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Activation of TCR-γδ cells by tumor cells is dependent upon the intracellular levels and activity of <t>HMGR.</t> (A) Mevastatin prevents response of the TCR-γδ T cell clone G2B9 stimulated by Daudi and YMB-1 ligands, but has no effect on stimulation with IPP or PHA. Results show TNFα mean release ± SD. (B) HMGR protein levels are down-regulated by farnesol or 7-DHC as detected by immunoprecipitation and Western blot. (C) Treatment of Daudi cells with farnesol or 7-DHC prevents stimulation of the G2B9 cells. In control experiments the same treatments of Daudi cells did not affect stimulation with exogenous IPP.
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Activation of TCR-γδ cells by tumor cells is dependent upon the intracellular levels and activity of <t>HMGR.</t> (A) Mevastatin prevents response of the TCR-γδ T cell clone G2B9 stimulated by Daudi and YMB-1 ligands, but has no effect on stimulation with IPP or PHA. Results show TNFα mean release ± SD. (B) HMGR protein levels are down-regulated by farnesol or 7-DHC as detected by immunoprecipitation and Western blot. (C) Treatment of Daudi cells with farnesol or 7-DHC prevents stimulation of the G2B9 cells. In control experiments the same treatments of Daudi cells did not affect stimulation with exogenous IPP.
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Activation of TCR-γδ cells by tumor cells is dependent upon the intracellular levels and activity of <t>HMGR.</t> (A) Mevastatin prevents response of the TCR-γδ T cell clone G2B9 stimulated by Daudi and YMB-1 ligands, but has no effect on stimulation with IPP or PHA. Results show TNFα mean release ± SD. (B) HMGR protein levels are down-regulated by farnesol or 7-DHC as detected by immunoprecipitation and Western blot. (C) Treatment of Daudi cells with farnesol or 7-DHC prevents stimulation of the G2B9 cells. In control experiments the same treatments of Daudi cells did not affect stimulation with exogenous IPP.
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Activation of TCR-γδ cells by tumor cells is dependent upon the intracellular levels and activity of <t>HMGR.</t> (A) Mevastatin prevents response of the TCR-γδ T cell clone G2B9 stimulated by Daudi and YMB-1 ligands, but has no effect on stimulation with IPP or PHA. Results show TNFα mean release ± SD. (B) HMGR protein levels are down-regulated by farnesol or 7-DHC as detected by immunoprecipitation and Western blot. (C) Treatment of Daudi cells with farnesol or 7-DHC prevents stimulation of the G2B9 cells. In control experiments the same treatments of Daudi cells did not affect stimulation with exogenous IPP.
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Activation of TCR-γδ cells by tumor cells is dependent upon the intracellular levels and activity of <t>HMGR.</t> (A) Mevastatin prevents response of the TCR-γδ T cell clone G2B9 stimulated by Daudi and YMB-1 ligands, but has no effect on stimulation with IPP or PHA. Results show TNFα mean release ± SD. (B) HMGR protein levels are down-regulated by farnesol or 7-DHC as detected by immunoprecipitation and Western blot. (C) Treatment of Daudi cells with farnesol or 7-DHC prevents stimulation of the G2B9 cells. In control experiments the same treatments of Daudi cells did not affect stimulation with exogenous IPP.
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Activation of TCR-γδ cells by tumor cells is dependent upon the intracellular levels and activity of <t>HMGR.</t> (A) Mevastatin prevents response of the TCR-γδ T cell clone G2B9 stimulated by Daudi and YMB-1 ligands, but has no effect on stimulation with IPP or PHA. Results show TNFα mean release ± SD. (B) HMGR protein levels are down-regulated by farnesol or 7-DHC as detected by immunoprecipitation and Western blot. (C) Treatment of Daudi cells with farnesol or 7-DHC prevents stimulation of the G2B9 cells. In control experiments the same treatments of Daudi cells did not affect stimulation with exogenous IPP.
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Activation of TCR-γδ cells by tumor cells is dependent upon the intracellular levels and activity of <t>HMGR.</t> (A) Mevastatin prevents response of the TCR-γδ T cell clone G2B9 stimulated by Daudi and YMB-1 ligands, but has no effect on stimulation with IPP or PHA. Results show TNFα mean release ± SD. (B) HMGR protein levels are down-regulated by farnesol or 7-DHC as detected by immunoprecipitation and Western blot. (C) Treatment of Daudi cells with farnesol or 7-DHC prevents stimulation of the G2B9 cells. In control experiments the same treatments of Daudi cells did not affect stimulation with exogenous IPP.
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Image Search Results


Activation of TCR-γδ cells by tumor cells is dependent upon the intracellular levels and activity of HMGR. (A) Mevastatin prevents response of the TCR-γδ T cell clone G2B9 stimulated by Daudi and YMB-1 ligands, but has no effect on stimulation with IPP or PHA. Results show TNFα mean release ± SD. (B) HMGR protein levels are down-regulated by farnesol or 7-DHC as detected by immunoprecipitation and Western blot. (C) Treatment of Daudi cells with farnesol or 7-DHC prevents stimulation of the G2B9 cells. In control experiments the same treatments of Daudi cells did not affect stimulation with exogenous IPP.

Journal: The Journal of Experimental Medicine

Article Title: Human T Cell Receptor γδ Cells Recognize Endogenous Mevalonate Metabolites in Tumor Cells

doi: 10.1084/jem.20021500

Figure Lengend Snippet: Activation of TCR-γδ cells by tumor cells is dependent upon the intracellular levels and activity of HMGR. (A) Mevastatin prevents response of the TCR-γδ T cell clone G2B9 stimulated by Daudi and YMB-1 ligands, but has no effect on stimulation with IPP or PHA. Results show TNFα mean release ± SD. (B) HMGR protein levels are down-regulated by farnesol or 7-DHC as detected by immunoprecipitation and Western blot. (C) Treatment of Daudi cells with farnesol or 7-DHC prevents stimulation of the G2B9 cells. In control experiments the same treatments of Daudi cells did not affect stimulation with exogenous IPP.

Article Snippet: Hamster HMGR cDNA (pRed-227; American Type Culture Collection) was subcloned into the XhoI/NotI sites of the BCMGSNeo expression vector.

Techniques: Activation Assay, Activity Assay, Immunoprecipitation, Western Blot, Control

HMGR-Daudi transfectants are potent stimulators. (A) Daudi cells transfected with the HMGR gene express higher levels of HMGR protein (bold line) than nontransfected Daudi cells (thin line). No difference between the two cell types was detected using an irrelevant mAb (dotted lines). (B) Daudi-HMGR cells (black bars) show increased capacity to stimulate Vγ9/Vδ2 cells as compared with nontransfected Daudi cells (white bars). Mevastatin was added at suboptimal doses (10 μM) 2, 6, or 12 h before incubation with T cells. Stimulation with IPP was used as positive control.

Journal: The Journal of Experimental Medicine

Article Title: Human T Cell Receptor γδ Cells Recognize Endogenous Mevalonate Metabolites in Tumor Cells

doi: 10.1084/jem.20021500

Figure Lengend Snippet: HMGR-Daudi transfectants are potent stimulators. (A) Daudi cells transfected with the HMGR gene express higher levels of HMGR protein (bold line) than nontransfected Daudi cells (thin line). No difference between the two cell types was detected using an irrelevant mAb (dotted lines). (B) Daudi-HMGR cells (black bars) show increased capacity to stimulate Vγ9/Vδ2 cells as compared with nontransfected Daudi cells (white bars). Mevastatin was added at suboptimal doses (10 μM) 2, 6, or 12 h before incubation with T cells. Stimulation with IPP was used as positive control.

Article Snippet: Hamster HMGR cDNA (pRed-227; American Type Culture Collection) was subcloned into the XhoI/NotI sites of the BCMGSNeo expression vector.

Techniques: Transfection, Incubation, Positive Control

Products on the Market Containing Thimerosal

Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: Seven Common Allergen Groups Causing Eyelid Dermatitis: Education and Avoidance Strategies

doi: 10.2147/OPTH.S297754

Figure Lengend Snippet: Products on the Market Containing Thimerosal

Article Snippet: Poly-Pred Ophthalmic Suspension (RX) 5 mL , Allergan.

Techniques: Ophthalmic Suspension, Suspension, Sterility