Journal: Molecular Cell

Article Title: MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis

doi: 10.1016/j.molcel.2019.02.014

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-H2A (WB) , Merck , 07-146; RRID: AB_11212920.

Techniques: Recombinant, Protease Inhibitor, Plasmid Preparation, Staining, Mutagenesis, Mass Spectrometry, Software, Sequencing

Journal: Oncology Reports

Article Title: Effects of low-intensity ultrasound combined with low-dose carboplatin in an orthotopic hamster model of tongue cancer: A preclinical study

doi: 10.3892/or.2018.6262

Figure Lengend Snippet: LIUS and CBP induce DNA damage and cell cycle arrest. (A) γ-H2AX, MGMT, Gadd45α, Chk1, cyclin D1, and cyclin B1 expression was evaluated by immunohistochemical staining. Representative data from three independent experiments are shown. Scale bar, 50 µm. (B) The expression levels of MGMT, Gadd45α, Chk1, cyclin D1, cyclin B1, caspase-3, and cleaved caspase-3 protein were assessed by western blotting; β-actin was used as the internal control. Data are presented as the mean ± SD (n=3). Statistical significance was determined by one-way ANOVA followed by Dunnett's test. *P<0.05 vs. the CB20 group. LIUS, low-intensity ultrasound; CBP, carboplatin; MGMT, O 6 -methylguanine DNA methyltransferase.

Article Snippet: Rabbit polyclonal anti-Bak (cat. no. PB0506; IHC, 1:100; WB, 1:500), rabbit polyclonal anti-caspase-3 (cat. no. BA3968; IHC, 1:100; WB, 1:300) and rabbit polyclonal anti-cyclin D1 (cat. no. BA0770; IHC, 1:100; WB, 1:300) were purchased from Wuhan Boster Bioengineering Co., Ltd. (Wuhan, China), rabbit polyclonal anti-MGMT (cat. no. BS1002R; IHC, 1:500; WB, 1:1,000) was purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China), Mouse monoclonal anti-γ-H2AX (cat. no. ab26350; IHC, 1:100; WB, 1:1,000) was provided by Abcam (Cambridge, UK) and rabbit polyclonal anti-Gadd45α (cat. no. E2A6622; IHC, 1:100; WB, 1:300) was purchased from EnoGene (Nanjing, China).

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot

Journal: Oncology Reports

Article Title: Effects of low-intensity ultrasound combined with low-dose carboplatin in an orthotopic hamster model of tongue cancer: A preclinical study

doi: 10.3892/or.2018.6262

Figure Lengend Snippet: The expression of MGMT, GADD45α, and Chk1 in (A-C) non-carcinoma tissues and (D-F) OTSCC tissues (×400, magnification). MGMT, O 6 -methylguanine DNA methyltransferase; OTSCC, oral tongue squamous cell carcinoma.

Article Snippet: Rabbit polyclonal anti-Bak (cat. no. PB0506; IHC, 1:100; WB, 1:500), rabbit polyclonal anti-caspase-3 (cat. no. BA3968; IHC, 1:100; WB, 1:300) and rabbit polyclonal anti-cyclin D1 (cat. no. BA0770; IHC, 1:100; WB, 1:300) were purchased from Wuhan Boster Bioengineering Co., Ltd. (Wuhan, China), rabbit polyclonal anti-MGMT (cat. no. BS1002R; IHC, 1:500; WB, 1:1,000) was purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China), Mouse monoclonal anti-γ-H2AX (cat. no. ab26350; IHC, 1:100; WB, 1:1,000) was provided by Abcam (Cambridge, UK) and rabbit polyclonal anti-Gadd45α (cat. no. E2A6622; IHC, 1:100; WB, 1:300) was purchased from EnoGene (Nanjing, China).

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: Pyrroloquinoline quinone inhibits PCSK9-NLRP3 mediated pyroptosis of Leydig cells in obese mice

doi: 10.1038/s41419-023-06162-8

Figure Lengend Snippet: Key resources.

Article Snippet: Rabbit polyclonal anti-CYP11a1 (IHC: 1:150, WB: 1:1000) , Abmart , Cat# PU986898; N/A.

Techniques: Recombinant, Western Blot, Stripping Membranes, Staining, Binding Assay, Saline, Cholesterol Assay, Bicinchoninic Acid Protein Assay, LDH Cytotoxicity Assay, CCK-8 Assay, Transfection, Software, Membrane

Journal: Cell reports

Article Title: Pro-inflammatory β cell small extracellular vesicles induce β cell failure through activation of the CXCL10/CXCR3 axis in diabetes

doi: 10.1016/j.celrep.2021.109613

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Alix Antibody, rabbit polyclonal (WB 1:1000) , System Biosciences , Cat# EXOAB-ALIX-1; RRID: AB_2892999.

Techniques: Recombinant, Plasmid Preparation, SYBR Green Assay, Lysis, Labeling, In Situ, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Cell Isolation, Chemotaxis Assay, Bicinchoninic Acid Protein Assay, Software

Journal: eLife

Article Title: Brucella activates the host RIDD pathway to subvert BLOS1-directed immune defense

doi: 10.7554/eLife.73625

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit Polyclonal IHCPlus ARL8B Antibody aa72-121 Polyclonal IHC, WB LS-B5831 , LifeSpan Biosciences , Cat# LS-B5831-100 , 5 µg/ml dilution.

Techniques: Derivative Assay, Mutagenesis, Plasmid Preparation, Cell Culture, Transfection, Construct, Expressing, Recombinant, Sequencing, Synthesized, Phagocytosis Assay, Immunoprecipitation, TA Cloning, Subcloning, SYBR Green Assay, Blocking Assay, Western Blot, Stripping Membranes, Molecular Weight, Staining, Software, Functional Assay