primary human monocytes Search Results


peripheral blood cells  (ATCC)
99
ATCC peripheral blood cells
Peripheral Blood Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+monocytes/Primary+Peripheral+Blood+CD14%2B+Monocytes%2C+Normal%2C+Human/pmc05305699-51-1-4
Average 99 stars, based on 1 article reviews
peripheral blood cells - by Bioz Stars, 2026-11
99/100 stars
  Buy from Supplier
primary human cd14 monocytes  (ATCC)
93
ATCC primary human cd14 monocytes
(A) FLAG immunoprecipitation was performed from lysates of HEK293T cells expressing FLAG-RNF213 or FLAG-eGFP in combination with HA-ISG15(AA) and the ISGylation machinery (E1, E2, E3). A smear of ISGylated co-immunoprecipitated proteins was detected with FLAG-RNF213, but not with FLAG-eGFP or when non-conjugatable HA-ISG15AA was used. (B) . THP-1 or primary human monocytes <t>(CD14+)</t> cells were cultured in the presence of 10 mM BSA-conjugated oleic acid and either treated with 10 ng/mL interferon-β for 8 h or left untreated. Lipid droplets (LDs)-enriched fractions were isolated by ultracentrifugation floatation assay on a sucrose step-gradient. Immunoblots against RNF213 and ISG15 revealed an interferon-induced upregulation of both proteins on LDs and a smear of ISGylated proteins associated with LDs. Immunoblots against PLIN1, PLIN2, ATGL and GAPDH confirmed LD isolation and equal protein loading in the lysate and LD-enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded). (C) Similarly, LDs were isolated from THP1 cells after knockdown of RNF213 by siRNA treatment for 48h or using a non-targeting scrambled siRNA (siScramble) as control. Immunoblotting against ISG15 revealed a smear of ISGylated proteins associated with LDs only when RNF213 was present. Immunoblotting against RNF213 confirmed knockdown of RNF213, while PLIN1, PLIN2, ATGL and GAPDH validated LD isolation and equal protein loading in the lysate and the LD- enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded).
Primary Human Cd14 Monocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+monocytes/Primary+Peripheral+Blood+CD14%2B+Monocytes%2C+Normal%2C+Human/bio_rxiv__2021__06__03__446796-311-22-26
Average 93 stars, based on 1 article reviews
primary human cd14 monocytes - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
human monocyte-derived primary dcs 70041  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc human monocyte-derived primary dcs 70041
(A) FLAG immunoprecipitation was performed from lysates of HEK293T cells expressing FLAG-RNF213 or FLAG-eGFP in combination with HA-ISG15(AA) and the ISGylation machinery (E1, E2, E3). A smear of ISGylated co-immunoprecipitated proteins was detected with FLAG-RNF213, but not with FLAG-eGFP or when non-conjugatable HA-ISG15AA was used. (B) . THP-1 or primary human monocytes <t>(CD14+)</t> cells were cultured in the presence of 10 mM BSA-conjugated oleic acid and either treated with 10 ng/mL interferon-β for 8 h or left untreated. Lipid droplets (LDs)-enriched fractions were isolated by ultracentrifugation floatation assay on a sucrose step-gradient. Immunoblots against RNF213 and ISG15 revealed an interferon-induced upregulation of both proteins on LDs and a smear of ISGylated proteins associated with LDs. Immunoblots against PLIN1, PLIN2, ATGL and GAPDH confirmed LD isolation and equal protein loading in the lysate and LD-enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded). (C) Similarly, LDs were isolated from THP1 cells after knockdown of RNF213 by siRNA treatment for 48h or using a non-targeting scrambled siRNA (siScramble) as control. Immunoblotting against ISG15 revealed a smear of ISGylated proteins associated with LDs only when RNF213 was present. Immunoblotting against RNF213 confirmed knockdown of RNF213, while PLIN1, PLIN2, ATGL and GAPDH validated LD isolation and equal protein loading in the lysate and the LD- enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded).
Human Monocyte Derived Primary Dcs 70041, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+monocytes/human+monocyte+derived+primary+dcs+70041/pmc07264239-360-3-4
Average 90 stars, based on 1 article reviews
human monocyte-derived primary dcs 70041 - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
primary human monocytes  (Institute for Clinical Pharmacodynamics)
90
Institute for Clinical Pharmacodynamics primary human monocytes
(A) FLAG immunoprecipitation was performed from lysates of HEK293T cells expressing FLAG-RNF213 or FLAG-eGFP in combination with HA-ISG15(AA) and the ISGylation machinery (E1, E2, E3). A smear of ISGylated co-immunoprecipitated proteins was detected with FLAG-RNF213, but not with FLAG-eGFP or when non-conjugatable HA-ISG15AA was used. (B) . THP-1 or primary human monocytes <t>(CD14+)</t> cells were cultured in the presence of 10 mM BSA-conjugated oleic acid and either treated with 10 ng/mL interferon-β for 8 h or left untreated. Lipid droplets (LDs)-enriched fractions were isolated by ultracentrifugation floatation assay on a sucrose step-gradient. Immunoblots against RNF213 and ISG15 revealed an interferon-induced upregulation of both proteins on LDs and a smear of ISGylated proteins associated with LDs. Immunoblots against PLIN1, PLIN2, ATGL and GAPDH confirmed LD isolation and equal protein loading in the lysate and LD-enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded). (C) Similarly, LDs were isolated from THP1 cells after knockdown of RNF213 by siRNA treatment for 48h or using a non-targeting scrambled siRNA (siScramble) as control. Immunoblotting against ISG15 revealed a smear of ISGylated proteins associated with LDs only when RNF213 was present. Immunoblotting against RNF213 confirmed knockdown of RNF213, while PLIN1, PLIN2, ATGL and GAPDH validated LD isolation and equal protein loading in the lysate and the LD- enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded).
Primary Human Monocytes, supplied by Institute for Clinical Pharmacodynamics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+monocytes/primary+human+monocytes/pmc06730955-151-0-12
Average 90 stars, based on 1 article reviews
primary human monocytes - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
mrna transfection-induced activation of primary human monocytes and macrophages  (Helmholtz Zentrum fur Infektionsforschung GmbH)
90
Helmholtz Zentrum fur Infektionsforschung GmbH mrna transfection-induced activation of primary human monocytes and macrophages
(A) FLAG immunoprecipitation was performed from lysates of HEK293T cells expressing FLAG-RNF213 or FLAG-eGFP in combination with HA-ISG15(AA) and the ISGylation machinery (E1, E2, E3). A smear of ISGylated co-immunoprecipitated proteins was detected with FLAG-RNF213, but not with FLAG-eGFP or when non-conjugatable HA-ISG15AA was used. (B) . THP-1 or primary human monocytes <t>(CD14+)</t> cells were cultured in the presence of 10 mM BSA-conjugated oleic acid and either treated with 10 ng/mL interferon-β for 8 h or left untreated. Lipid droplets (LDs)-enriched fractions were isolated by ultracentrifugation floatation assay on a sucrose step-gradient. Immunoblots against RNF213 and ISG15 revealed an interferon-induced upregulation of both proteins on LDs and a smear of ISGylated proteins associated with LDs. Immunoblots against PLIN1, PLIN2, ATGL and GAPDH confirmed LD isolation and equal protein loading in the lysate and LD-enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded). (C) Similarly, LDs were isolated from THP1 cells after knockdown of RNF213 by siRNA treatment for 48h or using a non-targeting scrambled siRNA (siScramble) as control. Immunoblotting against ISG15 revealed a smear of ISGylated proteins associated with LDs only when RNF213 was present. Immunoblotting against RNF213 confirmed knockdown of RNF213, while PLIN1, PLIN2, ATGL and GAPDH validated LD isolation and equal protein loading in the lysate and the LD- enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded).
Mrna Transfection Induced Activation Of Primary Human Monocytes And Macrophages, supplied by Helmholtz Zentrum fur Infektionsforschung GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+monocytes/mrna+transfection+induced+activation+of+primary+human+monocytes+and+macrophages/pmc07060354__41598_2020_60506_MOESM1_ESM-1-4-35
Average 90 stars, based on 1 article reviews
mrna transfection-induced activation of primary human monocytes and macrophages - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
primary human monocytes  (StemCells Inc)
90
StemCells Inc primary human monocytes
(A) FLAG immunoprecipitation was performed from lysates of HEK293T cells expressing FLAG-RNF213 or FLAG-eGFP in combination with HA-ISG15(AA) and the ISGylation machinery (E1, E2, E3). A smear of ISGylated co-immunoprecipitated proteins was detected with FLAG-RNF213, but not with FLAG-eGFP or when non-conjugatable HA-ISG15AA was used. (B) . THP-1 or primary human monocytes <t>(CD14+)</t> cells were cultured in the presence of 10 mM BSA-conjugated oleic acid and either treated with 10 ng/mL interferon-β for 8 h or left untreated. Lipid droplets (LDs)-enriched fractions were isolated by ultracentrifugation floatation assay on a sucrose step-gradient. Immunoblots against RNF213 and ISG15 revealed an interferon-induced upregulation of both proteins on LDs and a smear of ISGylated proteins associated with LDs. Immunoblots against PLIN1, PLIN2, ATGL and GAPDH confirmed LD isolation and equal protein loading in the lysate and LD-enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded). (C) Similarly, LDs were isolated from THP1 cells after knockdown of RNF213 by siRNA treatment for 48h or using a non-targeting scrambled siRNA (siScramble) as control. Immunoblotting against ISG15 revealed a smear of ISGylated proteins associated with LDs only when RNF213 was present. Immunoblotting against RNF213 confirmed knockdown of RNF213, while PLIN1, PLIN2, ATGL and GAPDH validated LD isolation and equal protein loading in the lysate and the LD- enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded).
Primary Human Monocytes, supplied by StemCells Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+monocytes/primary+human+monocytes/pmc11091880-188-2-10
Average 90 stars, based on 1 article reviews
primary human monocytes - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
primary human monocytes # 70,034  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc primary human monocytes # 70,034
(A) FLAG immunoprecipitation was performed from lysates of HEK293T cells expressing FLAG-RNF213 or FLAG-eGFP in combination with HA-ISG15(AA) and the ISGylation machinery (E1, E2, E3). A smear of ISGylated co-immunoprecipitated proteins was detected with FLAG-RNF213, but not with FLAG-eGFP or when non-conjugatable HA-ISG15AA was used. (B) . THP-1 or primary human monocytes <t>(CD14+)</t> cells were cultured in the presence of 10 mM BSA-conjugated oleic acid and either treated with 10 ng/mL interferon-β for 8 h or left untreated. Lipid droplets (LDs)-enriched fractions were isolated by ultracentrifugation floatation assay on a sucrose step-gradient. Immunoblots against RNF213 and ISG15 revealed an interferon-induced upregulation of both proteins on LDs and a smear of ISGylated proteins associated with LDs. Immunoblots against PLIN1, PLIN2, ATGL and GAPDH confirmed LD isolation and equal protein loading in the lysate and LD-enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded). (C) Similarly, LDs were isolated from THP1 cells after knockdown of RNF213 by siRNA treatment for 48h or using a non-targeting scrambled siRNA (siScramble) as control. Immunoblotting against ISG15 revealed a smear of ISGylated proteins associated with LDs only when RNF213 was present. Immunoblotting against RNF213 confirmed knockdown of RNF213, while PLIN1, PLIN2, ATGL and GAPDH validated LD isolation and equal protein loading in the lysate and the LD- enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded).
Primary Human Monocytes # 70,034, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+monocytes/primary+human+monocytes+++70+034/pmc08854169-154-16-25
Average 90 stars, based on 1 article reviews
primary human monocytes # 70,034 - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
peripheral blood (pb) primary human monocytes  (StemCells Inc)
90
StemCells Inc peripheral blood (pb) primary human monocytes
(A) FLAG immunoprecipitation was performed from lysates of HEK293T cells expressing FLAG-RNF213 or FLAG-eGFP in combination with HA-ISG15(AA) and the ISGylation machinery (E1, E2, E3). A smear of ISGylated co-immunoprecipitated proteins was detected with FLAG-RNF213, but not with FLAG-eGFP or when non-conjugatable HA-ISG15AA was used. (B) . THP-1 or primary human monocytes <t>(CD14+)</t> cells were cultured in the presence of 10 mM BSA-conjugated oleic acid and either treated with 10 ng/mL interferon-β for 8 h or left untreated. Lipid droplets (LDs)-enriched fractions were isolated by ultracentrifugation floatation assay on a sucrose step-gradient. Immunoblots against RNF213 and ISG15 revealed an interferon-induced upregulation of both proteins on LDs and a smear of ISGylated proteins associated with LDs. Immunoblots against PLIN1, PLIN2, ATGL and GAPDH confirmed LD isolation and equal protein loading in the lysate and LD-enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded). (C) Similarly, LDs were isolated from THP1 cells after knockdown of RNF213 by siRNA treatment for 48h or using a non-targeting scrambled siRNA (siScramble) as control. Immunoblotting against ISG15 revealed a smear of ISGylated proteins associated with LDs only when RNF213 was present. Immunoblotting against RNF213 confirmed knockdown of RNF213, while PLIN1, PLIN2, ATGL and GAPDH validated LD isolation and equal protein loading in the lysate and the LD- enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded).
Peripheral Blood (Pb) Primary Human Monocytes, supplied by StemCells Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+monocytes/peripheral+blood++pb++primary+human+monocytes/pm38602352-206-2-10
Average 90 stars, based on 1 article reviews
peripheral blood (pb) primary human monocytes - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
primary human total rna derived from hscs, monocytes and dcs  (BioChain Institute)
90
BioChain Institute primary human total rna derived from hscs, monocytes and dcs
(A) FLAG immunoprecipitation was performed from lysates of HEK293T cells expressing FLAG-RNF213 or FLAG-eGFP in combination with HA-ISG15(AA) and the ISGylation machinery (E1, E2, E3). A smear of ISGylated co-immunoprecipitated proteins was detected with FLAG-RNF213, but not with FLAG-eGFP or when non-conjugatable HA-ISG15AA was used. (B) . THP-1 or primary human monocytes <t>(CD14+)</t> cells were cultured in the presence of 10 mM BSA-conjugated oleic acid and either treated with 10 ng/mL interferon-β for 8 h or left untreated. Lipid droplets (LDs)-enriched fractions were isolated by ultracentrifugation floatation assay on a sucrose step-gradient. Immunoblots against RNF213 and ISG15 revealed an interferon-induced upregulation of both proteins on LDs and a smear of ISGylated proteins associated with LDs. Immunoblots against PLIN1, PLIN2, ATGL and GAPDH confirmed LD isolation and equal protein loading in the lysate and LD-enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded). (C) Similarly, LDs were isolated from THP1 cells after knockdown of RNF213 by siRNA treatment for 48h or using a non-targeting scrambled siRNA (siScramble) as control. Immunoblotting against ISG15 revealed a smear of ISGylated proteins associated with LDs only when RNF213 was present. Immunoblotting against RNF213 confirmed knockdown of RNF213, while PLIN1, PLIN2, ATGL and GAPDH validated LD isolation and equal protein loading in the lysate and the LD- enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded).
Primary Human Total Rna Derived From Hscs, Monocytes And Dcs, supplied by BioChain Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+monocytes/primary+human+total+rna+derived+from+hscs++monocytes+and+dcs/pmc10932245-213-9-13
Average 90 stars, based on 1 article reviews
primary human total rna derived from hscs, monocytes and dcs - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
primary human cd14 + monocytes  (Voden Medical Instruments)
90
Voden Medical Instruments primary human cd14 + monocytes
(A) FLAG immunoprecipitation was performed from lysates of HEK293T cells expressing FLAG-RNF213 or FLAG-eGFP in combination with HA-ISG15(AA) and the ISGylation machinery (E1, E2, E3). A smear of ISGylated co-immunoprecipitated proteins was detected with FLAG-RNF213, but not with FLAG-eGFP or when non-conjugatable HA-ISG15AA was used. (B) . THP-1 or primary human monocytes <t>(CD14+)</t> cells were cultured in the presence of 10 mM BSA-conjugated oleic acid and either treated with 10 ng/mL interferon-β for 8 h or left untreated. Lipid droplets (LDs)-enriched fractions were isolated by ultracentrifugation floatation assay on a sucrose step-gradient. Immunoblots against RNF213 and ISG15 revealed an interferon-induced upregulation of both proteins on LDs and a smear of ISGylated proteins associated with LDs. Immunoblots against PLIN1, PLIN2, ATGL and GAPDH confirmed LD isolation and equal protein loading in the lysate and LD-enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded). (C) Similarly, LDs were isolated from THP1 cells after knockdown of RNF213 by siRNA treatment for 48h or using a non-targeting scrambled siRNA (siScramble) as control. Immunoblotting against ISG15 revealed a smear of ISGylated proteins associated with LDs only when RNF213 was present. Immunoblotting against RNF213 confirmed knockdown of RNF213, while PLIN1, PLIN2, ATGL and GAPDH validated LD isolation and equal protein loading in the lysate and the LD- enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded).
Primary Human Cd14 + Monocytes, supplied by Voden Medical Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+monocytes/primary+human+cd14+++monocytes/10__1096_slash_fj__201901239r-25-0-15
Average 90 stars, based on 1 article reviews
primary human cd14 + monocytes - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
primary human monocyte-derived macrophages (mdm)  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare primary human monocyte-derived macrophages (mdm)
(A) FLAG immunoprecipitation was performed from lysates of HEK293T cells expressing FLAG-RNF213 or FLAG-eGFP in combination with HA-ISG15(AA) and the ISGylation machinery (E1, E2, E3). A smear of ISGylated co-immunoprecipitated proteins was detected with FLAG-RNF213, but not with FLAG-eGFP or when non-conjugatable HA-ISG15AA was used. (B) . THP-1 or primary human monocytes <t>(CD14+)</t> cells were cultured in the presence of 10 mM BSA-conjugated oleic acid and either treated with 10 ng/mL interferon-β for 8 h or left untreated. Lipid droplets (LDs)-enriched fractions were isolated by ultracentrifugation floatation assay on a sucrose step-gradient. Immunoblots against RNF213 and ISG15 revealed an interferon-induced upregulation of both proteins on LDs and a smear of ISGylated proteins associated with LDs. Immunoblots against PLIN1, PLIN2, ATGL and GAPDH confirmed LD isolation and equal protein loading in the lysate and LD-enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded). (C) Similarly, LDs were isolated from THP1 cells after knockdown of RNF213 by siRNA treatment for 48h or using a non-targeting scrambled siRNA (siScramble) as control. Immunoblotting against ISG15 revealed a smear of ISGylated proteins associated with LDs only when RNF213 was present. Immunoblotting against RNF213 confirmed knockdown of RNF213, while PLIN1, PLIN2, ATGL and GAPDH validated LD isolation and equal protein loading in the lysate and the LD- enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded).
Primary Human Monocyte Derived Macrophages (Mdm), supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+monocytes/primary+human+monocyte+derived+macrophages++mdm+/pmc06310093-42-2-20
Average 90 stars, based on 1 article reviews
primary human monocyte-derived macrophages (mdm) - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
human primary monocytes  (AllCells LLC)
90
AllCells LLC human primary monocytes
(A) FLAG immunoprecipitation was performed from lysates of HEK293T cells expressing FLAG-RNF213 or FLAG-eGFP in combination with HA-ISG15(AA) and the ISGylation machinery (E1, E2, E3). A smear of ISGylated co-immunoprecipitated proteins was detected with FLAG-RNF213, but not with FLAG-eGFP or when non-conjugatable HA-ISG15AA was used. (B) . THP-1 or primary human monocytes <t>(CD14+)</t> cells were cultured in the presence of 10 mM BSA-conjugated oleic acid and either treated with 10 ng/mL interferon-β for 8 h or left untreated. Lipid droplets (LDs)-enriched fractions were isolated by ultracentrifugation floatation assay on a sucrose step-gradient. Immunoblots against RNF213 and ISG15 revealed an interferon-induced upregulation of both proteins on LDs and a smear of ISGylated proteins associated with LDs. Immunoblots against PLIN1, PLIN2, ATGL and GAPDH confirmed LD isolation and equal protein loading in the lysate and LD-enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded). (C) Similarly, LDs were isolated from THP1 cells after knockdown of RNF213 by siRNA treatment for 48h or using a non-targeting scrambled siRNA (siScramble) as control. Immunoblotting against ISG15 revealed a smear of ISGylated proteins associated with LDs only when RNF213 was present. Immunoblotting against RNF213 confirmed knockdown of RNF213, while PLIN1, PLIN2, ATGL and GAPDH validated LD isolation and equal protein loading in the lysate and the LD- enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded).
Human Primary Monocytes, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+monocytes/human+primary+monocytes/pm19095472-62-0-12
Average 90 stars, based on 1 article reviews
human primary monocytes - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

Image Search Results


(A) FLAG immunoprecipitation was performed from lysates of HEK293T cells expressing FLAG-RNF213 or FLAG-eGFP in combination with HA-ISG15(AA) and the ISGylation machinery (E1, E2, E3). A smear of ISGylated co-immunoprecipitated proteins was detected with FLAG-RNF213, but not with FLAG-eGFP or when non-conjugatable HA-ISG15AA was used. (B) . THP-1 or primary human monocytes (CD14+) cells were cultured in the presence of 10 mM BSA-conjugated oleic acid and either treated with 10 ng/mL interferon-β for 8 h or left untreated. Lipid droplets (LDs)-enriched fractions were isolated by ultracentrifugation floatation assay on a sucrose step-gradient. Immunoblots against RNF213 and ISG15 revealed an interferon-induced upregulation of both proteins on LDs and a smear of ISGylated proteins associated with LDs. Immunoblots against PLIN1, PLIN2, ATGL and GAPDH confirmed LD isolation and equal protein loading in the lysate and LD-enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded). (C) Similarly, LDs were isolated from THP1 cells after knockdown of RNF213 by siRNA treatment for 48h or using a non-targeting scrambled siRNA (siScramble) as control. Immunoblotting against ISG15 revealed a smear of ISGylated proteins associated with LDs only when RNF213 was present. Immunoblotting against RNF213 confirmed knockdown of RNF213, while PLIN1, PLIN2, ATGL and GAPDH validated LD isolation and equal protein loading in the lysate and the LD- enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded).

Journal: bioRxiv

Article Title: Ring Finger Protein 213 Assembles into a Sensor for ISGylated Proteins with Antimicrobial Activity

doi: 10.1101/2021.06.03.446796

Figure Lengend Snippet: (A) FLAG immunoprecipitation was performed from lysates of HEK293T cells expressing FLAG-RNF213 or FLAG-eGFP in combination with HA-ISG15(AA) and the ISGylation machinery (E1, E2, E3). A smear of ISGylated co-immunoprecipitated proteins was detected with FLAG-RNF213, but not with FLAG-eGFP or when non-conjugatable HA-ISG15AA was used. (B) . THP-1 or primary human monocytes (CD14+) cells were cultured in the presence of 10 mM BSA-conjugated oleic acid and either treated with 10 ng/mL interferon-β for 8 h or left untreated. Lipid droplets (LDs)-enriched fractions were isolated by ultracentrifugation floatation assay on a sucrose step-gradient. Immunoblots against RNF213 and ISG15 revealed an interferon-induced upregulation of both proteins on LDs and a smear of ISGylated proteins associated with LDs. Immunoblots against PLIN1, PLIN2, ATGL and GAPDH confirmed LD isolation and equal protein loading in the lysate and LD-enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded). (C) Similarly, LDs were isolated from THP1 cells after knockdown of RNF213 by siRNA treatment for 48h or using a non-targeting scrambled siRNA (siScramble) as control. Immunoblotting against ISG15 revealed a smear of ISGylated proteins associated with LDs only when RNF213 was present. Immunoblotting against RNF213 confirmed knockdown of RNF213, while PLIN1, PLIN2, ATGL and GAPDH validated LD isolation and equal protein loading in the lysate and the LD- enriched fraction (1/20 th of the lysate and all of the LD-enriched material was loaded).

Article Snippet: Hek293T cells originate from (Lin et al., 2014) while HeLa cells (ATCC® CCL-2TM), A549 cells (ATCC® CCL-185TM) THP-1 cells (ATCC® TIB-202TM) and primary human CD14+ monocytes (ATCC® PCS- 800-010 TM ) were purchased from ATCC.

Techniques: Immunoprecipitation, Expressing, Cell Culture, Isolation, Western Blot, Knockdown, Control