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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: α-Thrombin Rapidly Induces Tyrosine Phosphorylation of a Novel, 74–78-kDa Stress Response Protein(s) in Lung Fibroblast Cells
doi: 10.1074/jbc.m409043200
Figure Lengend Snippet: FIG. 4. The p74–78-kDa protein is localized to the cytoplasm. A, serum-starved cells were untreated or treated -thrombin (0.4 units/ml; 15 min) and lysed, and then membrane, cytoplasm, and nuclear frac- tions were prepared as described under “Experimental Procedures.” 15 g of the sample were run on an 8% SDS-polyacrylamide gel, trans- ferred to nitrocellulose, and immunoblotted with the phospho-specific anti-Stat3 antibody. The positions of three distinct bands (74–78 kDa) are indicated by arrows. B and C are blots probed with anti-Stat3 antibody and anti-TGF- receptor antibody, respectively. These blots are representative of three independent experiments. Th, -thrombin; CR, cross-reactive.
Article Snippet: IL-6, PDGF, transforming growth factor- (TGF- ), and basic fibroblast growth factor (FGF) were obtained from R & D Systems;
Techniques: Membrane