Journal: Journal of Clinical Medicine

Article Title: Skeletal Muscle Subpopulation Rearrangements upon Rhabdomyosarcoma Development through Single-Cell Mass Cytometry

doi: 10.3390/jcm10040823

Figure Lengend Snippet: Generation of eRMS tumors in LSL-Kras G12D/+ ;Tp53 Fl/Fl mice. ( A ) Experimental workflow. LSL-Kras G12D/+ ;Tp53 Fl/Fl conditional mice were injected intramuscularly with Ad-Cre in order to induce genetic rearrangements, the constitutive activation of Kras and the inactivation of Tp53, which lead to eRMS tumor masses in a spatially and temporally controlled manner. ( B ) LSL-Kras G12D/+ ;Tp53 Fl/Fl conditional mice were injected with Ad-Cre and Ctrl vector. Tumor growth was assessed at three, five and seven weeks after Ad-Cre infection through FMT performed by intravitreal administration of the fluorescent probe (ProSense 680). This probe was activated by tumor cathepsin proteases to yield a fluorescence signal (black: low cathepsin activity; white: high cathepsin activity). ( C ) Representative H&E staining on histological sections from Ctrl and Ad-Cre infected muscles at three, five and seven weeks after Ad infection. ( D , E ) Immunofluorescence staining on sections of Ad-Cre infected muscles at three, five and seven weeks after infection. Sections were stained with antibodies raised against Caveolin-3 ( D ) and α-SMA ( E ). Scale bars: 100 µm.

Article Snippet: The emerging tumors were imaged by injecting a fluorescent probe (ProSense 680) that was specifically activated by cathepsin proteases, a tumor-specific enzymatic activity [ ].

Techniques: Injection, Activation Assay, Plasmid Preparation, Infection, Fluorescence, Activity Assay, Staining, Muscles, Immunofluorescence

Journal: Molecular Imaging

Article Title: Ex Vivo and In Vivo Noninvasive Imaging of Epidermal Growth Factor Receptor Inhibition on Colon Tumorigenesis Using Activatable Near-Infrared Fluorescent Probes

doi: 10.1177/1536012117729044

Figure Lengend Snippet: Detection of decreased colonic tumor load in epidermal growth factor receptor (EGFR) inhibitor diet–fed azoxymethane (AOM)-treated KK-HIJ animals by 2-D fluorescence imaging and ProSense 680 activatable probe. A, The 2-D fluorescence reflectance imaging (FRI) images demonstrate reduced tumor load in EGFR inhibitor diet–fed animals. B, The 2-D quantification analysis suggests significant lowered FRI signals in the colon of EGFR inhibitor diet–fed mice versus chow diet–fed animals. C, Graph of distal colon 2-D FRI signal intensity (counts/energy) and tumor load in each animal indicates EGFR inhibitor diet–fed animals have smaller and less colonic tumors. Each dot represents individual animal (red dots represent animals from EGFR inhibitor diet–fed group, green dots represent animals from chow diet–fed group). D and E, Graph of 2-D FRI signal intensity (counts/energy) and lesion size for individual lesion from chow diet–fed animals (D, green plot graph) and EGFR inhibitor diet–fed animals (E, red plot graph).

Article Snippet: Ex vivo imaging was performed on whole colon of KK-HIJ animals 24 hours after ProSense 680 probe injection.

Techniques: Fluorescence, Imaging

Journal: Molecular Imaging

Article Title: Ex Vivo and In Vivo Noninvasive Imaging of Epidermal Growth Factor Receptor Inhibition on Colon Tumorigenesis Using Activatable Near-Infrared Fluorescent Probes

doi: 10.1177/1536012117729044

Figure Lengend Snippet: The 2-D ex vivo imaging with ProSense 680 activatable probe can differentiate large nuclear β-catenin-positive tumors versus small lesions, but not small tumors and lymphoid follicle from normal epithelium. A, Representative 2-D fluorescence reflectance imaging (FRI) images of lesions in each category. B, β-Catenin immunostaining photomicrographs to illustrate lesions in each category (1-4) based on the grading scheme in . C, Scatter plot of each categorized lesion based on 2-D fluorescence signal intensity (counts/energy). * P < .05 versus categories 1, 2 and 3.

Article Snippet: Ex vivo imaging was performed on whole colon of KK-HIJ animals 24 hours after ProSense 680 probe injection.

Techniques: Ex Vivo, Imaging, Fluorescence, Immunostaining

Journal: Molecular Imaging

Article Title: Ex Vivo and In Vivo Noninvasive Imaging of Epidermal Growth Factor Receptor Inhibition on Colon Tumorigenesis Using Activatable Near-Infrared Fluorescent Probes

doi: 10.1177/1536012117729044

Figure Lengend Snippet: Three-dimensional (3-D) in vivo fluorescence tomography imaging using ProSense 680 molecular probe is not effective to detect the reduction of colonic tumor load in epidermal growth factor receptor (EGFR)-inhibitor-diet-treated azoxymethane (AOM)-injected animals. A, Representative 3-D Fluorescence Molecular Tomography (FMT) images from chow diet– and EGFR inhibitor diet–fed animals. Region of interest (ROI) in purple is placed in abdominal area. B, Three-dimensional (3D) quantification by FMT in abdominal area (ROI volumes are the same across the animals) suggests no difference in fluorescence signal intensity between chow-diet and EGFR inhibitor diet–fed animals. C, Representative 3-D FMT images from chow-diet and EGFR inhibitor diet–fed animals. Region of interest in purple is placed in the colon area. D, Three-dimensional quantification by FMT in the colon area suggests no difference in fluorescence signal intensity between chow-diet and EGFR inhibitor diet–fed animals.

Article Snippet: Ex vivo imaging was performed on whole colon of KK-HIJ animals 24 hours after ProSense 680 probe injection.

Techniques: In Vivo, Fluorescence, Tomography, Imaging, Injection

Journal: Molecular Imaging

Article Title: Ex Vivo and In Vivo Noninvasive Imaging of Epidermal Growth Factor Receptor Inhibition on Colon Tumorigenesis Using Activatable Near-Infrared Fluorescent Probes

doi: 10.1177/1536012117729044

Figure Lengend Snippet: In vivo 3-dimensional (3D) fluorescence imaging using ProSense 680 activatable probe can detect abdominal tumor load in Apc Min/+ mice. A, Representative 3-D in vivo fluorescence images of Apc Min/+ mice and nontumor control mice injected with ProSense 680 probe. Region of interest (ROI) in purple is placed in abdominal area. B, Three-dimensional quantification results in the abdominal area indicate significant higher fluorescence signals in Apc Min/+ mice than nontumor control animals. Data are expressed as mean ± standard error (SE). n = 4 to 5/group. * P < .05 versus nontumor control mice. C and D, Linear regression analysis on ex vivo 2-D whole intestine fluorescence signal intensity and in vivo 3-D abdominal fluorescence signal intensity suggests no significant correlation in control mice (C) or Apc Min/+ mice (D).

Article Snippet: Ex vivo imaging was performed on whole colon of KK-HIJ animals 24 hours after ProSense 680 probe injection.

Techniques: In Vivo, Fluorescence, Imaging, Injection, Ex Vivo

Journal: Molecular Imaging

Article Title: Ex Vivo and In Vivo Noninvasive Imaging of Epidermal Growth Factor Receptor Inhibition on Colon Tumorigenesis Using Activatable Near-Infrared Fluorescent Probes

doi: 10.1177/1536012117729044

Figure Lengend Snippet: Dual-model microCT/FMT imaging on intestinal adenomas in Apc Min/+ mice. A, Tumor nodules were detected by contrast-enhanced microCT imaging (white arrows). B, Coregistration of microCT image with 3-dimensional (3D) fluorescence image activated by ProSense 680 probe. Three-dimensional image was taken at 24 hours after injection of ProSense 680 activatable probe and fused with microCT image for anatomical colocalization. C, Two-dimensional fluorescence reflectance imaging (FRI) images of small intestine. After coregistration of in vivo imaging, the small intestine was dissected and imaged ex vivo by FRI for validation.

Article Snippet: Ex vivo imaging was performed on whole colon of KK-HIJ animals 24 hours after ProSense 680 probe injection.

Techniques: Imaging, Fluorescence, Injection, In Vivo Imaging, Ex Vivo