preparative hplc Search Results


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  • 94
    Waters Corporation preparative hplc
    Purification of stingray ghrelin-like peptide (GRLN-LP) from stomach extracts . Black bars indicate the measured fluorescence changes in intracellular calcium ion concentrations in CHO cells expressing rat GHS-R1a (CHO-GHSR62). (A) Carboxymethyl (CM)-cation ion-exchange <t>HPLC</t> (pH 4.8) of the SP-III fraction of stomach extracts. The GRLN-like activity was divided into four groups (A-D). (B) Preparative reverse-phase (RP)-HPLC (Symmetry <t>C18,</t> 3.9 × 150 mm) of group B after purification with an anti-rat GRLN1-11 immuno-affinity column. (C) Final purification of the active fraction indicated in (B) by another RP-HPLC (Vydac diphenyl, 219TP5215, 2.1 × 150 mm).
    Preparative Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 2149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    JASCO Inc hplc system
    Purification of stingray ghrelin-like peptide (GRLN-LP) from stomach extracts . Black bars indicate the measured fluorescence changes in intracellular calcium ion concentrations in CHO cells expressing rat GHS-R1a (CHO-GHSR62). (A) Carboxymethyl (CM)-cation ion-exchange <t>HPLC</t> (pH 4.8) of the SP-III fraction of stomach extracts. The GRLN-like activity was divided into four groups (A-D). (B) Preparative reverse-phase (RP)-HPLC (Symmetry <t>C18,</t> 3.9 × 150 mm) of group B after purification with an anti-rat GRLN1-11 immuno-affinity column. (C) Final purification of the active fraction indicated in (B) by another RP-HPLC (Vydac diphenyl, 219TP5215, 2.1 × 150 mm).
    Hplc System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 99/100, based on 2093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Shimadzu Corporation preparative hplc
    <t>HPLC</t> chromatogram of black gram milled by-product fractions showing <t>vitexin</t> (1) and isovitexin (2). (A) Whole gram; (B) Dhal; (C) Germ; (D) Aleurone; (E) Plumule; (F) Husk.
    Preparative Hplc, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preparative hplc/product/Shimadzu Corporation
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    94
    Agilent technologies preparative hplc
    Maladicin biosynthesis, heterologous expression and structure (a) The <t>malacidin</t> biosynthetic gene cluster was recovered on three overlapping cosmid clones and (b) assembled from these three overlapping clones in yeast using transformation-associated recombination (TAR). The resulting bacterial artificial chromosome (BAC) was integrated into S. albus genome for heterologous expression studies. (c) A representative <t>HPLC</t> analysis of crude extracts derived from cultures of S. albus transformed with the malacidin biosynthetic gene cluster (BGC) shows the presence of BGC-specific small molecules. The two primary malacidin peaks are highlighted with an asterisk. (d) Unlike crude extracts of the S. albus host strain alone, only extracts from the S. albus harboring the malacidin BGC showed antibacterial activity when applied to a lawn of S. aureus USA300. Both the (c) HPLC analysis and (d) antibacterial activity are representative of 4 independent fermentations. (e) Malacidin A and B are cyclic lipopeptides containing 8 amino acid macrocycles and polyunsaturated lipids. The malacidins do not contain the conserved DXDG motif seen in all known calcium-dependent antibiotics – incorporating a rare 3-hydroxyl aspartic acid (HyAsp, highlighted in violet) and lacking the spacer residue. Biosynthetic enzymes predicted to be involved in the production of non-proteinogenic amino acid [3-methyl aspartic acid (MeAsp), 4-methyl proline (MePro), and 2,3-diamino 3-methyl propanoic acid (MeDap)] and fatty acid substrates required for the biosynthesis of the maladicins are shown and colored coded according to their activities. Stereocenters in malacidin that were predicted bioinformatically as opposed to through chemical and spectroscopic analysis are denoted with an asterisk.
    Preparative Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 1330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies semi preparative hplc
    <t>HPLC</t> analysis of a) Sep-Pak purified [ 18 F]c(RGDfK); b) HPLC purified [ 18 F]c(RGDfK); c) [ 18 F]c(RGDfK), co-injected with the non-radioactive standard. HPLC condition: <t>Agilent</t> Eclipse plus C18 column (4.6 × 150 mm, 3.5 μm), mobile phase: 10 - 50% A in 8 min, 50 – 90 % A in 15 min. A = acetonitrile (0.1% TFA), B = water (0.1% TFA), with a flow rate of 1.0 mL/min. Solid line, in-line radiodetector; dotted line, UV detector at 254 nm.
    Semi Preparative Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 1151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Phenomenex preparative hplc
    <t>HPLC</t> chromatograms of the crude 5-[ 18 F]FDR-AOA-peptide conjugates prepared using ammonium acetate (( A , C , E ); reaction conditions I) and anilinium acetate (( B , D , F , H ); reaction conditions II) as well as purified 5-[ 18 F]FDR-AOA-Clone 27 ([ 18 F] 11 , ( G )). Reaction conditions: I: 0.2 mol·L −1 NH 4 OAc buffer, pH 4, 75 °C, 20 min; II: 0.25 mol·L −1 anilinium acetate buffer, pH 4.6, RT, 10 min, for the applied amounts of AOA-peptides, refer to Table 2 and Table 3 . HPLC conditions: column: <t>Phenomenex</t> Kinetex C18 100 × 4.6 mm, 2.6 μm/100 Å; eluent: 0–15 min 10 → 40% MeCN (0.1% TFA); flow rate: 1 mL/min; t r : [ 18 F]FDR-AOA-C-CPE-17-KKK ([ 18 F] 8 , ( A , B ))—11.5 min, [ 18 F]FDR-AOA-CC4P-5 ([ 18 F] 10 , ( C , D ))—10.3 min, [ 18 F]FDR-AOA-Clone 27 ([ 18 F] 11 , ( E , F ))—7.5 min, [ 18 F]FDR-AOA-M19 ([ 18 F] 9 , ( H ))—11.6 min, side product: 5-[ 18 F]FDR—1.4 min. Radioactivity and UV- (λ = 210 nm; only ( G )) traces are shown in black and violet, respectively.
    Preparative Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 94/100, based on 1161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Phenomenex semi preparative hplc
    <t>RPIP-HPLC-MS</t> total ion chromatograms (TICs) of reaction products from the sulfonation of N <t>-cbz</t> aminoglycosides. Shown are product mixtures from the sulfonation of kanamycin and apramycin with Pyr·SO 3 (A and B, respectively) and the sulfonation
    Semi Preparative Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 92/100, based on 1032 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Waters Corporation semi preparative hplc
    In vitro stability of Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1 ) at physiological pH was confirmed by <t>HPLC</t> at 2 hours ( A ) and 23 hours ( B ) after radiolabeling. The upper trace shows the radio-profile (red) and the lower trace shows the UV profile at 254 nm (green).(HPLC: 0.05 M TEAP pH 7/MeOH, 80:20 v/v, isocratic method, flow 1 mL/min).
    Semi Preparative Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 920 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Shimadzu Corporation semi preparative hplc
    Engineered synthesis of 4-hydroxy-6-isovaleryl-2-pyrone <t>(HIBP)</t> and phlorisovalerophenone (PIVP) by E. coli strains. a , b Strain APG-0 harboring empty vectors as negative control. c , d Strain APG-1 harboring plasmids pET-A and pCDF-EV1. e , f Strain APG-2 harboring plasmids pET-A and pCDF-EV2. g , h Strain APG-3 harboring plasmids pET-A and pCDF-EV3. a , c , e , g <t>HPLC</t> analysis of metabolites extracted from cell pellets. b , d , f , h HPLC analysis of metabolites extracted from the fermentation broth. i The titers of HIBP produced by the strains APG-1, APG-2 and APG-3. j The titers of PIVP produced by the strains APG-1, APG-2 and APG-3
    Semi Preparative Hplc, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 92/100, based on 501 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Phenomenex preparative reversed phase hplc
    Engineered synthesis of 4-hydroxy-6-isovaleryl-2-pyrone <t>(HIBP)</t> and phlorisovalerophenone (PIVP) by E. coli strains. a , b Strain APG-0 harboring empty vectors as negative control. c , d Strain APG-1 harboring plasmids pET-A and pCDF-EV1. e , f Strain APG-2 harboring plasmids pET-A and pCDF-EV2. g , h Strain APG-3 harboring plasmids pET-A and pCDF-EV3. a , c , e , g <t>HPLC</t> analysis of metabolites extracted from cell pellets. b , d , f , h HPLC analysis of metabolites extracted from the fermentation broth. i The titers of HIBP produced by the strains APG-1, APG-2 and APG-3. j The titers of PIVP produced by the strains APG-1, APG-2 and APG-3
    Preparative Reversed Phase Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 91/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Gilson Inc preparative hplc
    Engineered synthesis of 4-hydroxy-6-isovaleryl-2-pyrone <t>(HIBP)</t> and phlorisovalerophenone (PIVP) by E. coli strains. a , b Strain APG-0 harboring empty vectors as negative control. c , d Strain APG-1 harboring plasmids pET-A and pCDF-EV1. e , f Strain APG-2 harboring plasmids pET-A and pCDF-EV2. g , h Strain APG-3 harboring plasmids pET-A and pCDF-EV3. a , c , e , g <t>HPLC</t> analysis of metabolites extracted from cell pellets. b , d , f , h HPLC analysis of metabolites extracted from the fermentation broth. i The titers of HIBP produced by the strains APG-1, APG-2 and APG-3. j The titers of PIVP produced by the strains APG-1, APG-2 and APG-3
    Preparative Hplc, supplied by Gilson Inc, used in various techniques. Bioz Stars score: 92/100, based on 449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Phenomenex semi preparative hplc column
    Engineered synthesis of 4-hydroxy-6-isovaleryl-2-pyrone <t>(HIBP)</t> and phlorisovalerophenone (PIVP) by E. coli strains. a , b Strain APG-0 harboring empty vectors as negative control. c , d Strain APG-1 harboring plasmids pET-A and pCDF-EV1. e , f Strain APG-2 harboring plasmids pET-A and pCDF-EV2. g , h Strain APG-3 harboring plasmids pET-A and pCDF-EV3. a , c , e , g <t>HPLC</t> analysis of metabolites extracted from cell pellets. b , d , f , h HPLC analysis of metabolites extracted from the fermentation broth. i The titers of HIBP produced by the strains APG-1, APG-2 and APG-3. j The titers of PIVP produced by the strains APG-1, APG-2 and APG-3
    Semi Preparative Hplc Column, supplied by Phenomenex, used in various techniques. Bioz Stars score: 89/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Hitachi Ltd semi preparative hplc
    Engineered synthesis of 4-hydroxy-6-isovaleryl-2-pyrone <t>(HIBP)</t> and phlorisovalerophenone (PIVP) by E. coli strains. a , b Strain APG-0 harboring empty vectors as negative control. c , d Strain APG-1 harboring plasmids pET-A and pCDF-EV1. e , f Strain APG-2 harboring plasmids pET-A and pCDF-EV2. g , h Strain APG-3 harboring plasmids pET-A and pCDF-EV3. a , c , e , g <t>HPLC</t> analysis of metabolites extracted from cell pellets. b , d , f , h HPLC analysis of metabolites extracted from the fermentation broth. i The titers of HIBP produced by the strains APG-1, APG-2 and APG-3. j The titers of PIVP produced by the strains APG-1, APG-2 and APG-3
    Semi Preparative Hplc, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 92/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Phenomenex preparative reverse phase hplc
    Engineered synthesis of 4-hydroxy-6-isovaleryl-2-pyrone <t>(HIBP)</t> and phlorisovalerophenone (PIVP) by E. coli strains. a , b Strain APG-0 harboring empty vectors as negative control. c , d Strain APG-1 harboring plasmids pET-A and pCDF-EV1. e , f Strain APG-2 harboring plasmids pET-A and pCDF-EV2. g , h Strain APG-3 harboring plasmids pET-A and pCDF-EV3. a , c , e , g <t>HPLC</t> analysis of metabolites extracted from cell pellets. b , d , f , h HPLC analysis of metabolites extracted from the fermentation broth. i The titers of HIBP produced by the strains APG-1, APG-2 and APG-3. j The titers of PIVP produced by the strains APG-1, APG-2 and APG-3
    Preparative Reverse Phase Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 91/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    MACHEREY NAGEL preparative hplc
    Engineered synthesis of 4-hydroxy-6-isovaleryl-2-pyrone <t>(HIBP)</t> and phlorisovalerophenone (PIVP) by E. coli strains. a , b Strain APG-0 harboring empty vectors as negative control. c , d Strain APG-1 harboring plasmids pET-A and pCDF-EV1. e , f Strain APG-2 harboring plasmids pET-A and pCDF-EV2. g , h Strain APG-3 harboring plasmids pET-A and pCDF-EV3. a , c , e , g <t>HPLC</t> analysis of metabolites extracted from cell pellets. b , d , f , h HPLC analysis of metabolites extracted from the fermentation broth. i The titers of HIBP produced by the strains APG-1, APG-2 and APG-3. j The titers of PIVP produced by the strains APG-1, APG-2 and APG-3
    Preparative Hplc, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Purification of stingray ghrelin-like peptide (GRLN-LP) from stomach extracts . Black bars indicate the measured fluorescence changes in intracellular calcium ion concentrations in CHO cells expressing rat GHS-R1a (CHO-GHSR62). (A) Carboxymethyl (CM)-cation ion-exchange HPLC (pH 4.8) of the SP-III fraction of stomach extracts. The GRLN-like activity was divided into four groups (A-D). (B) Preparative reverse-phase (RP)-HPLC (Symmetry C18, 3.9 × 150 mm) of group B after purification with an anti-rat GRLN1-11 immuno-affinity column. (C) Final purification of the active fraction indicated in (B) by another RP-HPLC (Vydac diphenyl, 219TP5215, 2.1 × 150 mm).

    Journal: BMC Biochemistry

    Article Title: Ghrelin-like peptide with fatty acid modification and O-glycosylation in the red stingray, Dasyatis akajei

    doi: 10.1186/1471-2091-10-30

    Figure Lengend Snippet: Purification of stingray ghrelin-like peptide (GRLN-LP) from stomach extracts . Black bars indicate the measured fluorescence changes in intracellular calcium ion concentrations in CHO cells expressing rat GHS-R1a (CHO-GHSR62). (A) Carboxymethyl (CM)-cation ion-exchange HPLC (pH 4.8) of the SP-III fraction of stomach extracts. The GRLN-like activity was divided into four groups (A-D). (B) Preparative reverse-phase (RP)-HPLC (Symmetry C18, 3.9 × 150 mm) of group B after purification with an anti-rat GRLN1-11 immuno-affinity column. (C) Final purification of the active fraction indicated in (B) by another RP-HPLC (Vydac diphenyl, 219TP5215, 2.1 × 150 mm).

    Article Snippet: The samples were first applied to a preparative RP-HPLC with a Symmetry C18 column (3.9 × 150 mm, Waters) at a flow rate of 1 ml/min under a linear gradient from 10% to 60% acetonitlile containing 0.1% TFA for 40 min.

    Techniques: Purification, Fluorescence, Expressing, High Performance Liquid Chromatography, Activity Assay, Affinity Column

    HPLC chromatogram of black gram milled by-product fractions showing vitexin (1) and isovitexin (2). (A) Whole gram; (B) Dhal; (C) Germ; (D) Aleurone; (E) Plumule; (F) Husk.

    Journal: Toxicology Reports

    Article Title: C-Glycosylated flavonoids from black gram husk: Protection against DNA and erythrocytes from oxidative damage and their cytotoxic effect on HeLa cells

    doi: 10.1016/j.toxrep.2016.08.006

    Figure Lengend Snippet: HPLC chromatogram of black gram milled by-product fractions showing vitexin (1) and isovitexin (2). (A) Whole gram; (B) Dhal; (C) Germ; (D) Aleurone; (E) Plumule; (F) Husk.

    Article Snippet: 2.5 Purification of vitexin and isovitexin of aqueous ethanol fraction by preparative HPLC The chromatographic separation was performed according to the method described in Section , on a Shimadzu Prep LC8A Preparative Chromatography system equipped with SCL-10AVP system controller (Shimadzu).

    Techniques: High Performance Liquid Chromatography

    RP-HPLC chromatograms of flavone C -glycosides purified from husk extract. (A) vitexin (B) isovitexin.

    Journal: Toxicology Reports

    Article Title: C-Glycosylated flavonoids from black gram husk: Protection against DNA and erythrocytes from oxidative damage and their cytotoxic effect on HeLa cells

    doi: 10.1016/j.toxrep.2016.08.006

    Figure Lengend Snippet: RP-HPLC chromatograms of flavone C -glycosides purified from husk extract. (A) vitexin (B) isovitexin.

    Article Snippet: 2.5 Purification of vitexin and isovitexin of aqueous ethanol fraction by preparative HPLC The chromatographic separation was performed according to the method described in Section , on a Shimadzu Prep LC8A Preparative Chromatography system equipped with SCL-10AVP system controller (Shimadzu).

    Techniques: High Performance Liquid Chromatography, Purification

    Maladicin biosynthesis, heterologous expression and structure (a) The malacidin biosynthetic gene cluster was recovered on three overlapping cosmid clones and (b) assembled from these three overlapping clones in yeast using transformation-associated recombination (TAR). The resulting bacterial artificial chromosome (BAC) was integrated into S. albus genome for heterologous expression studies. (c) A representative HPLC analysis of crude extracts derived from cultures of S. albus transformed with the malacidin biosynthetic gene cluster (BGC) shows the presence of BGC-specific small molecules. The two primary malacidin peaks are highlighted with an asterisk. (d) Unlike crude extracts of the S. albus host strain alone, only extracts from the S. albus harboring the malacidin BGC showed antibacterial activity when applied to a lawn of S. aureus USA300. Both the (c) HPLC analysis and (d) antibacterial activity are representative of 4 independent fermentations. (e) Malacidin A and B are cyclic lipopeptides containing 8 amino acid macrocycles and polyunsaturated lipids. The malacidins do not contain the conserved DXDG motif seen in all known calcium-dependent antibiotics – incorporating a rare 3-hydroxyl aspartic acid (HyAsp, highlighted in violet) and lacking the spacer residue. Biosynthetic enzymes predicted to be involved in the production of non-proteinogenic amino acid [3-methyl aspartic acid (MeAsp), 4-methyl proline (MePro), and 2,3-diamino 3-methyl propanoic acid (MeDap)] and fatty acid substrates required for the biosynthesis of the maladicins are shown and colored coded according to their activities. Stereocenters in malacidin that were predicted bioinformatically as opposed to through chemical and spectroscopic analysis are denoted with an asterisk.

    Journal: Nature microbiology

    Article Title: Culture-independent discovery of the malacidins as calcium-dependent antibiotics with activity against multidrug-resistant Gram-positive pathogens

    doi: 10.1038/s41564-018-0110-1

    Figure Lengend Snippet: Maladicin biosynthesis, heterologous expression and structure (a) The malacidin biosynthetic gene cluster was recovered on three overlapping cosmid clones and (b) assembled from these three overlapping clones in yeast using transformation-associated recombination (TAR). The resulting bacterial artificial chromosome (BAC) was integrated into S. albus genome for heterologous expression studies. (c) A representative HPLC analysis of crude extracts derived from cultures of S. albus transformed with the malacidin biosynthetic gene cluster (BGC) shows the presence of BGC-specific small molecules. The two primary malacidin peaks are highlighted with an asterisk. (d) Unlike crude extracts of the S. albus host strain alone, only extracts from the S. albus harboring the malacidin BGC showed antibacterial activity when applied to a lawn of S. aureus USA300. Both the (c) HPLC analysis and (d) antibacterial activity are representative of 4 independent fermentations. (e) Malacidin A and B are cyclic lipopeptides containing 8 amino acid macrocycles and polyunsaturated lipids. The malacidins do not contain the conserved DXDG motif seen in all known calcium-dependent antibiotics – incorporating a rare 3-hydroxyl aspartic acid (HyAsp, highlighted in violet) and lacking the spacer residue. Biosynthetic enzymes predicted to be involved in the production of non-proteinogenic amino acid [3-methyl aspartic acid (MeAsp), 4-methyl proline (MePro), and 2,3-diamino 3-methyl propanoic acid (MeDap)] and fatty acid substrates required for the biosynthesis of the maladicins are shown and colored coded according to their activities. Stereocenters in malacidin that were predicted bioinformatically as opposed to through chemical and spectroscopic analysis are denoted with an asterisk.

    Article Snippet: Combined fractions containing either malacidin A or B were subsequently cleaned up individually using preparative HPLC (XBridge Prep C18, 10 × 150 mM, 5 μM, Agilent HPLC System) using a linear gradient of 0.1 % trifloroacetic acid-acetonitrile from 30% to 50% over 30 min at a flow rate of 4 mL min−1 .

    Techniques: Expressing, Clone Assay, Transformation Assay, BAC Assay, High Performance Liquid Chromatography, Derivative Assay, Activity Assay

    HPLC analysis of a) Sep-Pak purified [ 18 F]c(RGDfK); b) HPLC purified [ 18 F]c(RGDfK); c) [ 18 F]c(RGDfK), co-injected with the non-radioactive standard. HPLC condition: Agilent Eclipse plus C18 column (4.6 × 150 mm, 3.5 μm), mobile phase: 10 - 50% A in 8 min, 50 – 90 % A in 15 min. A = acetonitrile (0.1% TFA), B = water (0.1% TFA), with a flow rate of 1.0 mL/min. Solid line, in-line radiodetector; dotted line, UV detector at 254 nm.

    Journal: Journal of labelled compounds & radiopharmaceuticals

    Article Title: Fast Indirect Fluorine-18 Labeling of Protein/Peptide using the useful 6-Fluoronicotinic acid-2,3,5,6-Tetrafluorophenyl prosthetic group: A Method Comparable to direct Fluorination

    doi: 10.1002/jlcr.3487

    Figure Lengend Snippet: HPLC analysis of a) Sep-Pak purified [ 18 F]c(RGDfK); b) HPLC purified [ 18 F]c(RGDfK); c) [ 18 F]c(RGDfK), co-injected with the non-radioactive standard. HPLC condition: Agilent Eclipse plus C18 column (4.6 × 150 mm, 3.5 μm), mobile phase: 10 - 50% A in 8 min, 50 – 90 % A in 15 min. A = acetonitrile (0.1% TFA), B = water (0.1% TFA), with a flow rate of 1.0 mL/min. Solid line, in-line radiodetector; dotted line, UV detector at 254 nm.

    Article Snippet: The reaction mixture was stirred at 50 °C for 1 h. The product was purified by semi-preparative HPLC (conditions: Agilent Eclipse plus C18 column (9.4 × 250 mm, 5 μm), mobile phase: 5 - 50% acetonitrile in water (0.1% TFA), with a flow rate of 4.0 mL/min.)

    Techniques: High Performance Liquid Chromatography, Purification, Injection, Flow Cytometry

    HPLC chromatograms of the crude 5-[ 18 F]FDR-AOA-peptide conjugates prepared using ammonium acetate (( A , C , E ); reaction conditions I) and anilinium acetate (( B , D , F , H ); reaction conditions II) as well as purified 5-[ 18 F]FDR-AOA-Clone 27 ([ 18 F] 11 , ( G )). Reaction conditions: I: 0.2 mol·L −1 NH 4 OAc buffer, pH 4, 75 °C, 20 min; II: 0.25 mol·L −1 anilinium acetate buffer, pH 4.6, RT, 10 min, for the applied amounts of AOA-peptides, refer to Table 2 and Table 3 . HPLC conditions: column: Phenomenex Kinetex C18 100 × 4.6 mm, 2.6 μm/100 Å; eluent: 0–15 min 10 → 40% MeCN (0.1% TFA); flow rate: 1 mL/min; t r : [ 18 F]FDR-AOA-C-CPE-17-KKK ([ 18 F] 8 , ( A , B ))—11.5 min, [ 18 F]FDR-AOA-CC4P-5 ([ 18 F] 10 , ( C , D ))—10.3 min, [ 18 F]FDR-AOA-Clone 27 ([ 18 F] 11 , ( E , F ))—7.5 min, [ 18 F]FDR-AOA-M19 ([ 18 F] 9 , ( H ))—11.6 min, side product: 5-[ 18 F]FDR—1.4 min. Radioactivity and UV- (λ = 210 nm; only ( G )) traces are shown in black and violet, respectively.

    Journal: Pharmaceuticals

    Article Title: Convenient Preparation of 18F-Labeled Peptide Probes for Potential Claudin-4 PET Imaging

    doi: 10.3390/ph10040099

    Figure Lengend Snippet: HPLC chromatograms of the crude 5-[ 18 F]FDR-AOA-peptide conjugates prepared using ammonium acetate (( A , C , E ); reaction conditions I) and anilinium acetate (( B , D , F , H ); reaction conditions II) as well as purified 5-[ 18 F]FDR-AOA-Clone 27 ([ 18 F] 11 , ( G )). Reaction conditions: I: 0.2 mol·L −1 NH 4 OAc buffer, pH 4, 75 °C, 20 min; II: 0.25 mol·L −1 anilinium acetate buffer, pH 4.6, RT, 10 min, for the applied amounts of AOA-peptides, refer to Table 2 and Table 3 . HPLC conditions: column: Phenomenex Kinetex C18 100 × 4.6 mm, 2.6 μm/100 Å; eluent: 0–15 min 10 → 40% MeCN (0.1% TFA); flow rate: 1 mL/min; t r : [ 18 F]FDR-AOA-C-CPE-17-KKK ([ 18 F] 8 , ( A , B ))—11.5 min, [ 18 F]FDR-AOA-CC4P-5 ([ 18 F] 10 , ( C , D ))—10.3 min, [ 18 F]FDR-AOA-Clone 27 ([ 18 F] 11 , ( E , F ))—7.5 min, [ 18 F]FDR-AOA-M19 ([ 18 F] 9 , ( H ))—11.6 min, side product: 5-[ 18 F]FDR—1.4 min. Radioactivity and UV- (λ = 210 nm; only ( G )) traces are shown in black and violet, respectively.

    Article Snippet: Further purification of the peptides was achieved by preparative HPLC on RP18 Phenomenex column (Jupiter Proteo, 250 × 15 mm, 4 μm/90 Å) using linear gradients of 10–60% B in A (A = 0.1% TFA in water; B = 0.1% TFA in acetonitrile) over 45 min and a flow rate of 6 mL min−1 .

    Techniques: High Performance Liquid Chromatography, Purification, Flow Cytometry, Radioactivity

    HPLC chromatograms of the crude and purified [ 18 F] 6 (( A , B ), respectively), and 5-[ 18 F]FDR ([ 18 F] 7 , ( C )). Conditions: column: Phenomenex Luna C18(2), 250 × 4.6 mm, 5 µm; eluent: 0–5.5 min 55 → 95% MeCN; 5.5–8.0 min 95% MeCN; flow rate: 1.3 mL/min; t r = 4.5 min ([ 18 F] 6 ), t r = 2.0 min (5-[ 18 F]FDR). Radioactivity, UV- (λ = 210 nm), and ELSD-traces are shown in black, violet, and green, respectively.

    Journal: Pharmaceuticals

    Article Title: Convenient Preparation of 18F-Labeled Peptide Probes for Potential Claudin-4 PET Imaging

    doi: 10.3390/ph10040099

    Figure Lengend Snippet: HPLC chromatograms of the crude and purified [ 18 F] 6 (( A , B ), respectively), and 5-[ 18 F]FDR ([ 18 F] 7 , ( C )). Conditions: column: Phenomenex Luna C18(2), 250 × 4.6 mm, 5 µm; eluent: 0–5.5 min 55 → 95% MeCN; 5.5–8.0 min 95% MeCN; flow rate: 1.3 mL/min; t r = 4.5 min ([ 18 F] 6 ), t r = 2.0 min (5-[ 18 F]FDR). Radioactivity, UV- (λ = 210 nm), and ELSD-traces are shown in black, violet, and green, respectively.

    Article Snippet: Further purification of the peptides was achieved by preparative HPLC on RP18 Phenomenex column (Jupiter Proteo, 250 × 15 mm, 4 μm/90 Å) using linear gradients of 10–60% B in A (A = 0.1% TFA in water; B = 0.1% TFA in acetonitrile) over 45 min and a flow rate of 6 mL min−1 .

    Techniques: High Performance Liquid Chromatography, Purification, Flow Cytometry, Radioactivity

    RPIP-HPLC-MS total ion chromatograms (TICs) of reaction products from the sulfonation of N -cbz aminoglycosides. Shown are product mixtures from the sulfonation of kanamycin and apramycin with Pyr·SO 3 (A and B, respectively) and the sulfonation

    Journal: Carbohydrate research

    Article Title: Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods

    doi: 10.1016/j.carres.2011.09.020

    Figure Lengend Snippet: RPIP-HPLC-MS total ion chromatograms (TICs) of reaction products from the sulfonation of N -cbz aminoglycosides. Shown are product mixtures from the sulfonation of kanamycin and apramycin with Pyr·SO 3 (A and B, respectively) and the sulfonation

    Article Snippet: In some reactions, over-acylated product (having at least one O -cbz) was detected by ESI-MS; the desired product was then purified using semi-preparative HPLC.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    Representative comparison of analytical HPLC chromatograms from various RPIP conditions eluting persulfated N -cbz neomycin. Each chromatogram was generated from a 20 min. gradient increase in percent ACN, and the ending percent ACN was then maintained

    Journal: Carbohydrate research

    Article Title: Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods

    doi: 10.1016/j.carres.2011.09.020

    Figure Lengend Snippet: Representative comparison of analytical HPLC chromatograms from various RPIP conditions eluting persulfated N -cbz neomycin. Each chromatogram was generated from a 20 min. gradient increase in percent ACN, and the ending percent ACN was then maintained

    Article Snippet: In some reactions, over-acylated product (having at least one O -cbz) was detected by ESI-MS; the desired product was then purified using semi-preparative HPLC.

    Techniques: High Performance Liquid Chromatography, Generated

    Adaptation of RPIP-HPLC method developed here to separate sulfonated products from reaction mixtures by gravity flow benchtop C18 resin. N -cbz O -sulfonated neomycin was eluted in 10 mM ammonium acetate, initially in 100% water, and then with addition

    Journal: Carbohydrate research

    Article Title: Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods

    doi: 10.1016/j.carres.2011.09.020

    Figure Lengend Snippet: Adaptation of RPIP-HPLC method developed here to separate sulfonated products from reaction mixtures by gravity flow benchtop C18 resin. N -cbz O -sulfonated neomycin was eluted in 10 mM ammonium acetate, initially in 100% water, and then with addition

    Article Snippet: In some reactions, over-acylated product (having at least one O -cbz) was detected by ESI-MS; the desired product was then purified using semi-preparative HPLC.

    Techniques: High Performance Liquid Chromatography, Flow Cytometry

    Separation of N -cbz O -sulfonated kanamycin derivatives that differ by degree of sulfation and the position of sulfate groups using RPIP-HPLC interfaced with an ESI- ion trap mass analyzer. Peaks for all sulfonation products are shown in the total ion

    Journal: Carbohydrate research

    Article Title: Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods

    doi: 10.1016/j.carres.2011.09.020

    Figure Lengend Snippet: Separation of N -cbz O -sulfonated kanamycin derivatives that differ by degree of sulfation and the position of sulfate groups using RPIP-HPLC interfaced with an ESI- ion trap mass analyzer. Peaks for all sulfonation products are shown in the total ion

    Article Snippet: In some reactions, over-acylated product (having at least one O -cbz) was detected by ESI-MS; the desired product was then purified using semi-preparative HPLC.

    Techniques: High Performance Liquid Chromatography

    RPIP-HPLC to follow reaction progression over time for O -sulfonation of N -cbz neomycin. Following addition of ClSO 3 H, reaction aliquots were analyzed at: (A) 5 min, (B) 15 min, (C) 20 min, (D) 50 min, (E) 275 min. Percent of reaction product that is persulfated

    Journal: Carbohydrate research

    Article Title: Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods

    doi: 10.1016/j.carres.2011.09.020

    Figure Lengend Snippet: RPIP-HPLC to follow reaction progression over time for O -sulfonation of N -cbz neomycin. Following addition of ClSO 3 H, reaction aliquots were analyzed at: (A) 5 min, (B) 15 min, (C) 20 min, (D) 50 min, (E) 275 min. Percent of reaction product that is persulfated

    Article Snippet: In some reactions, over-acylated product (having at least one O -cbz) was detected by ESI-MS; the desired product was then purified using semi-preparative HPLC.

    Techniques: High Performance Liquid Chromatography

    Sulfonation of N -cbz aminoglycosides (kanamycin, apramycin, and neomycin) with Pyr·SO 3 or ClSO 3 H resulted in products with varied degrees of sulfation (DS). RPIP-HPLC methods developed here were used to separate reaction products based on degree

    Journal: Carbohydrate research

    Article Title: Synthesis, separation, and characterization of amphiphilic sulfated oligosaccharides enabled by reversed-phase ion pairing LC and LC-MS methods

    doi: 10.1016/j.carres.2011.09.020

    Figure Lengend Snippet: Sulfonation of N -cbz aminoglycosides (kanamycin, apramycin, and neomycin) with Pyr·SO 3 or ClSO 3 H resulted in products with varied degrees of sulfation (DS). RPIP-HPLC methods developed here were used to separate reaction products based on degree

    Article Snippet: In some reactions, over-acylated product (having at least one O -cbz) was detected by ESI-MS; the desired product was then purified using semi-preparative HPLC.

    Techniques: High Performance Liquid Chromatography

    Recombinant production of Mb1a. Semi-preparative RP-HPLC chromatogram of recombinant Mb1a released by TEV protease cleavage of the MBP-Mb1a fusion protein (see Materials and Methods for more details). The dotted line indicates the gradient of solvent B (90% acetonitrile/0.043% TFA). Top inset: SDS-PAGE gel showing pre-cleaved MBP-Mb1a fusion protein (lane 1) and remaining MBP after cleavage (lane 2). Lane M contains molecular markers (masses in kDa). Bottom inset: MALDI-TOF mass spectrum of pure recombinant Mb1a.

    Journal: Toxins

    Article Title: Insect-Active Toxins with Promiscuous Pharmacology from the African Theraphosid Spider Monocentropus balfouri

    doi: 10.3390/toxins9050155

    Figure Lengend Snippet: Recombinant production of Mb1a. Semi-preparative RP-HPLC chromatogram of recombinant Mb1a released by TEV protease cleavage of the MBP-Mb1a fusion protein (see Materials and Methods for more details). The dotted line indicates the gradient of solvent B (90% acetonitrile/0.043% TFA). Top inset: SDS-PAGE gel showing pre-cleaved MBP-Mb1a fusion protein (lane 1) and remaining MBP after cleavage (lane 2). Lane M contains molecular markers (masses in kDa). Bottom inset: MALDI-TOF mass spectrum of pure recombinant Mb1a.

    Article Snippet: The supernatant was filtered with a 0.45 μm syringe filter (EMD Millipore, Billerica, MA, USA) before purification of recombinant Mb1a or Mb1b using semi-preparative RP-HPLC (Phenomenex Jupiter C4 column; 250 × 10 mm, 10 μm; flow rate 5 mL/min).

    Techniques: Recombinant, High Performance Liquid Chromatography, SDS Page

    ( a ) Photo of a female M. balfouri ; ( b ) Chromatogram resulting from RP-HPLC fractionation of M. balfouri venom. The peak highlighted in red contains the µ/ω-TRTX-Mb1a/b peptide. The dotted line indicates the gradient of solvent B (90% acetonitrile/0.1% formic acid). Inset is a MALDI-TOF mass spectrum of the isolated µ/ω-TRTX-Mb1a/b peptide.

    Journal: Toxins

    Article Title: Insect-Active Toxins with Promiscuous Pharmacology from the African Theraphosid Spider Monocentropus balfouri

    doi: 10.3390/toxins9050155

    Figure Lengend Snippet: ( a ) Photo of a female M. balfouri ; ( b ) Chromatogram resulting from RP-HPLC fractionation of M. balfouri venom. The peak highlighted in red contains the µ/ω-TRTX-Mb1a/b peptide. The dotted line indicates the gradient of solvent B (90% acetonitrile/0.1% formic acid). Inset is a MALDI-TOF mass spectrum of the isolated µ/ω-TRTX-Mb1a/b peptide.

    Article Snippet: The supernatant was filtered with a 0.45 μm syringe filter (EMD Millipore, Billerica, MA, USA) before purification of recombinant Mb1a or Mb1b using semi-preparative RP-HPLC (Phenomenex Jupiter C4 column; 250 × 10 mm, 10 μm; flow rate 5 mL/min).

    Techniques: High Performance Liquid Chromatography, Fractionation, Isolation

    In vitro stability of Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1 ) at physiological pH was confirmed by HPLC at 2 hours ( A ) and 23 hours ( B ) after radiolabeling. The upper trace shows the radio-profile (red) and the lower trace shows the UV profile at 254 nm (green).(HPLC: 0.05 M TEAP pH 7/MeOH, 80:20 v/v, isocratic method, flow 1 mL/min).

    Journal: Nuclear medicine and biology

    Article Title: Re(CO)3([18F]FEDA), a novel 18F PET renal tracer: radiosynthesis and preclinical evaluation

    doi: 10.1016/j.nucmedbio.2017.12.001

    Figure Lengend Snippet: In vitro stability of Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1 ) at physiological pH was confirmed by HPLC at 2 hours ( A ) and 23 hours ( B ) after radiolabeling. The upper trace shows the radio-profile (red) and the lower trace shows the UV profile at 254 nm (green).(HPLC: 0.05 M TEAP pH 7/MeOH, 80:20 v/v, isocratic method, flow 1 mL/min).

    Article Snippet: The crude radiotracer Re(CO)3 ([18 F]FEDA) (18 F-1 ) was purified by semi-preparative HPLC and the desired pure radioactive fractions were combined.

    Techniques: In Vitro, High Performance Liquid Chromatography, Radioactivity, Flow Cytometry

    HPLC chromatograms of Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1 ; top, red) co-injected with Re(CO) 3 (FEDA) ( 1 ; bottom, green) to confirm the radiotracer identity (HPLC: 0.05 M TEAP pH 7/MeOH, 80:20 v/v, isocratic method, flow 1 mL/min).

    Journal: Nuclear medicine and biology

    Article Title: Re(CO)3([18F]FEDA), a novel 18F PET renal tracer: radiosynthesis and preclinical evaluation

    doi: 10.1016/j.nucmedbio.2017.12.001

    Figure Lengend Snippet: HPLC chromatograms of Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1 ; top, red) co-injected with Re(CO) 3 (FEDA) ( 1 ; bottom, green) to confirm the radiotracer identity (HPLC: 0.05 M TEAP pH 7/MeOH, 80:20 v/v, isocratic method, flow 1 mL/min).

    Article Snippet: The crude radiotracer Re(CO)3 ([18 F]FEDA) (18 F-1 ) was purified by semi-preparative HPLC and the desired pure radioactive fractions were combined.

    Techniques: High Performance Liquid Chromatography, Injection, Flow Cytometry

    Radio-HPLC chromatograms of purified Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1 ) before injection ( A ) and of rat urine 10 min after i.v. injection of ( 18 F-1 ) ( B ] flow 1 mL/min).

    Journal: Nuclear medicine and biology

    Article Title: Re(CO)3([18F]FEDA), a novel 18F PET renal tracer: radiosynthesis and preclinical evaluation

    doi: 10.1016/j.nucmedbio.2017.12.001

    Figure Lengend Snippet: Radio-HPLC chromatograms of purified Re(CO) 3 ([ 18 F]FEDA) ( 18 F-1 ) before injection ( A ) and of rat urine 10 min after i.v. injection of ( 18 F-1 ) ( B ] flow 1 mL/min).

    Article Snippet: The crude radiotracer Re(CO)3 ([18 F]FEDA) (18 F-1 ) was purified by semi-preparative HPLC and the desired pure radioactive fractions were combined.

    Techniques: High Performance Liquid Chromatography, Purification, Injection, Flow Cytometry

    Engineered synthesis of 4-hydroxy-6-isovaleryl-2-pyrone (HIBP) and phlorisovalerophenone (PIVP) by E. coli strains. a , b Strain APG-0 harboring empty vectors as negative control. c , d Strain APG-1 harboring plasmids pET-A and pCDF-EV1. e , f Strain APG-2 harboring plasmids pET-A and pCDF-EV2. g , h Strain APG-3 harboring plasmids pET-A and pCDF-EV3. a , c , e , g HPLC analysis of metabolites extracted from cell pellets. b , d , f , h HPLC analysis of metabolites extracted from the fermentation broth. i The titers of HIBP produced by the strains APG-1, APG-2 and APG-3. j The titers of PIVP produced by the strains APG-1, APG-2 and APG-3

    Journal: Microbial Cell Factories

    Article Title: Biosynthesis of phlorisovalerophenone and 4-hydroxy-6-isobutyl-2-pyrone in Escherichia coli from glucose

    doi: 10.1186/s12934-016-0549-9

    Figure Lengend Snippet: Engineered synthesis of 4-hydroxy-6-isovaleryl-2-pyrone (HIBP) and phlorisovalerophenone (PIVP) by E. coli strains. a , b Strain APG-0 harboring empty vectors as negative control. c , d Strain APG-1 harboring plasmids pET-A and pCDF-EV1. e , f Strain APG-2 harboring plasmids pET-A and pCDF-EV2. g , h Strain APG-3 harboring plasmids pET-A and pCDF-EV3. a , c , e , g HPLC analysis of metabolites extracted from cell pellets. b , d , f , h HPLC analysis of metabolites extracted from the fermentation broth. i The titers of HIBP produced by the strains APG-1, APG-2 and APG-3. j The titers of PIVP produced by the strains APG-1, APG-2 and APG-3

    Article Snippet: After crude extraction using the above protocol, purification of HIBP and PIVP was conducted by semi-preparative HPLC performed on a Shimadzu LC-6 AD with SPD-20A detector.

    Techniques: Negative Control, Positron Emission Tomography, High Performance Liquid Chromatography, Produced