Journal: bioRxiv

Article Title: Orai1-mediated Ca 2+ Entry Regulates Lipolysis and Mitochondrial Activation in Brown Adipose Thermogenesis

doi: 10.64898/2026.04.24.718619

Figure Lengend Snippet: a Model illustrating that cold-induced Ca 2+ influx via Orai1 is essential for mitochondrial Ca 2+ uptake and subsequent thermogenesis. b Rhod-2 fluorescence imaging of mitochondrial Ca 2+ in siCtrl and siOrai1 cells. c Quantification of mitochondrial Ca 2+ uptake following temperature shifts (37 °C to 15 °C). d Transmission electron microscopy images of BAT from 6 hr cold-exposed control and Orai1 BKO mice. e Western blot analysis of OXPHOS proteins in control and Orai1 BKO BAT after cold exposure (4 °C) for 6 hrs. f Western blot analysis of OXPHOS proteins during differentiation of preadipocytes to brown adipocytes treated with siOrai1. g Mitochondrial DNA copy number measured in control and Orai1 -deficient cells with CL-316,243 treatment. h OCR analysis of Orai1 -deficient adipocytes treated with CL-316,243. i FAO assay of Orai1 -deficient adipocytes treated with CL-316,243. Data are presented as mean ± SEM. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Fully differentiated adipocytes (day 8) were used for in vitro experiments. hTERT-immortalized human brown preadipocytes (ATCC-CRL-4062) were maintained in DMEM/F12 medium supplemented with 10 % FBS.

Techniques: Fluorescence, Imaging, Transmission Assay, Electron Microscopy, Control, Western Blot

Journal: bioRxiv

Article Title: Scalable longitudinal imaging and transcriptomics of cells in dynamic enclosures

doi: 10.64898/2026.05.05.723030

Figure Lengend Snippet: A: UMAP based on transcriptomic data from primary human preadipocytes differentiated for seven days on a fibronectin-coated flow cell. The colors correspond to different clusters based on transcriptomic analysis. B: Transcriptomic UMAP colored by the lipid accumulation score, defined as the ratio between the BODIPY stain and the nuclear stain in each CCE. The insets show examples of cells that are very close in gene expression space but differ in their lipid content. C: violin plots depicting the distribution of lipid accumulation scores (y axis) across the transcriptomic clusters (x axis). D: actual (x axis) vs predicted (y axis) lipid accumulation scores from the elastic net model. The plot is for the held-out test set (20% of the total data). E: Euler diagram showing the overlap between top-20 differentially expressed genes between transcriptomic clusters (blue) and model-selected predictors of lipid accumulation (pink). F: average Log2 fold-change between clusters (x axis) vs absolute model coefficient (y axis) for the genes selected by the model. The red color indicates genes that are among the top-20 differentially expressed genes between transcriptomic clusters. G: Gene expression UMAP colored by the top-3 positive predictors identified by the model, showing that the expression values of these genes are uniformly distributed across the UMAP based on global transcriptomic differences. H: UMAP based on transcriptomic data for BV2 mouse microglial cells. The colors correspond to different clusters based on transcriptomic analysis. I: transcriptomic UMAP colored by phagocytic activity as measured by pHrodo™ intensity after four hours. J: UMAP based on DINOv2 features, colored by phagocytic activity showing a greater degree of separation between high vs low phagocytic scores, compared to the transcriptomic UMAP in panel H. K: violin plots depicting the distribution of phagocytic scores (y axis) across the transcriptomic clusters (x axis). L: R 2 performance of elastic net models trained on expression-only features, DINOv2-only features or a combination of the two (x axis). The data refers to the held-out test set (20% of the total data). M: actual (x axis) vs predicted (y axis) phagocytic scores from the elastic net model using the combined expression and DINOv2 features. The plot is for the held-out test set (20% of the total data). N: Euler plot showing the overlap between top-20 differentially expressed genes between transcriptomic clusters (blue) and model-selected predictors of phagocytic activity (pink). O: average Log2 fold-change between clusters (x axis) vs absolute models coefficient (y axis) for the genes selected by the expression-only model. The red color indicates genes that are among the top-20 differentially expressed genes between transcriptomic clusters. P: ridge plots displaying the expression level (x axis) of Gpnmb and Clec4e across transcriptomic clusters (x axis). These two genes are among the top positive predictors for the gene expression-based model and have clear mechanistic evidence linking them to the phagocytosis process. However, their expression is very similar across all the transcriptomic clusters.

Article Snippet: Adipogenesis was induced using Adipocytes Differentiation Toolkit for Adipose Derived MSCs and Preadipocytes (ATCC, # PCS-500-050).

Techniques: Staining, Gene Expression, Expressing, Activity Assay

Journal: iScience

Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation

doi: 10.1016/j.isci.2024.109672

Figure Lengend Snippet:

Article Snippet: Subcutaneous Preadipocyte Differentiation Medium , ZenBio , Cat#DM-2.

Techniques: Infection, Recombinant, Electron Microscopy, SYBR Green Assay, Lysis, Protease Inhibitor, Plasmid Preparation, XF Assay, Isolation, Quantitation Assay, Bicinchoninic Acid Protein Assay, Software