Journal: Journal of Biological Chemistry

Article Title: Suberoylanilide Hydroxamic Acid Reactivates HIV from Latently Infected Cells

doi: 10.1074/jbc.m807898200

Figure Lengend Snippet: FIGURE 3. SAHA releases active P-TEFb. Jurkat T cells were untreated or treated with SAHA. At 30 min and 1, 2, or 6 h, cell lysates were subjected to glycerol gradient centrifugation. Fractions were collected and proteins pre- cipitated before Western blotting with anti-HEXIM1 (bottom panels) and anti- Cdk9 (top panels) antibodies. Fractions 1–3 contain the active SC, whereas fractions 5–7 contain the inactive LC.

Article Snippet: Glycerol Gradient Sedimentation Analysis—Glycerol gradients (10–30%) were established by pipetting 2ml of each of the glycerol fractions (10, 15, 20, 25, and 30% v/v) in buffer A (20 mM HEPES, pH 7.9, 0.3 M KCl, 0.2 mM EDTA, 0.1% Nonidet P-40) into centrifugation tubes (catalog number 331372, Beckman Instruments, Palo Alto, CA).

Techniques: Gradient Centrifugation, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: The human innate immune protein calprotectin induces iron starvation responses in Pseudomonas aeruginosa

doi: 10.1074/jbc.RA118.006819

Figure Lengend Snippet: P. aeruginosa metabolites affect iron depletion by CP. Tris/TSB was supplemented with 10 μm CP-Ser in the absence or presence of 5 μm PYO (A) or 2 μm pyoverdine (Pvd) (B) and incubated at 37 °C. At 4 and 8 h, CP-Ser was filtered out, and the metal content in the flow-through was analyzed by ICP-MS. The t = 0 h time point corresponds to filtered media before any CP, PYO, or pyoverdine supplementation and is thus the same for each condition (n = 3; **, p < 0.01).

Article Snippet: Samples were prepared in 15-ml Falcon tubes, and 2-ml samples were transferred to ICP-MS polypropylene vials (PerkinElmer Life Sciences, B3001566) and analyzed.

Techniques: Incubation