potato Search Results


potato virus yo pv 575  (ATCC)
93
ATCC potato virus yo pv 575
Potato Virus Yo Pv 575, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solanum tuberosum lectin stl  (Vector Laboratories)
92
Vector Laboratories solanum tuberosum lectin stl
Solanum Tuberosum Lectin Stl, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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potato dextrose agar pda  (Merck & Co)
86
Merck & Co potato dextrose agar pda
Potato Dextrose Agar Pda, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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potato dextrose agar  (Carolina Biological)
93
Carolina Biological potato dextrose agar
Potato Dextrose Agar, supplied by Carolina Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solanum tuberosum  (Vector Laboratories)
93
Vector Laboratories solanum tuberosum
All the lectins employed labelled the vomeronasal glands (VG) and the muco-microvillar complex, i.e. the superficial border of both vomeronasal epithelia. A, E . Labelling of the VNO at levels L3 and L8, respectively with the lectin SBA ( Glycine max ). In both cases the VG are strongly labelled. B. VVA ( Vicia villosa ) labelling at a central level L6 of the VNO shows a faint labelling of the VG. C, D. LEA ( Lycopersicon esculentum ) labels the vomeronasal epithelia, the NVNs, and the VG. LEA does not stain the caudal nasal nerve (asterisk in C). F, I, K, N. STA ( Solanum <t>tuberosum</t> ) lectin labelling at central levels (L4, L5) of the VNO stains the vomeronasal epithelia, vomeronasal nerves (NVN) and VG. G, H, J, L, M. UEA ( Ulex europaeus ) lectin stains vomeronasal epithelia, VG, but hardly stains NVNs. Vv, veins; directionality is indicated by the following: d, dorsal; l, lateral; m, medial; v, ventral. Scale bars: A-C, E, I, J = 500 μm; D, F, G = 250 μm; H = 100 μm; K-N = 50 μm.
Solanum Tuberosum, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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моделирование биотического стресса  (DSMZ)
90
DSMZ моделирование биотического стресса
Fig. 1. Callus Tissue with the formation of globular structures
моделирование биотического стресса, supplied by DSMZ, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glycerol  (Chem Impex International)
95
Chem Impex International glycerol
Fig. 1. Callus Tissue with the formation of globular structures
Glycerol, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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potato dextrose agar  (Valiant Co Ltd)
96
Valiant Co Ltd potato dextrose agar
Fig. 1. Callus Tissue with the formation of globular structures
Potato Dextrose Agar, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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potato dextrose broth  (Teknova)
93
Teknova potato dextrose broth
Fig. 1. Callus Tissue with the formation of globular structures
Potato Dextrose Broth, supplied by Teknova, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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potato virus v  (DSMZ)
93
DSMZ potato virus v
Fig. 1. Callus Tissue with the formation of globular structures
Potato Virus V, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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potato leafroll virus  (DSMZ)
93
DSMZ potato leafroll virus
Figure 1. (A) Sequence alignments of the ATPase domain of <t>PLRV_MP.</t> Multiple sequence alignments, generated using ClustalW, were manually corrected for domain superimpositions. The number(s) in brackets represent the number of amino acids. (B) Organization of functional motifs on the polypeptide chain of MP of PLRV. Schematics are drawn approximately to the scale and represent the approximate consensus of representative homologs. (C) I-TASSER predicted atomic model of PLRV_MP with all the putative ATP catalysis motifs. [Accession Numbers: Potato <t>leafroll</t> virus isolate PLRV-JF (PLRV JF): ADE93562.1, Potato leafroll virus isolate Anoquia (PLRV Antioquia): QES86451.1, Potato leafroll virus isolate Sta Rosa de Osos-santana (PLRV Santana): AEB78381.1, Potato leafroll virus isolate Turmeque-siguineque (PLRV Tur-sig): AEB78353.1, Potato leafroll virus isolate Sonson-tasajo (PLRV Sonson): AEB78367.1, Potato leafroll virus isolate Siachoque-jurubita (PLRV Jurubita): AEB78379.1, Potato leafroll virus isolate Pasto-el campanero2 (PLRV Pasto-el): AEB78377.1, Potato leafroll virus isolate Pasto-el campanero (PLRV Campa): AEB78375.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentina): ADE93558.1, Potato leafroll virus isolate Zipaquira-san Jorge (PLRV Zipaquir): AEB78361.1, Potato leafroll virus isolate Turmeque-siguineque2 (PLRV Turmeque): AEB78373.1, Potato leafroll virus isolate Ipiales-suras (PLRV Suras): AEB78385.1, France Potato leafroll virus strain CIP01 (PLRV CIP01): AAL77949.1, Potato leafroll virus isolate PLRV184 (PLRV 184): AYA73306.1, Potato leafroll virus isolate Villapinzon-bosabita (PLRV Villapinzo): AEB78359.1, Potato leafroll virus isolate Madrid-los arboles2 (PLRV Arboles): AEB78369.1, Potato leafroll virus isolate TZ:LR3M:11 (PLRV TZ:LR3M): AGN48055.1, Potato leafroll virus isolate PLRV171 (PLRV Canada): AYA73300.1, Colombia Potato leafroll virus isolate PLRV165 (PLRV Colombia): AYA73294.1, Tamil nadu Potato leafroll virus isolate OTNI-2 (PLRV Tamilnadu): AFJ11889.1, Jalandgar Potato leafroll virus isolate (PLRV Jalandgar): AFJ11863.1, Karlovce Potato leafroll virus isolate VIRUBRA 1/045 (PLRV VIRUBRA45): ACD93696.1, Australia Potato leafroll virus (PLRV Australia): QBO24572.1, Shimla Potato leafroll virus isolate PBI-6 (PLRV Shimla): AFJ11881.1, Potato leafroll virus (PLRV): QBO24572.1, Prague Potato leafroll virus isolate VIRUBRA 1/046 (PLRV VIRUBRA46): ACD93705.1, Potato leafroll virus isolate Say88 (PLRV Say88): QBO24572.1, Potato leafroll virus isolate Turmeque-siguineque3 (PLRV Siguineque3): AEB78383.1, Potato leafroll virus isolate Facatativa (PLRV Facatat): AEB78357.1, Germany Potato leafroll virus isolate PLRV-DSMZ (PLRV Germany): ADE93559.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentinian): ADE93558.1].
Potato Leafroll Virus, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chromobacterium pv104  (ATCC)
91
ATCC chromobacterium pv104
Figure 1. (A) Sequence alignments of the ATPase domain of <t>PLRV_MP.</t> Multiple sequence alignments, generated using ClustalW, were manually corrected for domain superimpositions. The number(s) in brackets represent the number of amino acids. (B) Organization of functional motifs on the polypeptide chain of MP of PLRV. Schematics are drawn approximately to the scale and represent the approximate consensus of representative homologs. (C) I-TASSER predicted atomic model of PLRV_MP with all the putative ATP catalysis motifs. [Accession Numbers: Potato <t>leafroll</t> virus isolate PLRV-JF (PLRV JF): ADE93562.1, Potato leafroll virus isolate Anoquia (PLRV Antioquia): QES86451.1, Potato leafroll virus isolate Sta Rosa de Osos-santana (PLRV Santana): AEB78381.1, Potato leafroll virus isolate Turmeque-siguineque (PLRV Tur-sig): AEB78353.1, Potato leafroll virus isolate Sonson-tasajo (PLRV Sonson): AEB78367.1, Potato leafroll virus isolate Siachoque-jurubita (PLRV Jurubita): AEB78379.1, Potato leafroll virus isolate Pasto-el campanero2 (PLRV Pasto-el): AEB78377.1, Potato leafroll virus isolate Pasto-el campanero (PLRV Campa): AEB78375.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentina): ADE93558.1, Potato leafroll virus isolate Zipaquira-san Jorge (PLRV Zipaquir): AEB78361.1, Potato leafroll virus isolate Turmeque-siguineque2 (PLRV Turmeque): AEB78373.1, Potato leafroll virus isolate Ipiales-suras (PLRV Suras): AEB78385.1, France Potato leafroll virus strain CIP01 (PLRV CIP01): AAL77949.1, Potato leafroll virus isolate PLRV184 (PLRV 184): AYA73306.1, Potato leafroll virus isolate Villapinzon-bosabita (PLRV Villapinzo): AEB78359.1, Potato leafroll virus isolate Madrid-los arboles2 (PLRV Arboles): AEB78369.1, Potato leafroll virus isolate TZ:LR3M:11 (PLRV TZ:LR3M): AGN48055.1, Potato leafroll virus isolate PLRV171 (PLRV Canada): AYA73300.1, Colombia Potato leafroll virus isolate PLRV165 (PLRV Colombia): AYA73294.1, Tamil nadu Potato leafroll virus isolate OTNI-2 (PLRV Tamilnadu): AFJ11889.1, Jalandgar Potato leafroll virus isolate (PLRV Jalandgar): AFJ11863.1, Karlovce Potato leafroll virus isolate VIRUBRA 1/045 (PLRV VIRUBRA45): ACD93696.1, Australia Potato leafroll virus (PLRV Australia): QBO24572.1, Shimla Potato leafroll virus isolate PBI-6 (PLRV Shimla): AFJ11881.1, Potato leafroll virus (PLRV): QBO24572.1, Prague Potato leafroll virus isolate VIRUBRA 1/046 (PLRV VIRUBRA46): ACD93705.1, Potato leafroll virus isolate Say88 (PLRV Say88): QBO24572.1, Potato leafroll virus isolate Turmeque-siguineque3 (PLRV Siguineque3): AEB78383.1, Potato leafroll virus isolate Facatativa (PLRV Facatat): AEB78357.1, Germany Potato leafroll virus isolate PLRV-DSMZ (PLRV Germany): ADE93559.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentinian): ADE93558.1].
Chromobacterium Pv104, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


All the lectins employed labelled the vomeronasal glands (VG) and the muco-microvillar complex, i.e. the superficial border of both vomeronasal epithelia. A, E . Labelling of the VNO at levels L3 and L8, respectively with the lectin SBA ( Glycine max ). In both cases the VG are strongly labelled. B. VVA ( Vicia villosa ) labelling at a central level L6 of the VNO shows a faint labelling of the VG. C, D. LEA ( Lycopersicon esculentum ) labels the vomeronasal epithelia, the NVNs, and the VG. LEA does not stain the caudal nasal nerve (asterisk in C). F, I, K, N. STA ( Solanum tuberosum ) lectin labelling at central levels (L4, L5) of the VNO stains the vomeronasal epithelia, vomeronasal nerves (NVN) and VG. G, H, J, L, M. UEA ( Ulex europaeus ) lectin stains vomeronasal epithelia, VG, but hardly stains NVNs. Vv, veins; directionality is indicated by the following: d, dorsal; l, lateral; m, medial; v, ventral. Scale bars: A-C, E, I, J = 500 μm; D, F, G = 250 μm; H = 100 μm; K-N = 50 μm.

Journal: bioRxiv

Article Title: Exploring the vomeronasal organ in an endangered antelope species

doi: 10.1101/2023.03.09.531847

Figure Lengend Snippet: All the lectins employed labelled the vomeronasal glands (VG) and the muco-microvillar complex, i.e. the superficial border of both vomeronasal epithelia. A, E . Labelling of the VNO at levels L3 and L8, respectively with the lectin SBA ( Glycine max ). In both cases the VG are strongly labelled. B. VVA ( Vicia villosa ) labelling at a central level L6 of the VNO shows a faint labelling of the VG. C, D. LEA ( Lycopersicon esculentum ) labels the vomeronasal epithelia, the NVNs, and the VG. LEA does not stain the caudal nasal nerve (asterisk in C). F, I, K, N. STA ( Solanum tuberosum ) lectin labelling at central levels (L4, L5) of the VNO stains the vomeronasal epithelia, vomeronasal nerves (NVN) and VG. G, H, J, L, M. UEA ( Ulex europaeus ) lectin stains vomeronasal epithelia, VG, but hardly stains NVNs. Vv, veins; directionality is indicated by the following: d, dorsal; l, lateral; m, medial; v, ventral. Scale bars: A-C, E, I, J = 500 μm; D, F, G = 250 μm; H = 100 μm; K-N = 50 μm.

Article Snippet: STA (Vector-B1165), which is isolated from potato, Solanum tuberosum , binds oligomers of N-acetylglucosamine and some oligosaccharides containing N- acetylglucosamine and N-acetylmuramic acid ( ).

Techniques: Staining

Fig. 1. Callus Tissue with the formation of globular structures

Journal: Proceedings of Universities. Applied Chemistry and Biotechnology

Article Title: Study of the activity of antioxidant enzymes in somaclonal potato variants for use in selection for resistance to the PVS virus

doi: 10.21285/2227-2925-2023-13-3-442-453

Figure Lengend Snippet: Fig. 1. Callus Tissue with the formation of globular structures

Article Snippet: Моделирование биотического стресса (вирус PVS0, DSMZ PV–0838).

Techniques:

Fig. 2. Induction of callus formation in potato stem explants: 1 – morphogenic callus tissue with areas of dense parenchymal and meristematic tissue; 2 – medium-density callus tissue of light yellow color with meristematic zones

Journal: Proceedings of Universities. Applied Chemistry and Biotechnology

Article Title: Study of the activity of antioxidant enzymes in somaclonal potato variants for use in selection for resistance to the PVS virus

doi: 10.21285/2227-2925-2023-13-3-442-453

Figure Lengend Snippet: Fig. 2. Induction of callus formation in potato stem explants: 1 – morphogenic callus tissue with areas of dense parenchymal and meristematic tissue; 2 – medium-density callus tissue of light yellow color with meristematic zones

Article Snippet: Моделирование биотического стресса (вирус PVS0, DSMZ PV–0838).

Techniques:

Fig. 3. Potato regenerated plants obtained from meristematic callus tissue

Journal: Proceedings of Universities. Applied Chemistry and Biotechnology

Article Title: Study of the activity of antioxidant enzymes in somaclonal potato variants for use in selection for resistance to the PVS virus

doi: 10.21285/2227-2925-2023-13-3-442-453

Figure Lengend Snippet: Fig. 3. Potato regenerated plants obtained from meristematic callus tissue

Article Snippet: Моделирование биотического стресса (вирус PVS0, DSMZ PV–0838).

Techniques:

Fig. 4. Infected potato plants with no signs of infection development: V1 – Ermak variety, V2 – Alena variety, V3 – Khozyayushka variety; EC1, AC91, XC94 – somaclonal potato samples

Journal: Proceedings of Universities. Applied Chemistry and Biotechnology

Article Title: Study of the activity of antioxidant enzymes in somaclonal potato variants for use in selection for resistance to the PVS virus

doi: 10.21285/2227-2925-2023-13-3-442-453

Figure Lengend Snippet: Fig. 4. Infected potato plants with no signs of infection development: V1 – Ermak variety, V2 – Alena variety, V3 – Khozyayushka variety; EC1, AC91, XC94 – somaclonal potato samples

Article Snippet: Моделирование биотического стресса (вирус PVS0, DSMZ PV–0838).

Techniques: Infection

Fig. 5. Determination of in gel activity of isoperoxidases in potato leaves, where V1 is Hostess, V2 is Alena, V3 is Ermak are samples extracted from the control group; XC94, AC91, EC1 are samples extracted from somaclonal potato samples. Molecular masses were obtained by processing the electro- phoregram using the BioCapt program (Vilber Lourmat)

Journal: Proceedings of Universities. Applied Chemistry and Biotechnology

Article Title: Study of the activity of antioxidant enzymes in somaclonal potato variants for use in selection for resistance to the PVS virus

doi: 10.21285/2227-2925-2023-13-3-442-453

Figure Lengend Snippet: Fig. 5. Determination of in gel activity of isoperoxidases in potato leaves, where V1 is Hostess, V2 is Alena, V3 is Ermak are samples extracted from the control group; XC94, AC91, EC1 are samples extracted from somaclonal potato samples. Molecular masses were obtained by processing the electro- phoregram using the BioCapt program (Vilber Lourmat)

Article Snippet: Моделирование биотического стресса (вирус PVS0, DSMZ PV–0838).

Techniques: Activity Assay, Control

Fig. 6. Determination of in gel activity of catalase isoforms in potato leaves, where V1 is Hostess, V2 is Alena, V3 is Ermak are samples extracted from the control group; XC94, AC91, EC1 – samples extracted from somaclonal potato samples; Cat1–Cat 4 – catalase isoenzymes

Journal: Proceedings of Universities. Applied Chemistry and Biotechnology

Article Title: Study of the activity of antioxidant enzymes in somaclonal potato variants for use in selection for resistance to the PVS virus

doi: 10.21285/2227-2925-2023-13-3-442-453

Figure Lengend Snippet: Fig. 6. Determination of in gel activity of catalase isoforms in potato leaves, where V1 is Hostess, V2 is Alena, V3 is Ermak are samples extracted from the control group; XC94, AC91, EC1 – samples extracted from somaclonal potato samples; Cat1–Cat 4 – catalase isoenzymes

Article Snippet: Моделирование биотического стресса (вирус PVS0, DSMZ PV–0838).

Techniques: Activity Assay, Control

Fig. 7. Determination of superoxide dismutase forms in potato leaves, where V1 is Hostess, V2 is Alena, V3 is Ermak are samples extracted from the control group; XC94, AC91, EC1 – samples extracted from somaclonal potato samples

Journal: Proceedings of Universities. Applied Chemistry and Biotechnology

Article Title: Study of the activity of antioxidant enzymes in somaclonal potato variants for use in selection for resistance to the PVS virus

doi: 10.21285/2227-2925-2023-13-3-442-453

Figure Lengend Snippet: Fig. 7. Determination of superoxide dismutase forms in potato leaves, where V1 is Hostess, V2 is Alena, V3 is Ermak are samples extracted from the control group; XC94, AC91, EC1 – samples extracted from somaclonal potato samples

Article Snippet: Моделирование биотического стресса (вирус PVS0, DSMZ PV–0838).

Techniques: Control

Fig. 8. Determination of in gel activity of superoxide dismutase forms in potato leaves with inhibitors 3 mM KCN and 5 mM H2O2

Journal: Proceedings of Universities. Applied Chemistry and Biotechnology

Article Title: Study of the activity of antioxidant enzymes in somaclonal potato variants for use in selection for resistance to the PVS virus

doi: 10.21285/2227-2925-2023-13-3-442-453

Figure Lengend Snippet: Fig. 8. Determination of in gel activity of superoxide dismutase forms in potato leaves with inhibitors 3 mM KCN and 5 mM H2O2

Article Snippet: Моделирование биотического стресса (вирус PVS0, DSMZ PV–0838).

Techniques: Activity Assay

Figure 1. (A) Sequence alignments of the ATPase domain of PLRV_MP. Multiple sequence alignments, generated using ClustalW, were manually corrected for domain superimpositions. The number(s) in brackets represent the number of amino acids. (B) Organization of functional motifs on the polypeptide chain of MP of PLRV. Schematics are drawn approximately to the scale and represent the approximate consensus of representative homologs. (C) I-TASSER predicted atomic model of PLRV_MP with all the putative ATP catalysis motifs. [Accession Numbers: Potato leafroll virus isolate PLRV-JF (PLRV JF): ADE93562.1, Potato leafroll virus isolate Anoquia (PLRV Antioquia): QES86451.1, Potato leafroll virus isolate Sta Rosa de Osos-santana (PLRV Santana): AEB78381.1, Potato leafroll virus isolate Turmeque-siguineque (PLRV Tur-sig): AEB78353.1, Potato leafroll virus isolate Sonson-tasajo (PLRV Sonson): AEB78367.1, Potato leafroll virus isolate Siachoque-jurubita (PLRV Jurubita): AEB78379.1, Potato leafroll virus isolate Pasto-el campanero2 (PLRV Pasto-el): AEB78377.1, Potato leafroll virus isolate Pasto-el campanero (PLRV Campa): AEB78375.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentina): ADE93558.1, Potato leafroll virus isolate Zipaquira-san Jorge (PLRV Zipaquir): AEB78361.1, Potato leafroll virus isolate Turmeque-siguineque2 (PLRV Turmeque): AEB78373.1, Potato leafroll virus isolate Ipiales-suras (PLRV Suras): AEB78385.1, France Potato leafroll virus strain CIP01 (PLRV CIP01): AAL77949.1, Potato leafroll virus isolate PLRV184 (PLRV 184): AYA73306.1, Potato leafroll virus isolate Villapinzon-bosabita (PLRV Villapinzo): AEB78359.1, Potato leafroll virus isolate Madrid-los arboles2 (PLRV Arboles): AEB78369.1, Potato leafroll virus isolate TZ:LR3M:11 (PLRV TZ:LR3M): AGN48055.1, Potato leafroll virus isolate PLRV171 (PLRV Canada): AYA73300.1, Colombia Potato leafroll virus isolate PLRV165 (PLRV Colombia): AYA73294.1, Tamil nadu Potato leafroll virus isolate OTNI-2 (PLRV Tamilnadu): AFJ11889.1, Jalandgar Potato leafroll virus isolate (PLRV Jalandgar): AFJ11863.1, Karlovce Potato leafroll virus isolate VIRUBRA 1/045 (PLRV VIRUBRA45): ACD93696.1, Australia Potato leafroll virus (PLRV Australia): QBO24572.1, Shimla Potato leafroll virus isolate PBI-6 (PLRV Shimla): AFJ11881.1, Potato leafroll virus (PLRV): QBO24572.1, Prague Potato leafroll virus isolate VIRUBRA 1/046 (PLRV VIRUBRA46): ACD93705.1, Potato leafroll virus isolate Say88 (PLRV Say88): QBO24572.1, Potato leafroll virus isolate Turmeque-siguineque3 (PLRV Siguineque3): AEB78383.1, Potato leafroll virus isolate Facatativa (PLRV Facatat): AEB78357.1, Germany Potato leafroll virus isolate PLRV-DSMZ (PLRV Germany): ADE93559.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentinian): ADE93558.1].

Journal: Scientific reports

Article Title: Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein.

doi: 10.1038/s41598-020-78791-4

Figure Lengend Snippet: Figure 1. (A) Sequence alignments of the ATPase domain of PLRV_MP. Multiple sequence alignments, generated using ClustalW, were manually corrected for domain superimpositions. The number(s) in brackets represent the number of amino acids. (B) Organization of functional motifs on the polypeptide chain of MP of PLRV. Schematics are drawn approximately to the scale and represent the approximate consensus of representative homologs. (C) I-TASSER predicted atomic model of PLRV_MP with all the putative ATP catalysis motifs. [Accession Numbers: Potato leafroll virus isolate PLRV-JF (PLRV JF): ADE93562.1, Potato leafroll virus isolate Anoquia (PLRV Antioquia): QES86451.1, Potato leafroll virus isolate Sta Rosa de Osos-santana (PLRV Santana): AEB78381.1, Potato leafroll virus isolate Turmeque-siguineque (PLRV Tur-sig): AEB78353.1, Potato leafroll virus isolate Sonson-tasajo (PLRV Sonson): AEB78367.1, Potato leafroll virus isolate Siachoque-jurubita (PLRV Jurubita): AEB78379.1, Potato leafroll virus isolate Pasto-el campanero2 (PLRV Pasto-el): AEB78377.1, Potato leafroll virus isolate Pasto-el campanero (PLRV Campa): AEB78375.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentina): ADE93558.1, Potato leafroll virus isolate Zipaquira-san Jorge (PLRV Zipaquir): AEB78361.1, Potato leafroll virus isolate Turmeque-siguineque2 (PLRV Turmeque): AEB78373.1, Potato leafroll virus isolate Ipiales-suras (PLRV Suras): AEB78385.1, France Potato leafroll virus strain CIP01 (PLRV CIP01): AAL77949.1, Potato leafroll virus isolate PLRV184 (PLRV 184): AYA73306.1, Potato leafroll virus isolate Villapinzon-bosabita (PLRV Villapinzo): AEB78359.1, Potato leafroll virus isolate Madrid-los arboles2 (PLRV Arboles): AEB78369.1, Potato leafroll virus isolate TZ:LR3M:11 (PLRV TZ:LR3M): AGN48055.1, Potato leafroll virus isolate PLRV171 (PLRV Canada): AYA73300.1, Colombia Potato leafroll virus isolate PLRV165 (PLRV Colombia): AYA73294.1, Tamil nadu Potato leafroll virus isolate OTNI-2 (PLRV Tamilnadu): AFJ11889.1, Jalandgar Potato leafroll virus isolate (PLRV Jalandgar): AFJ11863.1, Karlovce Potato leafroll virus isolate VIRUBRA 1/045 (PLRV VIRUBRA45): ACD93696.1, Australia Potato leafroll virus (PLRV Australia): QBO24572.1, Shimla Potato leafroll virus isolate PBI-6 (PLRV Shimla): AFJ11881.1, Potato leafroll virus (PLRV): QBO24572.1, Prague Potato leafroll virus isolate VIRUBRA 1/046 (PLRV VIRUBRA46): ACD93705.1, Potato leafroll virus isolate Say88 (PLRV Say88): QBO24572.1, Potato leafroll virus isolate Turmeque-siguineque3 (PLRV Siguineque3): AEB78383.1, Potato leafroll virus isolate Facatativa (PLRV Facatat): AEB78357.1, Germany Potato leafroll virus isolate PLRV-DSMZ (PLRV Germany): ADE93559.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentinian): ADE93558.1].

Article Snippet: [Accession Numbers: Potato leafroll virus isolate PLRV-JF (PLRV JF): ADE93562.1, Potato leafroll virus isolate Anoquia (PLRV Antioquia): QES86451.1, Potato leafroll virus isolate Sta Rosa de Osos-santana (PLRV Santana): AEB78381.1, Potato leafroll virus isolate Turmeque-siguineque (PLRV Tur-sig): AEB78353.1, Potato leafroll virus isolate Sonson-tasajo (PLRV Sonson): AEB78367.1, Potato leafroll virus isolate Siachoque-jurubita (PLRV Jurubita): AEB78379.1, Potato leafroll virus isolate Pasto-el campanero2 (PLRV Pasto-el): AEB78377.1, Potato leafroll virus isolate Pasto-el campanero (PLRV Campa): AEB78375.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentina): ADE93558.1, Potato leafroll virus isolate Zipaquira-san Jorge (PLRV Zipaquir): AEB78361.1, Potato leafroll virus isolate Turmeque-siguineque2 (PLRV Turmeque): AEB78373.1, Potato leafroll virus isolate Ipiales-suras (PLRV Suras): AEB78385.1, France Potato leafroll virus strain CIP01 (PLRV CIP01): AAL77949.1, Potato leafroll virus isolate PLRV184 (PLRV 184): AYA73306.1, Potato leafroll virus isolate Villapinzon-bosabita (PLRV Villapinzo): AEB78359.1, Potato leafroll virus isolate Madrid-los arboles2 (PLRV Arboles): AEB78369.1, Potato leafroll virus isolate TZ:LR3M:11 (PLRV TZ:LR3M): AGN48055.1, Potato leafroll virus isolate PLRV171 (PLRV Canada): AYA73300.1, Colombia Potato leafroll virus isolate PLRV165 (PLRV Colombia): AYA73294.1, Tamil nadu Potato leafroll virus isolate OTNI-2 (PLRV Tamilnadu): AFJ11889.1, Jalandgar Potato leafroll virus isolate (PLRV Jalandgar): AFJ11863.1, Karlovce Potato leafroll virus isolate VIRUBRA 1/045 (PLRV VIRUBRA45): ACD93696.1, Australia Potato leafroll virus (PLRV Australia): QBO24572.1, Shimla Potato leafroll virus isolate PBI-6 (PLRV Shimla): AFJ11881.1, Potato leafroll virus (PLRV): QBO24572.1, Prague Potato leafroll virus isolate VIRUBRA 1/046 (PLRV VIRUBRA46): ACD93705.1, Potato leafroll virus isolate Say88 (PLRV Say88): QBO24572.1, Potato leafroll virus isolate Turmeque-siguineque3 (PLRV Siguineque3): AEB78383.1, Potato leafroll virus isolate Facatativa (PLRV Facatat): AEB78357.1, Germany Potato leafroll virus isolate PLRV-DSMZ (PLRV Germany): ADE93559.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentinian): ADE93558.1].

Techniques: Sequencing, Generated, Functional Assay, Virus

Figure 2. (A) SDS-PAGE analysis of affinity purified his-tagged PLRV_MP expressed in E. coli. Lane 1: Purified protein and lane 2: Marker. (B) Purified MP exhibits basal and DNA-stimulated ATPase activity. (C) Apart from this, MP also exhibits RNA stimulated ATPase activity. Protein concentrations were 2 μM and 10 μM. Only protein is indicated with light grey and protein + dsDNA is indicated with dark grey. The vertical bars represent the mean values of three replicates (n = 3) and the values are highly significant exhibited at 0.001 level. DNA/ RNA concentration was maintained constant at 40 ng for every independent reaction. Negative controls with only buffer were also taken into account while performing the reactions.

Journal: Scientific reports

Article Title: Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein.

doi: 10.1038/s41598-020-78791-4

Figure Lengend Snippet: Figure 2. (A) SDS-PAGE analysis of affinity purified his-tagged PLRV_MP expressed in E. coli. Lane 1: Purified protein and lane 2: Marker. (B) Purified MP exhibits basal and DNA-stimulated ATPase activity. (C) Apart from this, MP also exhibits RNA stimulated ATPase activity. Protein concentrations were 2 μM and 10 μM. Only protein is indicated with light grey and protein + dsDNA is indicated with dark grey. The vertical bars represent the mean values of three replicates (n = 3) and the values are highly significant exhibited at 0.001 level. DNA/ RNA concentration was maintained constant at 40 ng for every independent reaction. Negative controls with only buffer were also taken into account while performing the reactions.

Article Snippet: [Accession Numbers: Potato leafroll virus isolate PLRV-JF (PLRV JF): ADE93562.1, Potato leafroll virus isolate Anoquia (PLRV Antioquia): QES86451.1, Potato leafroll virus isolate Sta Rosa de Osos-santana (PLRV Santana): AEB78381.1, Potato leafroll virus isolate Turmeque-siguineque (PLRV Tur-sig): AEB78353.1, Potato leafroll virus isolate Sonson-tasajo (PLRV Sonson): AEB78367.1, Potato leafroll virus isolate Siachoque-jurubita (PLRV Jurubita): AEB78379.1, Potato leafroll virus isolate Pasto-el campanero2 (PLRV Pasto-el): AEB78377.1, Potato leafroll virus isolate Pasto-el campanero (PLRV Campa): AEB78375.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentina): ADE93558.1, Potato leafroll virus isolate Zipaquira-san Jorge (PLRV Zipaquir): AEB78361.1, Potato leafroll virus isolate Turmeque-siguineque2 (PLRV Turmeque): AEB78373.1, Potato leafroll virus isolate Ipiales-suras (PLRV Suras): AEB78385.1, France Potato leafroll virus strain CIP01 (PLRV CIP01): AAL77949.1, Potato leafroll virus isolate PLRV184 (PLRV 184): AYA73306.1, Potato leafroll virus isolate Villapinzon-bosabita (PLRV Villapinzo): AEB78359.1, Potato leafroll virus isolate Madrid-los arboles2 (PLRV Arboles): AEB78369.1, Potato leafroll virus isolate TZ:LR3M:11 (PLRV TZ:LR3M): AGN48055.1, Potato leafroll virus isolate PLRV171 (PLRV Canada): AYA73300.1, Colombia Potato leafroll virus isolate PLRV165 (PLRV Colombia): AYA73294.1, Tamil nadu Potato leafroll virus isolate OTNI-2 (PLRV Tamilnadu): AFJ11889.1, Jalandgar Potato leafroll virus isolate (PLRV Jalandgar): AFJ11863.1, Karlovce Potato leafroll virus isolate VIRUBRA 1/045 (PLRV VIRUBRA45): ACD93696.1, Australia Potato leafroll virus (PLRV Australia): QBO24572.1, Shimla Potato leafroll virus isolate PBI-6 (PLRV Shimla): AFJ11881.1, Potato leafroll virus (PLRV): QBO24572.1, Prague Potato leafroll virus isolate VIRUBRA 1/046 (PLRV VIRUBRA46): ACD93705.1, Potato leafroll virus isolate Say88 (PLRV Say88): QBO24572.1, Potato leafroll virus isolate Turmeque-siguineque3 (PLRV Siguineque3): AEB78383.1, Potato leafroll virus isolate Facatativa (PLRV Facatat): AEB78357.1, Germany Potato leafroll virus isolate PLRV-DSMZ (PLRV Germany): ADE93559.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentinian): ADE93558.1].

Techniques: SDS Page, Affinity Purification, Purification, Marker, Activity Assay, Concentration Assay

Figure 3. Reverse-transcription polymerase chain reaction (RT-PCR) was performed to amplify sense and antisense PLRV_MP sequences from the leaves of infected potato plants. (A) Amplified cDNA fragments were analyzed by electrophoresis on 0.8% agarose gel. M: 100 bp DNA ladder; lanes 1 and 2: PCR products of sense and antisense MP (471 bp). (B) The pHANNIBAL plasmids were purified from E. coli DH5a cultures and analyzed by electrophoresis on 0.8% agarose gel. M: 1 kb DNA ladder; lanes 1: pHANNIBAL plasmid (5.83 kb). (C) The pART27 binary plasmid was purified from E. coli DH5a culture and analyzed by electrophoresis on 0.8% agarose gel. M: 1 kb DNA ladder; lane 1: pART27 plasmid (11.6 kb). (D) Confirmation of siRNA constructs in pHANNIBAL and pART27 binary vector through restriction analysis. M: 1 kb DNA ladder; lane 1: undigested pHANNIBAL; lane 2: antisense MP ligated with pHANNIBAL (pHANNIBAL-antisense MP construct); lane 3: both antisense and sense MP ligated with pHANNIBAL (pHANNIBAL-MP-siRNA construct); lane 4: HindIII and BamHI digestion of pHANNIBAL- antisense MP construct shows the release of a 471 bp fragment; lane 5: XhoI and KpnI digestion of pHANNIBAL-MP siRNA construct also shows the release of 471 bp fragments; lane 6: XhoI and BamHI digestion of pHANNIBAL-MP siRNA construct shows the release of a ~ 1500 bp fragment; and lane 7: NotI digestion of pHANNIBAL-MP siRNA construct shows the release of two fragments of ~ 4 kb (including sense MP, intron, and antisense MP sequence) and 3.5 kb. (E) The whole siRNA cassette (~ 4 kb) was transferred from pHANNIBAL to pART27, and this was further confirmed by NotI restriction analysis. (F) Schematic representation of pART27-MP siRNA constructs used for transient expression by agroinfiltration.

Journal: Scientific reports

Article Title: Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein.

doi: 10.1038/s41598-020-78791-4

Figure Lengend Snippet: Figure 3. Reverse-transcription polymerase chain reaction (RT-PCR) was performed to amplify sense and antisense PLRV_MP sequences from the leaves of infected potato plants. (A) Amplified cDNA fragments were analyzed by electrophoresis on 0.8% agarose gel. M: 100 bp DNA ladder; lanes 1 and 2: PCR products of sense and antisense MP (471 bp). (B) The pHANNIBAL plasmids were purified from E. coli DH5a cultures and analyzed by electrophoresis on 0.8% agarose gel. M: 1 kb DNA ladder; lanes 1: pHANNIBAL plasmid (5.83 kb). (C) The pART27 binary plasmid was purified from E. coli DH5a culture and analyzed by electrophoresis on 0.8% agarose gel. M: 1 kb DNA ladder; lane 1: pART27 plasmid (11.6 kb). (D) Confirmation of siRNA constructs in pHANNIBAL and pART27 binary vector through restriction analysis. M: 1 kb DNA ladder; lane 1: undigested pHANNIBAL; lane 2: antisense MP ligated with pHANNIBAL (pHANNIBAL-antisense MP construct); lane 3: both antisense and sense MP ligated with pHANNIBAL (pHANNIBAL-MP-siRNA construct); lane 4: HindIII and BamHI digestion of pHANNIBAL- antisense MP construct shows the release of a 471 bp fragment; lane 5: XhoI and KpnI digestion of pHANNIBAL-MP siRNA construct also shows the release of 471 bp fragments; lane 6: XhoI and BamHI digestion of pHANNIBAL-MP siRNA construct shows the release of a ~ 1500 bp fragment; and lane 7: NotI digestion of pHANNIBAL-MP siRNA construct shows the release of two fragments of ~ 4 kb (including sense MP, intron, and antisense MP sequence) and 3.5 kb. (E) The whole siRNA cassette (~ 4 kb) was transferred from pHANNIBAL to pART27, and this was further confirmed by NotI restriction analysis. (F) Schematic representation of pART27-MP siRNA constructs used for transient expression by agroinfiltration.

Article Snippet: [Accession Numbers: Potato leafroll virus isolate PLRV-JF (PLRV JF): ADE93562.1, Potato leafroll virus isolate Anoquia (PLRV Antioquia): QES86451.1, Potato leafroll virus isolate Sta Rosa de Osos-santana (PLRV Santana): AEB78381.1, Potato leafroll virus isolate Turmeque-siguineque (PLRV Tur-sig): AEB78353.1, Potato leafroll virus isolate Sonson-tasajo (PLRV Sonson): AEB78367.1, Potato leafroll virus isolate Siachoque-jurubita (PLRV Jurubita): AEB78379.1, Potato leafroll virus isolate Pasto-el campanero2 (PLRV Pasto-el): AEB78377.1, Potato leafroll virus isolate Pasto-el campanero (PLRV Campa): AEB78375.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentina): ADE93558.1, Potato leafroll virus isolate Zipaquira-san Jorge (PLRV Zipaquir): AEB78361.1, Potato leafroll virus isolate Turmeque-siguineque2 (PLRV Turmeque): AEB78373.1, Potato leafroll virus isolate Ipiales-suras (PLRV Suras): AEB78385.1, France Potato leafroll virus strain CIP01 (PLRV CIP01): AAL77949.1, Potato leafroll virus isolate PLRV184 (PLRV 184): AYA73306.1, Potato leafroll virus isolate Villapinzon-bosabita (PLRV Villapinzo): AEB78359.1, Potato leafroll virus isolate Madrid-los arboles2 (PLRV Arboles): AEB78369.1, Potato leafroll virus isolate TZ:LR3M:11 (PLRV TZ:LR3M): AGN48055.1, Potato leafroll virus isolate PLRV171 (PLRV Canada): AYA73300.1, Colombia Potato leafroll virus isolate PLRV165 (PLRV Colombia): AYA73294.1, Tamil nadu Potato leafroll virus isolate OTNI-2 (PLRV Tamilnadu): AFJ11889.1, Jalandgar Potato leafroll virus isolate (PLRV Jalandgar): AFJ11863.1, Karlovce Potato leafroll virus isolate VIRUBRA 1/045 (PLRV VIRUBRA45): ACD93696.1, Australia Potato leafroll virus (PLRV Australia): QBO24572.1, Shimla Potato leafroll virus isolate PBI-6 (PLRV Shimla): AFJ11881.1, Potato leafroll virus (PLRV): QBO24572.1, Prague Potato leafroll virus isolate VIRUBRA 1/046 (PLRV VIRUBRA46): ACD93705.1, Potato leafroll virus isolate Say88 (PLRV Say88): QBO24572.1, Potato leafroll virus isolate Turmeque-siguineque3 (PLRV Siguineque3): AEB78383.1, Potato leafroll virus isolate Facatativa (PLRV Facatat): AEB78357.1, Germany Potato leafroll virus isolate PLRV-DSMZ (PLRV Germany): ADE93559.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentinian): ADE93558.1].

Techniques: Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Infection, Amplification, Electrophoresis, Agarose Gel Electrophoresis, Purification, Plasmid Preparation, Construct, Sequencing, Expressing

Figure 4. (A) Symptoms observed in the tertiary leaves of the PLRV infected potato plants. (1) PLRV infected control without agroinfiltration; (2) agroinfiltrated with the empty vector pART27; (3) agroinfiltrated with the plasmid containing only antisense sequence (pART27-antisense MP); (4) agroinfiltrated with the plasmid containing only sense sequence (pART27-sense MP); (5) agroinfiltrated with the pART27-MP siRNA construct; (B) Confirmation of expression of siRNA using Northern blotting analysis. Higher expression of siRNA in the leaves at 10 dpi (lane 1) was observed as compared to 5 dpi (lane 2), whereas empty vector (mock) agro-infected leaves did not show any siRNA (lane 3). (C) Detection of PLRV RNA by RT-PCR. Amplified cDNA fragments (627 bp) were analyzed by electrophoresis on 0.8% agarose gel. M: 100 bp marker; lane 1: PLRV was observed in control plant; lane 2: PLRV from tertiary leaves of the plant containing the empty vector pART26; lane 3: PLRV from tertiary leaves of the plant containing the antisense construct (pART27-antisense MP); lane 4: PLRV from tertiary leaves of the plant containing the sense construct (pART27-sense MP); lane 5: no PLRV in tertiary leaves of the plant containing MP siRNA (pART27-MP). (D) Actin PCR was performed as an internal control.

Journal: Scientific reports

Article Title: Inhibition of potato leafroll virus multiplication and systemic translocation by siRNA constructs against putative ATPase fold of movement protein.

doi: 10.1038/s41598-020-78791-4

Figure Lengend Snippet: Figure 4. (A) Symptoms observed in the tertiary leaves of the PLRV infected potato plants. (1) PLRV infected control without agroinfiltration; (2) agroinfiltrated with the empty vector pART27; (3) agroinfiltrated with the plasmid containing only antisense sequence (pART27-antisense MP); (4) agroinfiltrated with the plasmid containing only sense sequence (pART27-sense MP); (5) agroinfiltrated with the pART27-MP siRNA construct; (B) Confirmation of expression of siRNA using Northern blotting analysis. Higher expression of siRNA in the leaves at 10 dpi (lane 1) was observed as compared to 5 dpi (lane 2), whereas empty vector (mock) agro-infected leaves did not show any siRNA (lane 3). (C) Detection of PLRV RNA by RT-PCR. Amplified cDNA fragments (627 bp) were analyzed by electrophoresis on 0.8% agarose gel. M: 100 bp marker; lane 1: PLRV was observed in control plant; lane 2: PLRV from tertiary leaves of the plant containing the empty vector pART26; lane 3: PLRV from tertiary leaves of the plant containing the antisense construct (pART27-antisense MP); lane 4: PLRV from tertiary leaves of the plant containing the sense construct (pART27-sense MP); lane 5: no PLRV in tertiary leaves of the plant containing MP siRNA (pART27-MP). (D) Actin PCR was performed as an internal control.

Article Snippet: [Accession Numbers: Potato leafroll virus isolate PLRV-JF (PLRV JF): ADE93562.1, Potato leafroll virus isolate Anoquia (PLRV Antioquia): QES86451.1, Potato leafroll virus isolate Sta Rosa de Osos-santana (PLRV Santana): AEB78381.1, Potato leafroll virus isolate Turmeque-siguineque (PLRV Tur-sig): AEB78353.1, Potato leafroll virus isolate Sonson-tasajo (PLRV Sonson): AEB78367.1, Potato leafroll virus isolate Siachoque-jurubita (PLRV Jurubita): AEB78379.1, Potato leafroll virus isolate Pasto-el campanero2 (PLRV Pasto-el): AEB78377.1, Potato leafroll virus isolate Pasto-el campanero (PLRV Campa): AEB78375.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentina): ADE93558.1, Potato leafroll virus isolate Zipaquira-san Jorge (PLRV Zipaquir): AEB78361.1, Potato leafroll virus isolate Turmeque-siguineque2 (PLRV Turmeque): AEB78373.1, Potato leafroll virus isolate Ipiales-suras (PLRV Suras): AEB78385.1, France Potato leafroll virus strain CIP01 (PLRV CIP01): AAL77949.1, Potato leafroll virus isolate PLRV184 (PLRV 184): AYA73306.1, Potato leafroll virus isolate Villapinzon-bosabita (PLRV Villapinzo): AEB78359.1, Potato leafroll virus isolate Madrid-los arboles2 (PLRV Arboles): AEB78369.1, Potato leafroll virus isolate TZ:LR3M:11 (PLRV TZ:LR3M): AGN48055.1, Potato leafroll virus isolate PLRV171 (PLRV Canada): AYA73300.1, Colombia Potato leafroll virus isolate PLRV165 (PLRV Colombia): AYA73294.1, Tamil nadu Potato leafroll virus isolate OTNI-2 (PLRV Tamilnadu): AFJ11889.1, Jalandgar Potato leafroll virus isolate (PLRV Jalandgar): AFJ11863.1, Karlovce Potato leafroll virus isolate VIRUBRA 1/045 (PLRV VIRUBRA45): ACD93696.1, Australia Potato leafroll virus (PLRV Australia): QBO24572.1, Shimla Potato leafroll virus isolate PBI-6 (PLRV Shimla): AFJ11881.1, Potato leafroll virus (PLRV): QBO24572.1, Prague Potato leafroll virus isolate VIRUBRA 1/046 (PLRV VIRUBRA46): ACD93705.1, Potato leafroll virus isolate Say88 (PLRV Say88): QBO24572.1, Potato leafroll virus isolate Turmeque-siguineque3 (PLRV Siguineque3): AEB78383.1, Potato leafroll virus isolate Facatativa (PLRV Facatat): AEB78357.1, Germany Potato leafroll virus isolate PLRV-DSMZ (PLRV Germany): ADE93559.1, Argentinian Potato leafroll virus isolate PLRV-5 (PLRV Argentinian): ADE93558.1].

Techniques: Infection, Control, Plasmid Preparation, Sequencing, Construct, Expressing, Northern Blot, Reverse Transcription Polymerase Chain Reaction, Amplification, Electrophoresis, Agarose Gel Electrophoresis, Marker