Journal: Lab on a Chip

Article Title: Multiplexed fluidic circuit board for controlled perfusion of 3D blood vessels-on-a-chip †

doi: 10.1039/d2lc00686c

Figure Lengend Snippet: Design of the microfluidic system (a) the round microfluidic devices contain 3 microfluidic channels: (i) microfluidic channels are 1.1 cm long and 500 × 500 μm (wxh); (ii) VFP consists of injection of a viscous collagen mixture (pink) into the channel followed by a droplet of PBS; (iii) pipette-tips are removed and cells can be seeded. (b) Expanded view of the fluidic circuit board, which consists of a fluidic circuit top-layer, a connection layer housing the luer-to-1/16′ barb connectors to four individual devices. (c) The fluidic circuit contains two medium reservoirs (R1,R2) connected to pressure controllers (not shown), a feeder channel (green), τ EQ-resistors (yellow) and a waste channel (red) check valves are located at the medium reservoirs so recirculation can be achieved by switching high- and low pressure between the reservoirs. (d) 2p-SHG image shows the collagen fibrillar structure that reveals the lumen. (e) 3D reconstruction of TUBA1B-eGFP-ECs used for this study. (f) Photograph of the tested fluidic circuit board, connected to the external medium reservoirs in a heat block. Scalebar 100 μm.

Article Snippet: A custom python-based PID-controller was developed to control two 345 mbar LineUp EZ-flow (Fluigent) pneumatic pressure controllers.

Techniques: Injection, Transferring, Blocking Assay