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Thermo Fisher
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Genecopoeia
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Proteintech
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Atlas Antibodies
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ProSci Incorporated
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International Federation of Clinical Chemistry and Laboratory Medicine
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USCN Life
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Ribobio co
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MyBiosource Biotechnology
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GenScript corporation
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SHAL Technologies Inc
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Hamad Medical Corporation
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Image Search Results
Journal: Communications Biology
Article Title: Lysine malonylation regulates human sperm motility
doi: 10.1038/s42003-026-09683-y
Figure Lengend Snippet: A The Kmal in total proteins of (TP) and protein fractions of the mitochondrion (Mit), head, and cytoplasm (Cyt) isolated from human sperm were examined by Western blot. The specificity of cellular components was validated by Western blot using the corresponding markers: PRM2 for sperm head, COX6B1 for mitochondrion, and ACTIN for cytoplasm. The proteins for Western blot were shown in the coomassie brilliant blue (CBB) staining image. B , C The localization of malonylated proteins within human sperm was investigated by immunofluorescence assay via laser scanning confocal microscopy (LSCM, ( B )) and super-resolution structured illumination microscopy (SIM, ( C )). All the experiments were conducted with samples from 8 normozoospermic men. The scale bar represents 5 μm.
Article Snippet: Anti-ACTIN antibody (66009-1-Ig), anti-SIRT5 antibody (67257-1-Ig), anti-acetyl-CoA carboxylase 1 (ACC1) antibody (21923-1-AP), anti-fatty acid synthase (FASN) antibody (10624-2-AP),
Techniques: Isolation, Western Blot, Staining, Immunofluorescence, Confocal Microscopy, Microscopy
Journal: Veterinary World
Article Title: The potential of sperm bovine protamine as a protein marker of semen production and quality at the National Artificial Insemination Center of Indonesia
doi: 10.14202/vetworld.2021.2473-2481
Figure Lengend Snippet: Bovine Protamine 1 (PRM1) exhibited the highest concentration (p<0.00) in the sperm of Limousin, Friesian Holsten, Peranakan Ongole, and Aceh bulls, followed by PRM2 (p<0.00) and PRM3 (p<0.00).
Article Snippet: A total of 100 mL of thawed semen was centrifuged at 3000 rpm for 15 min, washed with a solution of PBS twice, and used to measure the concentration of PRM2 using a
Techniques: Concentration Assay
Journal: Veterinary World
Article Title: The potential of sperm bovine protamine as a protein marker of semen production and quality at the National Artificial Insemination Center of Indonesia
doi: 10.14202/vetworld.2021.2473-2481
Figure Lengend Snippet: Relationship between the Protamine (PRM1), PRM2, and PRM3 concentrations and frozen semen production capacity in Limousin, Friesian Holstein (FH), Peranakan Ongole (PO), and Aceh bulls (a-c). The PRM1 concentrations were higher (p<0.05) in the high production groups in Limousin, FH, PO, and Aceh bulls (a). The concentrations of PRM2 and PRM3 varied among the production groups in all bull breeds (b and c). Results of DNA fragmentation staining in sperm using acridine orange (d); sperm with DNA fragmentation exhibited yellow-orange fluorescence (a), and normal sperm showed green fluorescence (b).
Article Snippet: A total of 100 mL of thawed semen was centrifuged at 3000 rpm for 15 min, washed with a solution of PBS twice, and used to measure the concentration of PRM2 using a
Techniques: Staining, Fluorescence
Journal: Veterinary World
Article Title: The potential of sperm bovine protamine as a protein marker of semen production and quality at the National Artificial Insemination Center of Indonesia
doi: 10.14202/vetworld.2021.2473-2481
Figure Lengend Snippet: Correlation between PRM1, PRM2, and PRM3 and semen quality parameters in Limousine, FH, PO, and Aceh bulls.
Article Snippet: A total of 100 mL of thawed semen was centrifuged at 3000 rpm for 15 min, washed with a solution of PBS twice, and used to measure the concentration of PRM2 using a
Techniques: