate tissue in laboratory (AUC 0.902 0.032).

Article Snippet: In situ hybridization of PSGR in prostate tissues Tissue slides containing 56 prostate tumors and 11 nonprostate tumors of varied clinical stages, 6 normal prostate tissues and 11 normal nonprostate tissues embedded in paraffin were bought from IMGENEX.

Techniques: Real-time Polymerase Chain Reaction

Journal: Cancer science

Article Title: Coordinate suppression of Sdpr and Fhl1 expression in tumors of the breast, kidney, and prostate.

doi: 10.1111/j.1349-7006.2008.00816.x

Figure Lengend Snippet: Fig. 1. Fhl1 and Sdpr expression in normal epi- thelium and tumors from human breast, kidney, and prostate. Immunohistochemical staining was performed on normal and tumor human tissue. Fhl1 and Sdpr staining was evident in normal epithelial cells of the breast, kidney, and prostate. In contrast, both Fhl1 and Sdpr expression were suppressed in infiltrating mammary duct carcinoma, renal cell carcinoma, and prostate adenocarcinoma. (bar = 60 microns).

Article Snippet: Slides containing 15 infiltrating duct carcinoma specimens with normal adjacent tissue from breast cancer patients (IMH-371, Imgenex), nine prostate adenocarcinoma specimens with adjacent normal tissue and an additional unmatched 41 prostate adenocarcinoma specimens (IMH-303, Imgenex), and nine renal cell carcinoma specimens with adjacent normal tissue and an additional unmatched 31 cell carcinoma specimens (IMH-313, Imgenex) were examined by immunohistochemistry as previously described. (19) Briefly, after removal of paraffin with xylene and rehydration through a graded series of decreasing concentrations of ethanol, protein antigenicity was enhanced by microwaving sections in Target Buffer (Dako, Carpenturia, CA, USA) for 2 min.

Techniques: Expressing, Immunohistochemical staining, Staining