Journal: Biomedicines

Article Title: The Identification of Prohibitin in the Rat Heart Mitochondria in Heart Failure

doi: 10.3390/biomedicines9121793

Figure Lengend Snippet: Effects of the administration of AX on the content of PHB, ANT2, VDAC1, and VDAC2 in rat heart mitochondria in heart failure. Upper part—immunostaining with PHB, ANT2, VDAC1, VDAC2, and Tom20 antibodies; Low part—quantification of immunostaining by computer-assisted densitometry presented as a ratio of proteins to Tom20. Bar graphs represent the levels of appropriate proteins; the data are presented as the means ±SD of three independent experiments. * p < 0.05 significant difference in the protein level in comparison with the control (group 1). # p < 0.05 compared to RHM isolated from ISO-injected rats (group 3). The statistical significance of the differences between the pairs of mean values was evaluated using the Student-Newman-Keul test.

Article Snippet: The polyclonal Tom20 (Santa-Cruz, Dallas, TX, USA) and monoclonal GAPDH (Cell Signaling, Danvers, MA, USA) antibodies were used as a loading control.

Techniques: Immunostaining, Comparison, Control, Isolation, Injection

Journal: Biomedicines

Article Title: The Identification of Prohibitin in the Rat Heart Mitochondria in Heart Failure

doi: 10.3390/biomedicines9121793

Figure Lengend Snippet: Effects of the administration of AX on the content of ATPB, ATP5F1, ATP5G, pGSK3β and GSK3β in rat heart mitochondria in heart failure. ( a )—immunostaining with ATPB, ATP5F1, ATP5G, and Tom20 antibodies (upper part); low part—quantification of immunostaining by computer-assisted densitometry presented as a ratio of proteins to Tom20; ( b )—immunostaining with pGSK3β, GSK3β, and Tom20 antibodies (upper part); low part—quantification of immunostaining by computer-assisted densitometry presented as a ratio of proteins to Tom20. Bar graphs represent the levels of appropriate proteins; the data are presented as the means ± SD of three independent experiments. * p < 0.05 significant difference in the protein level in comparison with the control (group 1). # p < 0.05 compared to RHM isolated from ISO-injected rats (group 3). The statistical significance of the differences between the pairs of mean values was evaluated using the Student-Newman-Keul test.

Article Snippet: The polyclonal Tom20 (Santa-Cruz, Dallas, TX, USA) and monoclonal GAPDH (Cell Signaling, Danvers, MA, USA) antibodies were used as a loading control.

Techniques: Immunostaining, Comparison, Control, Isolation, Injection

Journal: Antioxidants

Article Title: The Effect of Astaxanthin on Mitochondrial Dynamics in Rat Heart Mitochondria under ISO-Induced Injury

doi: 10.3390/antiox12061247

Figure Lengend Snippet: The influence of AX and ISO change of DRP1, Mfn2, and OPA1 in RHM. ( a ) upper part—Western blot with antibody to DRP1. Tom20 was used for normalization of proteins. Lower part—quantitative characteristic presented as the ratio of DRP1 to Tom20; ( b ) upper part—Western blot with antibody to Mfn2, lower part—quantitative characteristic presented as the ratio of Mfn2 to Tom20; ( c ) upper part—Western blot with antibody to OPA1, lower part—quantitative characteristic presented as the ratio of OPA1 to Tom20. The data are presented as the means ± SDs of four independent experiments. * p < 0.05 significant values compared with control (group 1); # p < 0.05 significant values relative ISO to (group 3).

Article Snippet: The polyclonal anti-Tom20 antibodies (Cell Signaling, Danvers, MA, USA) at dilution 1:1000 were used to normalize the protein.

Techniques: Western Blot

Journal: Antioxidants

Article Title: The Effect of Astaxanthin on Mitochondrial Dynamics in Rat Heart Mitochondria under ISO-Induced Injury

doi: 10.3390/antiox12061247

Figure Lengend Snippet: The influence of AX and ISO change of PHB2 and PINK1 in RHM. ( a ) upper part—Western blot with antibody to PHB2. Tom20 was used for normalization of protein. Lower part—quantitative characteristic presented as the ratio of PHB2 to Tom20; ( b ) upper part—Western blot with antibody to PINK1, lower part—quantitative characteristic presented as the ratio of PINK1 to Tom20. The data are presented as the means ± SDs of four independent experiments. * p < 0.05 significant values compared with control (group 1); # p < 0.05 significant values relative to ISO (group 3).

Article Snippet: The polyclonal anti-Tom20 antibodies (Cell Signaling, Danvers, MA, USA) at dilution 1:1000 were used to normalize the protein.

Techniques: Western Blot

Journal: Cell calcium

Article Title: Paclitaxel-induced increase in mitochondrial volume mediates dysregulation of intracellular Ca 2+ in putative nociceptive glabrous skin neurons from the rat

doi: 10.1016/j.ceca.2017.01.005

Figure Lengend Snippet: (A) Live-cell confocal microscopy composite (top; green = mitotracker green, orange-red = DiI, blue = IB4) and mitotracker green (bottom) images of neurons from naïve, vehicle, and paclitaxel treated rats innervating the glabrous skin of the hindpaw, and dorsal hindpaw and inner thigh skin from paclitaxel treated rats. (B) Pooled mitotracker green intensity data from neurons from naïve (white bars), vehicle treated (gray bars), and paclitaxel treated (black bars) rats innervating the glabrous hindpaw, dorsal hindpaw, and inner thigh skin. There was a significant (p < 0.05, two-way ANOVA) interaction between paclitaxel treatment and target of innervation. (C) As in Fig. 2, to determine whether the change in mitotracker staining was significant between groups, data were analyzed as a percent change from control. In this case, because there were no detectable differences between neurons from naïve and vehicle treated rats, data from these two groups of neurons were pooled for each target of innervation. The difference between groups defined by target of innervation was significant (p < 0.01, one-way ANOVA). Composite (top; green = Tom20, orange-red = DiI, DAPI = blue, light gray = IB4) and Tom20 (bottom) images (D) and group mean data (E) from glabrous skin neurons from vehicle and paclitaxel treated rats. Scale bars are 20 μm for all panels in (A) and 25 μm for both panels in (D). The number of neurons in each group is in parenthesis in each bar. ** is p < 0.01. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Rabbit polyclonal anti-Tom20 antibody (Cat # sc-11415, Santa Cruz, Dallas, TX, USA: Antibody Registry: AB 2207533) was diluted in blocking buffer (0.2% Triton X-100, 3% normal donkey serum in phosphate buffer solution (PBS)) to 1:2000.

Techniques: Confocal Microscopy, Staining, Control

Journal: Cell calcium

Article Title: Paclitaxel-induced increase in mitochondrial volume mediates dysregulation of intracellular Ca 2+ in putative nociceptive glabrous skin neurons from the rat

doi: 10.1016/j.ceca.2017.01.005

Figure Lengend Snippet: (A) Confocal images of Tom20 staining (top is mid-section on the z-axis, bottom is mid-section on the y-axis) in neurons from vehicle (left) and paclitaxel (right) treated rats. Images were post-processed with 20 deconvolution iterations, to enable resolution of individual mitochondria. Predicted mitochondria as determined with deconvolution are outlined in red. Pooled data from 8 neurons in each group analyzed as in A, for percent cytoplasmic volume comprised of mitochondria (B) and number of distinct mitochondria units per neuron (C). * is p < 0.05 and ** is p < 0.01.

Article Snippet: Rabbit polyclonal anti-Tom20 antibody (Cat # sc-11415, Santa Cruz, Dallas, TX, USA: Antibody Registry: AB 2207533) was diluted in blocking buffer (0.2% Triton X-100, 3% normal donkey serum in phosphate buffer solution (PBS)) to 1:2000.

Techniques: Staining

Journal: Cell calcium

Article Title: Paclitaxel-induced increase in mitochondrial volume mediates dysregulation of intracellular Ca 2+ in putative nociceptive glabrous skin neurons from the rat

doi: 10.1016/j.ceca.2017.01.005

Figure Lengend Snippet: Confocal microscopy composite images of Tom20 (green) and DiI (red) staining in DRG slices from vehicle (left) and paclitaxel (right) treated rats. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Rabbit polyclonal anti-Tom20 antibody (Cat # sc-11415, Santa Cruz, Dallas, TX, USA: Antibody Registry: AB 2207533) was diluted in blocking buffer (0.2% Triton X-100, 3% normal donkey serum in phosphate buffer solution (PBS)) to 1:2000.

Techniques: Confocal Microscopy, Staining