Journal: Cell Reports Medicine

Article Title: Enhanced TLR3 responsiveness in hepatitis C virus resistant women from the Irish anti-D cohort

doi: 10.1016/j.xcrm.2022.100804

Figure Lengend Snippet: Innate immune stimuli induce changes in gene expression in whole blood from all donors Whole blood from ESN (n = 18), SR (n = 19) and SVR (n = 17) women was stimulated with a panel of antiviral agonists (R848, polyIC, IFNα2). (A) Overview of experimental workflow. (B) Principal component analysis plot of log 2 normalized NanoString transcriptomic data. The percentage variance captured in PC1 and PC2 are indicated in brackets. Each point on the plot represents a single donor for the color denoted stimulation. (C) Heatmap of NanoString transcriptomic data (n = 50 genes) showing negative control (NC) and stimuli induced genes for IFNα2, polyIC, and R848.

Article Snippet: PolyIC TruCulture tube , Myriad RBM , ref. 782-001282.

Techniques: Gene Expression, Negative Control

Journal: Cell Reports Medicine

Article Title: Enhanced TLR3 responsiveness in hepatitis C virus resistant women from the Irish anti-D cohort

doi: 10.1016/j.xcrm.2022.100804

Figure Lengend Snippet: PolyIC-induced higher IFN-I responses in ESN women Fresh whole blood was stimulated with IFNα2, R848, or polyIC for 22 h at 37°C and changes in gene expression assayed using NanoString transcriptomics. (A–C) Heatmaps showing the top 50 genes with the lowest q values when comparing the response with stimulation between ESN and SP donors. (A) IFNα2, p < 0.16, q value (FDR-adjusted p value) < 0.99 (B) R848, p < 0.15, q < 0.99, and (C) polyIC, p < 0.06, q < 0.65. (D–F) MIC-derived IFN-I score in ESN and SP donors after stimulation with (D) IFNα2 (E) R848, and (F) polyIC (∗p < 0.05). Data are presented with median line reported as a solid line. Comparisons between ESNs and SPs were made using Mann-Whitney U tests (∗p < 0.05).

Article Snippet: PolyIC TruCulture tube , Myriad RBM , ref. 782-001282.

Techniques: Gene Expression, Derivative Assay, MANN-WHITNEY

Journal: Cell Reports Medicine

Article Title: Enhanced TLR3 responsiveness in hepatitis C virus resistant women from the Irish anti-D cohort

doi: 10.1016/j.xcrm.2022.100804

Figure Lengend Snippet: Inflammatory cytokine production is increased in polyIC stimulated blood from ESN women Secreted cytokines from polyIC stimulated whole blood were quantified using Luminex and Simoa. (A) Spider plot of polyIC-induced cytokines differentially expressed between ESN and SP donors (∗p < 0.05, ∗∗p < 0.01). (B and C) IFNβ (B) and IFNα (C) protein levels measured using Simoa digital ELISA in supernatants from ESN and SP women after polyIC stimulation. Data are presented on a log 10 scale with median line reported as a solid line. (D and E) Correlation between IFNβ (D) and IFNα (E) protein levels and the polyIC-induced IFN-I score. For Luminex and IFNα Simoa, n = 10 ESN, 20 SP; for everything else, n = 18 ESN, 36 SP. Comparisons between ESNs and SPs were made using Mann-Whitney U tests (∗p < 0.05, ∗∗p < 0.01).

Article Snippet: PolyIC TruCulture tube , Myriad RBM , ref. 782-001282.

Techniques: Luminex, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Cell Reports Medicine

Article Title: Enhanced TLR3 responsiveness in hepatitis C virus resistant women from the Irish anti-D cohort

doi: 10.1016/j.xcrm.2022.100804

Figure Lengend Snippet:

Article Snippet: PolyIC TruCulture tube , Myriad RBM , ref. 782-001282.

Techniques: Recombinant, Luminex, Genotyping Assay, Software