Journal: Redox biology

Article Title: The E3 ubiquitin ligase TRIM31 is involved in cerebral ischemic injury by promoting degradation of TIGAR.

doi: 10.1016/j.redox.2021.102058

Figure Lengend Snippet: Fig. 4. TRIM31 deficiency maintained mitochondria homeostasis in brain of mice subjected to middle cerebral artery occlusion (MCAO). (A) TRIM31 deficiency improved ultrastructural defects of neuronal mitochondria from mice subjected to MCAO. (B–D) The effect of TRIM31 on the expression of protein related to mitochondrial dynamics, PGC-1α, MFN1, MEN2 and DRP1, in the mice brain after 24 h of MCAO. Scale bars: 10 μm. Data were presented as the mean ± SEM of 3 independent experiments. *p < 0.05 vs. indicated group.

Article Snippet: Primary antibodies against TRIM31 (12543-1-AP), G6PD (66 373-1-1 g), DRP1 (12957-1-AP), MFN1 (13798-1-AP), MFN2 (12186-1-AP) and PGC-1α (66 369-1-1 g) were from Proteintech (Wuhan, China).

Techniques: Expressing

Journal: Journal of Neuroinflammation

Article Title: Resolvin RvD2 reduces hypothalamic inflammation and rescues mice from diet-induced obesity

doi: 10.1186/s12974-016-0777-2

Figure Lengend Snippet: The impact of exogenous RvD2-injected icv on inflammatory and metabolic parameters in mice. Six-week-old Swiss mice were included in the study and fed a HF diet for 8 weeks before intracerebroventricular (icv) cannulation; after 1 week, mice were randomly selected for either saline (2 μl) or different amounts of RvD2 (2, 3 or 50 ng) icv treatment for 11 days ( a ). At the end of the experimental period, hypothalamic RNA was extracted and employed in real-time PCR determinations of GPR18 ( b ). Cumulative food intake ( c ) and body mass variation ( d ) were determined during the experimental period. At the end of the experimental period, the epididymal fat pad was measured ( e ). In addition, mice were submitted a glucose tolerance test, and results are expressed as the area under the curve (AUC) ( f ). Interleukin-6 (IL6) ( g ) and interleukin-10 (IL10) ( h ) transcripts were determined in samples from the hypothalamus, whereas uncoupling protein 1 (UCP1) ( i ) and PGC1a ( j ) transcripts were determined in samples from the brown adipose tissue. Some mice were subjected to indirect calorimetry, resulting in the values for O 2 consumption ( k ), CO 2 production ( l ) and respiratory quotient ( m ). In addition, some mice were subjected to a leptin tolerance test (LTT) and results are expressed as cumulative food intake during 12 h ( n ). In the experiments reported in panels a – j , n = 8; in the experiments reported in panels k – n , n = 5. * p < 0.05 vs. saline. Experiments for evaluation of 3 and 50 ng RvD2 in the hypothalamus were performed in different occasions. The differences obtained in some of the saline groups reflect interexperimental variability. For statistical analysis, group-specific saline control was considered as baseline

Article Snippet: TaqMan primers for PLA2 (Mm00448161_m1), 15-LOX (Mm00507789_m1), 5-LOX (Mm01182747_m1), GPR18 (Mm01224541_s1), TNFα (Mm00443258_m1), IL1β (Mm00434228_m1), IL6 (Mm00446190_m1), IL10 (Mm01288386_m1), PGC1α (Mm00447183_m1), UCP1 (Mm01244861_m1) and glyceraldehyde-3-phosphate dehydrogenase (GAPD) (#4352339E) were obtained from Applied Biosystems.

Techniques: Injection, Saline, Real-time Polymerase Chain Reaction, Control

Journal: PLoS ONE

Article Title: Aromatase deficiency in hematopoietic cells improves glucose tolerance in male mice through skeletal muscle-specific effects

doi: 10.1371/journal.pone.0227830

Figure Lengend Snippet: Primer sets employed.

Article Snippet: Cebpa , Mm00514283_s1 , Ppargc1a , Mm00447184_g1.

Techniques:

Journal: Pathology international

Article Title: FHL1: A novel diagnostic marker for papillary thyroid carcinoma.

doi: 10.1111/pin.13467

Figure Lengend Snippet: FIGURE 5 The expression of FHL1, BCL2, TLE1, KIT, PPARGC1A and GHR in papillary thyroid carcinoma (PTC) and the control group. FHL1 showed strong positivity in the nuclei and to a lesser extent in the cytoplasm of normal thyroid tissues adjacent to the tumour (the control group), but is not present in the PTC tissues. (p < 0.01). There was no significant difference in the expression of BCL2 between PTC and normal thyroid tissues (p > 0.05). The results showed that TLE1, KIT, PPARGC1A, and GHR were absent in both tumor and normal tissues. (magnification ×200). FHL1, four and a half LIM domains 1.

Article Snippet: Slides were immunostained with monoclonal antibodies against the BCL2 antigen (clone SP66, Maixin), CD117/C‐kit antigen (clone YR145, Maixin), and with monoclonal antibodies against the FHL1 (10991‐1‐AP, Proteintech Group, Inc.), PPARGC1A (Boster, China), and TLE1 antigen (Maixin).

Techniques: Expressing, Control