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DNASTAR cycloartenol synthases
Phylogenetic relationships of selected functionally characterized oxidosqualene cyclases from plants. Amino acid sequences were aligned in ClustalW, and the phylogenetic tree was constructed using the neighbor-joining method with 500 bootstrap iterations in MEGAX. HgOSC1 showed a close relationship with lupeol <t>synthases,</t> whereas HgOSC2 formed a clade with other previously characterized <t>cycloartenol</t> synthases.
Cycloartenol Synthases, supplied by DNASTAR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DNASTAR seqman dnastar software
Phylogenetic relationships of selected functionally characterized oxidosqualene cyclases from plants. Amino acid sequences were aligned in ClustalW, and the phylogenetic tree was constructed using the neighbor-joining method with 500 bootstrap iterations in MEGAX. HgOSC1 showed a close relationship with lupeol <t>synthases,</t> whereas HgOSC2 formed a clade with other previously characterized <t>cycloartenol</t> synthases.
Seqman Dnastar Software, supplied by DNASTAR, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DNASTAR seqbuilder pro program
Phylogenetic relationships of selected functionally characterized oxidosqualene cyclases from plants. Amino acid sequences were aligned in ClustalW, and the phylogenetic tree was constructed using the neighbor-joining method with 500 bootstrap iterations in MEGAX. HgOSC1 showed a close relationship with lupeol <t>synthases,</t> whereas HgOSC2 formed a clade with other previously characterized <t>cycloartenol</t> synthases.
Seqbuilder Pro Program, supplied by DNASTAR, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vazyme Biotech Co taq universal sybr mix
Phylogenetic relationships of selected functionally characterized oxidosqualene cyclases from plants. Amino acid sequences were aligned in ClustalW, and the phylogenetic tree was constructed using the neighbor-joining method with 500 bootstrap iterations in MEGAX. HgOSC1 showed a close relationship with lupeol <t>synthases,</t> whereas HgOSC2 formed a clade with other previously characterized <t>cycloartenol</t> synthases.
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R&D Systems quantinine mmp900
Phylogenetic relationships of selected functionally characterized oxidosqualene cyclases from plants. Amino acid sequences were aligned in ClustalW, and the phylogenetic tree was constructed using the neighbor-joining method with 500 bootstrap iterations in MEGAX. HgOSC1 showed a close relationship with lupeol <t>synthases,</t> whereas HgOSC2 formed a clade with other previously characterized <t>cycloartenol</t> synthases.
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R&D Systems human matrix metalloproteinase 1 duoset elisa kit
Phylogenetic relationships of selected functionally characterized oxidosqualene cyclases from plants. Amino acid sequences were aligned in ClustalW, and the phylogenetic tree was constructed using the neighbor-joining method with 500 bootstrap iterations in MEGAX. HgOSC1 showed a close relationship with lupeol <t>synthases,</t> whereas HgOSC2 formed a clade with other previously characterized <t>cycloartenol</t> synthases.
Human Matrix Metalloproteinase 1 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems mouse mmp 9
Phylogenetic relationships of selected functionally characterized oxidosqualene cyclases from plants. Amino acid sequences were aligned in ClustalW, and the phylogenetic tree was constructed using the neighbor-joining method with 500 bootstrap iterations in MEGAX. HgOSC1 showed a close relationship with lupeol <t>synthases,</t> whereas HgOSC2 formed a clade with other previously characterized <t>cycloartenol</t> synthases.
Mouse Mmp 9, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Phylogenetic relationships of selected functionally characterized oxidosqualene cyclases from plants. Amino acid sequences were aligned in ClustalW, and the phylogenetic tree was constructed using the neighbor-joining method with 500 bootstrap iterations in MEGAX. HgOSC1 showed a close relationship with lupeol synthases, whereas HgOSC2 formed a clade with other previously characterized cycloartenol synthases.

Journal: Plants

Article Title: Identification and Functional Characterization of Oxidosqualene Cyclases from Medicinal Plant Hoodia gordonii

doi: 10.3390/plants13020231

Figure Lengend Snippet: Phylogenetic relationships of selected functionally characterized oxidosqualene cyclases from plants. Amino acid sequences were aligned in ClustalW, and the phylogenetic tree was constructed using the neighbor-joining method with 500 bootstrap iterations in MEGAX. HgOSC1 showed a close relationship with lupeol synthases, whereas HgOSC2 formed a clade with other previously characterized cycloartenol synthases.

Article Snippet: The two OSC proteins were aligned with previously published plant lupeol and cycloartenol synthases and analyzed in MegAlign Pro (DNA Star v15).

Techniques: Construct

Total ion chromatogram (TIC) of GC/MS analysis of yeast cell extracts. TICs of extracts from the yeast strain transformed with pYES2 - HgOSC2 (A) and vector pYES2 as a control (B). Compounds identified were: 1, squalene; 2, ergosterol; 3, cycloartenol; and 4, cycloartenol acetate. The compound identification was based on the spectra search with NIST and Wiley libraries, and further confirmed with reference standards.

Journal: Plants

Article Title: Identification and Functional Characterization of Oxidosqualene Cyclases from Medicinal Plant Hoodia gordonii

doi: 10.3390/plants13020231

Figure Lengend Snippet: Total ion chromatogram (TIC) of GC/MS analysis of yeast cell extracts. TICs of extracts from the yeast strain transformed with pYES2 - HgOSC2 (A) and vector pYES2 as a control (B). Compounds identified were: 1, squalene; 2, ergosterol; 3, cycloartenol; and 4, cycloartenol acetate. The compound identification was based on the spectra search with NIST and Wiley libraries, and further confirmed with reference standards.

Article Snippet: The two OSC proteins were aligned with previously published plant lupeol and cycloartenol synthases and analyzed in MegAlign Pro (DNA Star v15).

Techniques: Gas Chromatography-Mass Spectrometry, Transformation Assay, Plasmid Preparation