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Image Search Results
Journal: Oxidative medicine and cellular longevity
Article Title: Inhibition of α -Synuclein Accumulation Improves Neuronal Apoptosis and Delayed Postoperative Cognitive Recovery in Aged Mice.
doi: 10.1155/2021/5572899
Figure Lengend Snippet: Figure 3: Peripheral surgery increased mitochondrial α-syn accumulation and damage. (a) The hippocampus was stained to determine colocalization of α-syn (red) and COXIV (an inner mitochondrial membrane (green)). Cell nuclei were stained with DAPI (blue). Arrows point to mitochondrial α-syn; scale bars = 10 μm, n = 5. (b) Transmission electron microscopy showing mitochondrial morphology in the hippocampus after surgery (n = 3 mice per group) and assessment of abnormal cristae (n = 57 −60 cells). Scale bars = 500 nm. Results are presented as mean ± SEM (one-way analysis of variance). ∗∗∗P < 0:001, control (Con) or anesthesia-alone (Ane) vs. surgery plus anesthesia (Sur).
Article Snippet: Accumulation of SNCA oligomers was detected by immunohistochemistry using a rabbit polyclonal SNCA oligomer-specific Syn33 antibody (ABN2265; Merck Millipore, Billerica, MA, USA), a mouse monoclonal antibody against total SNCA (4D6, ab1903; Abcam), a
Techniques: Staining, Membrane, Transmission Assay, Electron Microscopy, Control
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Article Title: Pantoprazole pretreatment elevates sensitivity to vincristine in drug-resistant oral epidermoid carcinoma in vitro and in vivo.
doi: 10.1016/j.biopha.2019.109478
Figure Lengend Snippet: Fig. 6. PPZ treatment induces an increase in lysosomal pH and inhibits the activity of lysosomal enzyme acid phosphatase in KB/V cells. KB/V cells were treated with various concentrations of PPZ (a) or V-ATPase inhibitor Baf-A1 (b) for 4–24 h, and then the supernatant was harvested for pHe measurements. c Effect of PPZ on lysosomal pH in KB/V cells was evaluated by LysoSensor Green probe using fluorescence microscope. d The activity of lysosomal enzyme acid phosphatase in KB/V cells treated with various concentrations of PPZ for 24 h. e Effect of PPZ on the expression of V-ATPase in KB/V cells was detected and quantified using Western blotting analysis. For a, b, d and e, data are presented as the mean ± SD of three separate experiments. *P < 0.05, **P < 0.01 vs. KB/V control group. f Effect of V-ATPase inhibitor Baf-A1 on the synergistic cytotoxicity of PPZ and VCR in KB/V cells. KB/V cells were treated with Baf-A1 (50, 200 nM) with or without PPZ and VCR, then the cell viability was detected by MTT assay. Data are presented as the mean ± SD from three separate experiments. *P < 0.05, **P < 0.01 vs. KB/V control group; △△P < 0.01 vs. PPZ alone group; ##P < 0.01 vs. PPZ and VCR combination group (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).
Article Snippet: Membranes were blocked in 5% (w/v) non-fat milk before incubation with primary antibodies (1:1000 in primary antibody diluent) against PARP (#9532), cleavedcaspase 3 (#9664), Bcl-xL(#2762), Bcl-2 (#2872), Bax (#2772), p21 (#2947), Cyclin B1 (#4138), cdc2 (#9112), p-cdc2 (#9111), MMP2 (#4022), MMP9 (#3852), P-gp (#13342), EGFR (#2232), p-EGFR (#2235), p-ERK (#4370), p-p38 (#4511), PI3K (#3358), Akt (#2965), p-Akt (#2965), mTOR (#2972), p-mTOR (#2971), β-actin (#4970) (all from Cell Signaling Technology, CST, Boston, MA) and
Techniques: Activity Assay, Microscopy, Expressing, Western Blot, Control, MTT Assay