pr-1432 Search Results


recombinant hbsag adw subviral particles  (Jena Bioscience)
93
Jena Bioscience recombinant hbsag adw subviral particles
Recombinant Hbsag Adw Subviral Particles, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant hbsag adw subviral particles/product/Jena Bioscience
Average 93 stars, based on 1 article reviews
recombinant hbsag adw subviral particles - by Bioz Stars, 2026-02
93/100 stars
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page purified primer illumina-sgrna_seq  (Illumina Inc)
90
Illumina Inc page purified primer illumina-sgrna_seq
Page Purified Primer Illumina Sgrna Seq, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/page purified primer illumina-sgrna_seq/product/Illumina Inc
Average 90 stars, based on 1 article reviews
page purified primer illumina-sgrna_seq - by Bioz Stars, 2026-02
90/100 stars
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gp41  (Jena Bioscience)
92
Jena Bioscience gp41
Whole cell dot bot and Western blot assay screening of surface expression of the MPER. ( A ) Dots representing whole cells immunostained with 2F5 primary antibodies of viable (V) or heat-killed (HK) EcN-MPER and EcN from passages 1 (P1) and 30 (P30) of the overnight cultures. The recombinant HIV-1 <t>gp41</t> protein at concentrations of 15 and 150 ng was used as a positive control (PC). Detection was performed using the goat anti-human IgG IRDye® 800CW secondary antibody. ( B ) Western blot analysis of the outer membrane vesicles (OMVs) – rich fraction extracted from EcN-MPER culture supernatant. MPER was detected in the OMVs-rich fraction and positive control (EcN-MPER pellet) using HIV-1 gp41 (2F5) monoclonal antibody and rabbit anti-human HRP-conjugated IgG (Fc specific) secondary antibody. ( C ) DLS analysis of the OMVs fraction, and ( D ) negative stain TEM analysis of the same fraction
Gp41, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gp41/product/Jena Bioscience
Average 92 stars, based on 1 article reviews
gp41 - by Bioz Stars, 2026-02
92/100 stars
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gfp  (Promega)
90
Promega gfp
Whole cell dot bot and Western blot assay screening of surface expression of the MPER. ( A ) Dots representing whole cells immunostained with 2F5 primary antibodies of viable (V) or heat-killed (HK) EcN-MPER and EcN from passages 1 (P1) and 30 (P30) of the overnight cultures. The recombinant HIV-1 <t>gp41</t> protein at concentrations of 15 and 150 ng was used as a positive control (PC). Detection was performed using the goat anti-human IgG IRDye® 800CW secondary antibody. ( B ) Western blot analysis of the outer membrane vesicles (OMVs) – rich fraction extracted from EcN-MPER culture supernatant. MPER was detected in the OMVs-rich fraction and positive control (EcN-MPER pellet) using HIV-1 gp41 (2F5) monoclonal antibody and rabbit anti-human HRP-conjugated IgG (Fc specific) secondary antibody. ( C ) DLS analysis of the OMVs fraction, and ( D ) negative stain TEM analysis of the same fraction
Gfp, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfp/product/Promega
Average 90 stars, based on 1 article reviews
gfp - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
oa1 gpr143 polyclonal antibody, alexa fluor 647 conjugated  (Bioss)
N/A
G protein-coupled receptors (GPRs or GPCRs), are members of the largest protein family and play a role in many different stimulus-response pathways. G-protein coupled receptors mediate extracellular signals into intracellular signals (G-protein activation). They respond
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rabbit anti human gpr143 polyclonal antibody  (Cusabio)
N/A
Rabbit anti Human GPR143 Polyclonal Antibody
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gpr142 polyclonal antibody  (Elabscience)
N/A
GPR142 is a member of the rhodopsin family of G protein coupled receptors GPRs
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gpr142 rabbit polyclonal antibody  (OriGene)
N/A
GPR142 rabbit polyclonal antibody
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gpr142 antibody  (Proteintech)
N/A
18820 1 AP targets GPR142 in WB FC ELISA applications and shows reactivity with human samples
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gpr142 nm 181749 mouse tagged orf clone lentiviral particle  (OriGene)
N/A
Lenti ORF particles Gpr142 GFP tagged Mouse G protein coupled receptor 142 Gpr142 200ul 10 7 TU mL
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human oa1/gpr143 (np_000264) versaclone cdna  (R&D Systems)
N/A
Human OA1/GPR143 (NP_000264) VersaClone cDNA
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oa1 gpr143 human gene knockout kit  (OriGene)
N/A
GPR143 KN2 0 Human gene knockout kit via CRISPR non homology mediated
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Image Search Results


Whole cell dot bot and Western blot assay screening of surface expression of the MPER. ( A ) Dots representing whole cells immunostained with 2F5 primary antibodies of viable (V) or heat-killed (HK) EcN-MPER and EcN from passages 1 (P1) and 30 (P30) of the overnight cultures. The recombinant HIV-1 gp41 protein at concentrations of 15 and 150 ng was used as a positive control (PC). Detection was performed using the goat anti-human IgG IRDye® 800CW secondary antibody. ( B ) Western blot analysis of the outer membrane vesicles (OMVs) – rich fraction extracted from EcN-MPER culture supernatant. MPER was detected in the OMVs-rich fraction and positive control (EcN-MPER pellet) using HIV-1 gp41 (2F5) monoclonal antibody and rabbit anti-human HRP-conjugated IgG (Fc specific) secondary antibody. ( C ) DLS analysis of the OMVs fraction, and ( D ) negative stain TEM analysis of the same fraction

Journal: Microbial Cell Factories

Article Title: Stable expression of HIV-1 MPER extended epitope on the surface of the recombinant probiotic bacteria Escherichia Coli Nissle 1917 using CRISPR/Cas9

doi: 10.1186/s12934-023-02290-0

Figure Lengend Snippet: Whole cell dot bot and Western blot assay screening of surface expression of the MPER. ( A ) Dots representing whole cells immunostained with 2F5 primary antibodies of viable (V) or heat-killed (HK) EcN-MPER and EcN from passages 1 (P1) and 30 (P30) of the overnight cultures. The recombinant HIV-1 gp41 protein at concentrations of 15 and 150 ng was used as a positive control (PC). Detection was performed using the goat anti-human IgG IRDye® 800CW secondary antibody. ( B ) Western blot analysis of the outer membrane vesicles (OMVs) – rich fraction extracted from EcN-MPER culture supernatant. MPER was detected in the OMVs-rich fraction and positive control (EcN-MPER pellet) using HIV-1 gp41 (2F5) monoclonal antibody and rabbit anti-human HRP-conjugated IgG (Fc specific) secondary antibody. ( C ) DLS analysis of the OMVs fraction, and ( D ) negative stain TEM analysis of the same fraction

Article Snippet: A standard curve was prepared with the recombinant HIV-1 M gp41 (Jena Bioscience, Jena, Germany) using 0.5, 1, 2, 5, 10 and 15 μg/ml dilutions of the protein.

Techniques: Western Blot, Expressing, Recombinant, Positive Control, Membrane, Staining

Quantification of MPER concentration in bacteria via indirect ELISA. ( A ) From recombinant (EcN-MPER) and non-modified (EcN) bacterial lysates. The antigen concentration was determined using a standard curve plotted with absorbance readings applying a serially diluted HIV-1 gp41 antigen. ( B ) Quantitation of surface-expressed MPER through a whole cell ELISA of non-modified EcN and recombinant EcN-MPER from combined passages 1, 15, 20, 25 and 30. Samples with viable bacteria are marked with V and with heat-killed – HK. Bars are shown with mean and standard deviation of the 5 different passages. Comparative analysis was performed using ( A ) Welch’s t test or ( B ) Mann Whitney test; where * p < 0.05 and ** p < 0.005

Journal: Microbial Cell Factories

Article Title: Stable expression of HIV-1 MPER extended epitope on the surface of the recombinant probiotic bacteria Escherichia Coli Nissle 1917 using CRISPR/Cas9

doi: 10.1186/s12934-023-02290-0

Figure Lengend Snippet: Quantification of MPER concentration in bacteria via indirect ELISA. ( A ) From recombinant (EcN-MPER) and non-modified (EcN) bacterial lysates. The antigen concentration was determined using a standard curve plotted with absorbance readings applying a serially diluted HIV-1 gp41 antigen. ( B ) Quantitation of surface-expressed MPER through a whole cell ELISA of non-modified EcN and recombinant EcN-MPER from combined passages 1, 15, 20, 25 and 30. Samples with viable bacteria are marked with V and with heat-killed – HK. Bars are shown with mean and standard deviation of the 5 different passages. Comparative analysis was performed using ( A ) Welch’s t test or ( B ) Mann Whitney test; where * p < 0.05 and ** p < 0.005

Article Snippet: A standard curve was prepared with the recombinant HIV-1 M gp41 (Jena Bioscience, Jena, Germany) using 0.5, 1, 2, 5, 10 and 15 μg/ml dilutions of the protein.

Techniques: Concentration Assay, Bacteria, Indirect ELISA, Recombinant, Modification, Quantitation Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation, MANN-WHITNEY