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Journal: Open Forum Infectious Diseases
Article Title: Laboratory Evaluation Links Some False-Positive COVID-19 Antigen Test Results Observed in a Field Study to a Specific Lot of Test Strips
doi: 10.1093/ofid/ofac701
Figure Lengend Snippet: Clinical false-positive rate of antigen rapid diagnostic tests (Ag-RDTs) among participants in a coronavirus disease 2019 (COVID-19) household-transmission field study and subsequent laboratory evaluation of technical false-positive rates among Ag-RDT strip lots. A , The biweekly clinical false-positive rate for nasal swab Ag-RDT, defined as a positive Ag-RDT at the same timepoint as negative results by reverse-transcription quantitative polymerase chain reaction (RT-qPCR) in saliva, nasal swabs, and oropharyngeal swab specimens. The proportions displayed below each month represent the number of clinical false-positive results over the total number of false-positive and true-negative Ag-RDT results in the field study during each period. Error bars represent 95% confidence interval (CI). B , Laboratory evaluation of the technical false-positive rate for 4 Ag-RDT strip lots was performed using severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–negative human nasal fluid (see Methods). The proportion of technical false-positive reads to all reads by readers blinded to experimental conditions is displayed below each lot number. P values were obtained using an upper-tailed Fisher exact test. Additional details are provided in .
Article Snippet: We created contrived specimens using heat-inactivated
Techniques: Diagnostic Assay, Transmission Assay, Stripping Membranes, Real-time Polymerase Chain Reaction, Quantitative RT-PCR