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Image Search Results
Journal: Plants
Article Title: Identification and Functional Characterization of Oxidosqualene Cyclases from Medicinal Plant Hoodia gordonii
doi: 10.3390/plants13020231
Figure Lengend Snippet: Phylogenetic relationships of selected functionally characterized oxidosqualene cyclases from plants. Amino acid sequences were aligned in ClustalW, and the phylogenetic tree was constructed using the neighbor-joining method with 500 bootstrap iterations in MEGAX. HgOSC1 showed a close relationship with lupeol synthases, whereas HgOSC2 formed a clade with other previously characterized cycloartenol synthases.
Article Snippet: The two OSC proteins were aligned with previously published plant lupeol and
Techniques: Construct
Journal: Plants
Article Title: Identification and Functional Characterization of Oxidosqualene Cyclases from Medicinal Plant Hoodia gordonii
doi: 10.3390/plants13020231
Figure Lengend Snippet: Total ion chromatogram (TIC) of GC/MS analysis of yeast cell extracts. TICs of extracts from the yeast strain transformed with pYES2 - HgOSC2 (A) and vector pYES2 as a control (B). Compounds identified were: 1, squalene; 2, ergosterol; 3, cycloartenol; and 4, cycloartenol acetate. The compound identification was based on the spectra search with NIST and Wiley libraries, and further confirmed with reference standards.
Article Snippet: The two OSC proteins were aligned with previously published plant lupeol and
Techniques: Gas Chromatography-Mass Spectrometry, Transformation Assay, Plasmid Preparation