Journal: International Journal of Molecular Sciences

Article Title: Comparative Study of Nanoparticle Blood Circulation after Forced Clearance of Own Erythrocytes (Mononuclear Phagocyte System-Cytoblockade) or Administration of Cytotoxic Doxorubicin- or Clodronate-Loaded Liposomes

doi: 10.3390/ijms241310623

Figure Lengend Snippet: Measurements of the circulation time of MNPs in the bloodstream using the MPQ method. ( a – c ) Representative circulation kinetics for Estapor carboxylated polystyrene magnetic beads only and 24 h after administration of 30 and 240 µg Dox-loaded liposomes. Black dots correspond to the magnetic signal in a range of 0.9–0.1, and grey corresponds to ranges of 1.0–0.9 and 0.1–0. Green lines are the exponential fitting curves for 0.9–0.1 data points that were used to calculate the circulation half-life time (t 1/2 ). ( d ) Half-life times of Estapor carboxylated polystyrene magnetic beads in a bloodstream: 24 h after injection of doxorubicin (Dox)-loaded liposomes, liposomes without Dox, and Dox; 12 h after injection of anti-mouse RBC antibodies; 1 h after injection of SMOFlipid; and 48 h post-injection of clodronic acid (CA)-loaded liposomes. ( e ) Half-life times of different magnetic nanoparticles in the bloodstream after injection of Dox-loaded liposomes. Control mice were injected with MNPs only. The number of animals was at least n = 5 for each group. Statistical significance for different circulation prolongation methods relative to the negative control (corresponding to MNPs only) is shown in black ( d , e ), while statistical significance in comparison to the 240 µg Dox-loaded liposomes is shown in blue. A p -value ≤ 0.05 was considered to be statistically significant (ns— p > 0.05; *— p ≤ 0.05; **— p ≤ 0.01; ***— p ≤ 0.001).

Article Snippet: Estapor tosyl-activated polystyrene magnetic beads , Merck Millipore, USA , 957.2 ± 150.2 , 15.1 ± 0.6.

Techniques: Magnetic Beads, Liposomes, Injection, Control, Negative Control, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Comparative Study of Nanoparticle Blood Circulation after Forced Clearance of Own Erythrocytes (Mononuclear Phagocyte System-Cytoblockade) or Administration of Cytotoxic Doxorubicin- or Clodronate-Loaded Liposomes

doi: 10.3390/ijms241310623

Figure Lengend Snippet: Study of the biodistribution of nanoparticles. Representative merged brightfield and Cy5 or Cy7.5 channel images revealing ( a ) in vivo and ( b ) ex vivo biodistribution of MNPs (Estapor carboxylated polystyrene magnetic beads) and Dox-loaded liposomes. Mice were injected i.v. with Cy7.5-labeled Dox-loaded liposomes 24 h before injection of Cy5-labeled magnetic nanoparticles, or injected separately with Cy7.5-labeled Dox-loaded liposomes or Cy5-labeled MNPs. Non-injected mice were used as control. ( c ) Analysis of MNP biodistribution using the MPQ method with Estapor carboxylated polystyrene magnetic beads only, and after administration of 240 µg Dox-loaded liposomes and anti-mouse RBC antibodies. The number of animals was n = 3 for each group. Statistical significance for different circulation prolongation methods is relative to the negative control (corresponding MNPs only). A p -value of ≤ 0.05 was considered to be statistically significant (ns— p > 0.05; *— p ≤ 0.05; **— p ≤ 0.01; ***— p ≤ 0.001.

Article Snippet: Estapor tosyl-activated polystyrene magnetic beads , Merck Millipore, USA , 957.2 ± 150.2 , 15.1 ± 0.6.

Techniques: In Vivo, Ex Vivo, Magnetic Beads, Liposomes, Injection, Labeling, Control, Negative Control