Journal: Molecular Pharmacology

Article Title: Discovery of RGS2-FBXO44 interaction inhibitors using a cell-based NanoBit assay

doi: 10.1016/j.molpha.2025.100030

Figure Lengend Snippet: High-throughput screen to identify RGS2-FBXO44 PPI inhibitors. (A) Representation of the Z-factor obtained in the primary screen, as determined by comparing the single clone (#6) RGS2-SmBiT/LgBit-FBXO44 cell line to nontransfected HEK-293T cells. Luminescence was normalized to viability (RLU/RFU) and the average signal in #6 was set to 100%. Z’ was calculated to be 0.56. N = 70 for each condition. (B) Screening funnel with hit rates for primary screen and hit confirmation. (C) Scatter plot for hit confirmation. Data presented as normalized luminescence (RLU/RFU) and expressed as percentage of nontreated cells. Blue line represents average response in control cells; Red dotted line represents 50% inhibition of NanoBit signal; Confirmed hits are highlighted in black. RLU, relative luminescence units.

Article Snippet: To identify inhibitors of the RGS2-FBXO44 interaction, we screened 1600 compounds from the Life Chemicals PPI Fragment library.

Techniques: High Throughput Screening Assay, Control, Inhibition

Journal: Genome Biology

Article Title: Genome-wide prioritization of disease genes and identification of disease-disease associations from an integrated human functional linkage network

doi: 10.1186/gb-2009-10-9-r91

Figure Lengend Snippet: Data sources for FLN construction

Article Snippet: Masspec , PPI from large-scale mass spectrometry experiments [ ] , 2,046 , 1,159.

Techniques: Mass Spectrometry, Expressing, Functional Assay