Journal: Antioxidants

Article Title: Tetraselmis chuii Supplementation Increases Skeletal Muscle Nuclear Factor Erythroid 2-Related Factor 2 and Antioxidant Enzyme Gene Expression, and Peak Oxygen Uptake in Healthy Adults: A Randomised Crossover Trial

doi: 10.3390/antiox14040435

Figure Lengend Snippet: Gene names, gene symbols and ThermoFisher Scientific Assay IDs for human skeletal muscle OpenArray™ gene expression analyses.

Article Snippet: Peroxiredoxin 2 , PRDX2 , Hs00853603_s1.

Techniques: Gene Expression, Binding Assay

Journal: Endocrinology

Article Title: Periovulatory expression of hydrogen peroxide-induced sulfiredoxin and peroxiredoxin 2 in the rat ovary: gonadotropin regulation and potential modification.

doi: 10.1210/en.2012-1414

Figure Lengend Snippet: FIG. 6. Regulation of ovarian PRDX2 expression by gonadotropins. A, Aliquots of total RNA (20 g) isolated from ovaries at the indicated time intervals after eCG/hCG stimulation were assayed for PRDX2 mRNA levels by Northern blotting using a rat PRDX2 cDNA probe. The expression of 28S ribosomal RNA was used as an internal standard. The PRDX2 transcript was quantified using a phosphorimager and normalized for 28S RNA levels from three independently performed experiments (mean SEM). B, In situ localization of ovarian PRDX2 mRNA. Ovarian sections were hybridized with a 35S-labeled PRDX2 cRNA probe. Photomicrographs were taken under bright-field (a, c, and e) and corresponding dark-field (b, d, and f) illumination. Arrowhead, Oocyte; arrow, theca cell; asterisk, growing follicle; P, preovulatory follicle; CL, corpus luteum, 20. C, Western blotting analysis of PRDX2 protein in the ovary. Ovarian lysates were separated on a 12% SDS-PAGE gel in the presence (reducing) or absence (nonreducing) of 10% 2-mercaptoethanol. The quantified PRDX2 protein levels are expressed as a mean SEM of two or three independently performed experiments.

Article Snippet: Blots were incubated with primary antibodies against Srx, PRDX2, -PRDX-SO2/3, p-Akt (Cell Signaling Technology, Beverly, MA), Akt (Cell Signaling Technology), pPTEN (Cell Signaling Technology), or PTEN (Cell Signaling Technology).

Techniques: Expressing, Isolation, Northern Blot, In Situ, Labeling, Western Blot, SDS Page

Journal: Endocrinology

Article Title: Periovulatory expression of hydrogen peroxide-induced sulfiredoxin and peroxiredoxin 2 in the rat ovary: gonadotropin regulation and potential modification.

doi: 10.1210/en.2012-1414

Figure Lengend Snippet: FIG. 7. Modification of PRDX2 in preovulatory follicles after gonadotropin treatment. Preovulatory follicles were dissected from ovaries obtained at different times after eCG/hCG treatment. Cell lysates of preovulatory follicles were analyzed by SDS-PAGE (A) or two- dimensional PAGE (B) followed by immunoblot analysis with antibodies specific to Srx, sulfinic/sulfonic 2-Cys PRDXs (PRDX-SO2/3), PRDX2, and GAPDH. Lysates of HeLa cells exposed to 1 mM H2O2 for 10 min were used as a positive control for the expression of hyperoxidized 2-Cys PRDXs. The region on the two-dimensional immunoblots corresponds to a molecular mass of 22–28 kDa (vertical) and isoelectric points of 5.2–5.6 (horizontal). The band intensities of each sample were measured and the ratio of hyperoxidized (Ox) to reduced (Re) PRDX2 was used to plot the graph (B, lower panel). Each point on the graph represents mean SEM of three independently performed experiments. *, P 0.05 vs. 0 h.

Article Snippet: Blots were incubated with primary antibodies against Srx, PRDX2, -PRDX-SO2/3, p-Akt (Cell Signaling Technology, Beverly, MA), Akt (Cell Signaling Technology), pPTEN (Cell Signaling Technology), or PTEN (Cell Signaling Technology).

Techniques: Modification, SDS Page, Western Blot, Positive Control, Expressing

Journal: Molecular medicine reports

Article Title: Proteomics changes after negative pressure wound therapy in diabetic foot ulcers.

doi: 10.3892/mmr.2021.12474

Figure Lengend Snippet: Figure 4. Validation of the protein expression levels of CTSS, PROS1, ITIH4 and PRDX2. Wound granulation tissues from eight patients with diabetic foot ulcers before and after NPWT were collected and subjected to western blotting. Primary antibodies against (A) CTSS, (B) PROS1, (C) ITIH4 and (D) PRDX2 were used to detect differences in protein levels. (A1‑D1) Protein bands from four representative patients prior to NPWT (designated as minus symbol ‘‑’) and following NPWT (designated as plus symbol ‘+’) are presented in the left panel. Protein samples from the same patients were loaded next to each other. (A2‑D2) Then, eight pairs of protein samples from eight patients were analyzed by ImageJ and are depicted as paired dots in the right panel. (A3‑D3) The gray level of the target protein was compared with the internal reference (β‑actin) gray level to obtain the relative expression value. Paired t‑tests were performed and significant changes are indicated by ***P<0.001 vs. before NPWT. CTSS, cathepsin; PROS1, protein S isoform 1; ITIH4, inter α‑trypsin inhibitor heavy chain H4; PRDX2, peroxiredoxin‑2; NPWT, negative‑pressure wound therapy.

Article Snippet: The levels of CTSS, PrdX2, ProS1 and iTiH4 in the peripheral blood of the subjects were determined using the following eliSa kits (Cusabio Biotech Co., Ltd.) according to the protocols provided by the manufacturer: CTSS (cat. no. CSB‐E13722h), PRDX2 (cat. no. CSB‐EL018654HU), PROS1 (cat. no. CSB‐E09903h) and ITIH4 (cat. no. CSB‐E17022h).

Techniques: Biomarker Discovery, Expressing, Western Blot

Journal: Molecular medicine reports

Article Title: Proteomics changes after negative pressure wound therapy in diabetic foot ulcers.

doi: 10.3892/mmr.2021.12474

Figure Lengend Snippet: Figure 5. ELISA analysis of serum CTSS, ITIH4, PROS1 and PRDX2 levels in patients before and after NPWT. Blood samples from 17 patients were collected and serum CTSS, ITIH4, PROS1 and PRDX2 levels were measured with ELISA. Serum (A1) CTSS, (B1) ITIH4, (C1) PROS1 and (D1) PRDX2 prior to and following NPWT are depicted as paired dots. The changes in serum (A2) CTSS, (B2) ITIH4, (C2) PROS1 and (D2) PRDX2 levels prior to and following NPWT are shown by histograms. Paired t‑tests were performed and significant changes are indicated by *P<0.05, **P<0.01, ***P<0.001 vs. before NPWT. CTSS, cathepsin; PROS1, protein S isoform 1; ITIH4, inter α‑trypsin inhibitor heavy chain H4; PRDX2, peroxiredoxin‑2; NPWT, negative‑pressure wound therapy.

Article Snippet: The levels of CTSS, PrdX2, ProS1 and iTiH4 in the peripheral blood of the subjects were determined using the following eliSa kits (Cusabio Biotech Co., Ltd.) according to the protocols provided by the manufacturer: CTSS (cat. no. CSB‐E13722h), PRDX2 (cat. no. CSB‐EL018654HU), PROS1 (cat. no. CSB‐E09903h) and ITIH4 (cat. no. CSB‐E17022h).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Cells

Article Title: Identification of Targets from LRRK2 Rescue Phenotypes

doi: 10.3390/cells10010076

Figure Lengend Snippet: List of functional clusters that were enriched using DAVID.

Article Snippet: PRDX2 plasmid (RC207413) was purchased from Origene.

Techniques: Functional Assay

Journal: Cells

Article Title: Identification of Targets from LRRK2 Rescue Phenotypes

doi: 10.3390/cells10010076

Figure Lengend Snippet: Validation of PRDX2. ( a ) Functional annotation enrichment on downregulated differential transcripts in pathogenic variant reveals significant enrichment of PRDX2 -centred oxidoreductase pathway genes by the DAVID enrichment tool (nominal p -value <0.01). ( b ) MetaCore gene networks on the core nodes of oxidoreductase annotation clusters demonstrate PRDX2 acts as a key player in PTEN , CREB1, and FLRE pathways. ( c ) Histogram plot shows differential FPKM levels across biological fly phenotypes for two PRDX2 isoforms (TCONS_00023592 and TCONS_00023593; FDR p < 0.05). ( d ) Normalized Jafrac1 (Drosophila PRDX2 orthologue) mRNA levels measured by quantitative PCR in RNA isolated from 60-day-old fly heads. Data show mean and s.d. of three independent biological replicates. Significance on the graph: * p < 0.05.

Article Snippet: PRDX2 plasmid (RC207413) was purchased from Origene.

Techniques: Biomarker Discovery, Functional Assay, Variant Assay, Real-time Polymerase Chain Reaction, Isolation