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Image Search Results
Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology
Article Title: Syringetin relieves bone cancer pain in rats induced by breast cancer cells through the ESR1/PRDM2 axis.
doi: 10.4196/kjpp.24.303
Figure Lengend Snippet: Fig. 3. Syringetin inhibited the malignant characteristics of rat breast cancer cells SHZ-88 and the expression of ESR1 protein. (A) Cell viability was detected by CCK8 after SHZ-88 cells were intervened with 2.5 μM, 5 μM, and 10 μM Syringetin. (B) TUNEL staining was employed to detect the cell apoptosis under the intervention of 10 μM Syringetin (200×). (C) Transwell was applied to detect cell migration under the intervention of 10 μM Syringetin. (D) WB detection of the expression of ESR1 and PRDM2 proteins in cells under the intervention of 10 μM Syringetin. N = 3 replicates/group. ESR1, estrogen receptor 1; CCK8, Cell-Counting-Kit-8; TUNEL, Terminal transferase dUTP Nick End Labeling; WB, Western blotting; DAPI, 4,6-diamidine- 2-phenylindole dihydrochloride; PRDM2, positive regulatory domain zinc finger protein2. *p < 0.05 vs. Control.
Article Snippet: Then, first antibodies were added onto the membrane, including ESR1 (21244-1-AP, 1:2,000, Proteintech),
Techniques: Expressing, TUNEL Assay, Staining, Migration, Cell Counting, End Labeling, Western Blot, Control
Journal: The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology
Article Title: Syringetin relieves bone cancer pain in rats induced by breast cancer cells through the ESR1/PRDM2 axis.
doi: 10.4196/kjpp.24.303
Figure Lengend Snippet: Fig. 4. Syringetin inhibited ESR1 expression and alleviated bone cancer pain (BCP) induced by breast cancer cells in rats. 4 × 10 4 SHZ-88 cells were implanted into the bone marrow cavity of the hind tibia of rats, which was administered orally with 15 mg/kg/day Syringetin for 21 days. (A) Histopathological status of rat tibia tissue stained with HE (100× and 400×). (B) AS scores and PWT scores. (C) WB detection of ESR1 and PRDM2 ex- pression in tibial bone marrow tissue. N = 9 replicates/group. ESR1, estrogen receptor 1; HE, hematoxylin-eosin; AS, Ambulatory score; PWT, paw with- drawal threshold; WB, Western blotting; PRDM2, positive regulatory domain zinc finger protein2. *p < 0.05 vs. Sham, #p < 0.05 vs. BCP.
Article Snippet: Then, first antibodies were added onto the membrane, including ESR1 (21244-1-AP, 1:2,000, Proteintech),
Techniques: Expressing, Staining, Western Blot