polyclonal cdk4 antibody Search Results


cdk4  (Bioss)
93
Bioss cdk4
The effect of circTIAM1 on adipocyte proliferation. ( A ) circTIAM1 and TIAM1 expression after transfection of pCD2.1-circTIAM1 into adipocytes. ( B ) circTIAM1 and TIAM1 expression after transfection of si-circTIAM1 into adipocytes. ( C ) qRT-PCR analysis of cyclin D3, <t>CDK4,</t> cyclin B, and PCNA mRNA expression after pCD2.1-circTIAM1 transfection into adipocytes. ( D ) qRT-PCR analysis of cyclin D3, CDK4, cyclin B, and PCNA mRNA expression after si-circTIAM1 transfection into adipocytes. ( E ) Western blot analysis of cyclin D3 and <t>CDK4</t> <t>protein</t> expression after circTIAM1 overexpression and interference. ( F , G ) Protein grayscale value analysis. ( H ) EdU analysis of adipocyte proliferation. ( I , J ) CCK-8 analysis of adipocyte proliferation. ** p < 0.01, *** p < 0.001.
Cdk4, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdk4/product/Bioss
Average 93 stars, based on 1 article reviews
cdk4 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
cdk4/6 inhibitor  (Mitotix Inc)
90
Mitotix Inc cdk4/6 inhibitor
The effect of circTIAM1 on adipocyte proliferation. ( A ) circTIAM1 and TIAM1 expression after transfection of pCD2.1-circTIAM1 into adipocytes. ( B ) circTIAM1 and TIAM1 expression after transfection of si-circTIAM1 into adipocytes. ( C ) qRT-PCR analysis of cyclin D3, <t>CDK4,</t> cyclin B, and PCNA mRNA expression after pCD2.1-circTIAM1 transfection into adipocytes. ( D ) qRT-PCR analysis of cyclin D3, CDK4, cyclin B, and PCNA mRNA expression after si-circTIAM1 transfection into adipocytes. ( E ) Western blot analysis of cyclin D3 and <t>CDK4</t> <t>protein</t> expression after circTIAM1 overexpression and interference. ( F , G ) Protein grayscale value analysis. ( H ) EdU analysis of adipocyte proliferation. ( I , J ) CCK-8 analysis of adipocyte proliferation. ** p < 0.01, *** p < 0.001.
Cdk4/6 Inhibitor, supplied by Mitotix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdk4/6 inhibitor/product/Mitotix Inc
Average 90 stars, based on 1 article reviews
cdk4/6 inhibitor - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
anti-cdk4 antibody picoband  (Boster Bio)
92
Boster Bio anti-cdk4 antibody picoband
The effect of circTIAM1 on adipocyte proliferation. ( A ) circTIAM1 and TIAM1 expression after transfection of pCD2.1-circTIAM1 into adipocytes. ( B ) circTIAM1 and TIAM1 expression after transfection of si-circTIAM1 into adipocytes. ( C ) qRT-PCR analysis of cyclin D3, <t>CDK4,</t> cyclin B, and PCNA mRNA expression after pCD2.1-circTIAM1 transfection into adipocytes. ( D ) qRT-PCR analysis of cyclin D3, CDK4, cyclin B, and PCNA mRNA expression after si-circTIAM1 transfection into adipocytes. ( E ) Western blot analysis of cyclin D3 and <t>CDK4</t> <t>protein</t> expression after circTIAM1 overexpression and interference. ( F , G ) Protein grayscale value analysis. ( H ) EdU analysis of adipocyte proliferation. ( I , J ) CCK-8 analysis of adipocyte proliferation. ** p < 0.01, *** p < 0.001.
Anti Cdk4 Antibody Picoband, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-cdk4 antibody picoband/product/Boster Bio
Average 92 stars, based on 1 article reviews
anti-cdk4 antibody picoband - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier

Image Search Results


The effect of circTIAM1 on adipocyte proliferation. ( A ) circTIAM1 and TIAM1 expression after transfection of pCD2.1-circTIAM1 into adipocytes. ( B ) circTIAM1 and TIAM1 expression after transfection of si-circTIAM1 into adipocytes. ( C ) qRT-PCR analysis of cyclin D3, CDK4, cyclin B, and PCNA mRNA expression after pCD2.1-circTIAM1 transfection into adipocytes. ( D ) qRT-PCR analysis of cyclin D3, CDK4, cyclin B, and PCNA mRNA expression after si-circTIAM1 transfection into adipocytes. ( E ) Western blot analysis of cyclin D3 and CDK4 protein expression after circTIAM1 overexpression and interference. ( F , G ) Protein grayscale value analysis. ( H ) EdU analysis of adipocyte proliferation. ( I , J ) CCK-8 analysis of adipocyte proliferation. ** p < 0.01, *** p < 0.001.

Journal: International Journal of Molecular Sciences

Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway

doi: 10.3390/ijms25094588

Figure Lengend Snippet: The effect of circTIAM1 on adipocyte proliferation. ( A ) circTIAM1 and TIAM1 expression after transfection of pCD2.1-circTIAM1 into adipocytes. ( B ) circTIAM1 and TIAM1 expression after transfection of si-circTIAM1 into adipocytes. ( C ) qRT-PCR analysis of cyclin D3, CDK4, cyclin B, and PCNA mRNA expression after pCD2.1-circTIAM1 transfection into adipocytes. ( D ) qRT-PCR analysis of cyclin D3, CDK4, cyclin B, and PCNA mRNA expression after si-circTIAM1 transfection into adipocytes. ( E ) Western blot analysis of cyclin D3 and CDK4 protein expression after circTIAM1 overexpression and interference. ( F , G ) Protein grayscale value analysis. ( H ) EdU analysis of adipocyte proliferation. ( I , J ) CCK-8 analysis of adipocyte proliferation. ** p < 0.01, *** p < 0.001.

Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China), PLCB1 (1:1000, 26551-1-AP, proteintech, Wuhan, China), and β-actin (1:1000, bs-0061R, Bioss, Beijing, China).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot, Over Expression, CCK-8 Assay

The effect of miR-485-3p on Adipocyte Proliferation. ( A ) miR-485-3p expression levels after transfection of miR-485-3p mimic and miR-485-3p inhibitor into adipocytes. ( B ) qRT-PCR analysis of Cyclin D3, CDK4, Cyclin B, and PCNA mRNA expression after overexpression and knockdown of miR-485-3p. ( C , D ) Western blot analysis of Cyclin D3 and CDK4 protein expression after miR-485-3p overexpression and knockdown. ( E ) EdU assay for adipocyte proliferation. ( F , G ) CCK-8 assay illustrating adipocyte proliferation. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: International Journal of Molecular Sciences

Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway

doi: 10.3390/ijms25094588

Figure Lengend Snippet: The effect of miR-485-3p on Adipocyte Proliferation. ( A ) miR-485-3p expression levels after transfection of miR-485-3p mimic and miR-485-3p inhibitor into adipocytes. ( B ) qRT-PCR analysis of Cyclin D3, CDK4, Cyclin B, and PCNA mRNA expression after overexpression and knockdown of miR-485-3p. ( C , D ) Western blot analysis of Cyclin D3 and CDK4 protein expression after miR-485-3p overexpression and knockdown. ( E ) EdU assay for adipocyte proliferation. ( F , G ) CCK-8 assay illustrating adipocyte proliferation. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China), PLCB1 (1:1000, 26551-1-AP, proteintech, Wuhan, China), and β-actin (1:1000, bs-0061R, Bioss, Beijing, China).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Over Expression, Knockdown, Western Blot, EdU Assay, CCK-8 Assay

circTIAM1 as a ceRNA for miR-485-3p. ( A ) Predicted binding sites between circTIAM1 and miR-485-3p. ( B ) Binding and mutation sites of circTIAM1 with miR-485-3p. ( C ) Luciferase activity of wildtype and mutant circTIAM1 vectors cotransfected with miR-485-3p mimic or miR-485-3p NC into 293 T cells after 48 h. ( D ) circTIAM1, miR-485-3p, and PLCB1 expression patterns during adipocyte differentiation. ( E ) qRTPCR analysis of mRNA of cyclin D3, CDK4, cyclin B, and PCNA expression levels. ( F ) qRTPCR analysis of mRNA of Adiponectin, C/EBPα, FABP4, and PPARγ expression levels. * p < 0.05, ** p < 0.01.

Journal: International Journal of Molecular Sciences

Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway

doi: 10.3390/ijms25094588

Figure Lengend Snippet: circTIAM1 as a ceRNA for miR-485-3p. ( A ) Predicted binding sites between circTIAM1 and miR-485-3p. ( B ) Binding and mutation sites of circTIAM1 with miR-485-3p. ( C ) Luciferase activity of wildtype and mutant circTIAM1 vectors cotransfected with miR-485-3p mimic or miR-485-3p NC into 293 T cells after 48 h. ( D ) circTIAM1, miR-485-3p, and PLCB1 expression patterns during adipocyte differentiation. ( E ) qRTPCR analysis of mRNA of cyclin D3, CDK4, cyclin B, and PCNA expression levels. ( F ) qRTPCR analysis of mRNA of Adiponectin, C/EBPα, FABP4, and PPARγ expression levels. * p < 0.05, ** p < 0.01.

Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China), PLCB1 (1:1000, 26551-1-AP, proteintech, Wuhan, China), and β-actin (1:1000, bs-0061R, Bioss, Beijing, China).

Techniques: Binding Assay, Mutagenesis, Luciferase, Activity Assay, Expressing

qPCR primers for mRNA application.

Journal: International Journal of Molecular Sciences

Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway

doi: 10.3390/ijms25094588

Figure Lengend Snippet: qPCR primers for mRNA application.

Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China), PLCB1 (1:1000, 26551-1-AP, proteintech, Wuhan, China), and β-actin (1:1000, bs-0061R, Bioss, Beijing, China).

Techniques: