Journal: Experimental diabetes research

Article Title: Pentoxifylline attenuates methionine- and choline-deficient-diet-induced steatohepatitis by suppressing TNF-α expression and endoplasmic reticulum stress.

doi: 10.1155/2012/762565

Figure Lengend Snippet: Figure 5: Effect of pentoxifylline on TNF-α-induced ER stress in Hep3B cells and hepatocytes. Rats fed MCD diet (group III) showed activated levels of GRP78, phosphor-eIF2α, ATF4, ATF6, IRE1, p-JNK, and CHOP with significance in ATF4, ATF6, and IRE1. Compared to group III, administration of PTX in MCD diet rats (group IV) attenuated the expression of GRP78, phosphor-eIF2α, ATF4, ATF6, p-JNK, and CHOP (a). The experiments were performed five times under identical conditions. Results are shown as mean ± SEM. ∗∗∗P < 0.001 versus untreated control group. †P < 0.05, ††P < 0.001: MCD plus saline versus MCD plus PTX group. Hep3B cells exposed to 100 ng/mL TNF-α for 24 h activated GRP78, p-eIF2α, ATF4, ATF6, IRE1, p-JNK, and CHOP. Pretreatment of 1 mM PTX for 18 and 24 h reduced TNF-α-induced ER stress in Hep3B cells (b). The experiments were performed four times under identical conditions.

Article Snippet: After blocking with 5% skim milk in 1XPBS, the membranes were incubated with each specific primary antibody, including GRP78 (Santa Cruz Biotechnology, Santa Cruz, nloaded from https://onlinelibrary.w iley.com /doi/10.1155/2012/762565 by N at Prov Indonesia, W iley O nline L ibrary on [17/09/2024].

Techniques: Expressing, Control, Saline