Journal: EMBO Reports

Article Title: An hepatitis B and D virus infection model using human pluripotent stem cell-derived hepatocytes

doi: 10.1038/s44319-024-00236-0

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit anti-FoxA2 (1:400) was purchased from Cell Signaling, mouse anti-AFP (1:1000) from Sigma-Aldrich, mouse anti-ALB (1:1000) from Cedarlane, mouse anti-CD63 from Santa Cruz Biotechnology (1:400), rabbit anti-ADAR1 from Cell Signaling (1:1000), rabbit anti-LDLR from Abcam (1:200), rabbit anti-LAMP1 (1:200) and rabbit anti-EGFR (1:100) from Cell Signaling and rabbit anti-SCARB1 from Novus Biologicals (1:500).

Techniques: Recombinant, Immunofluorescence, Western Blot, Sequencing, Membrane, Knock-Out, Transfection, Reverse Transcription, SYBR Green Assay, Protease Inhibitor, Diagnostic Assay, Software, Microscopy, Imaging

Journal: Journal of Neuroimmunology

Article Title: PKR mediated regulation of inflammation and IL-10 during viral encephalomyelitis

doi: 10.1016/j.jneuroim.2014.02.012

Figure Lengend Snippet: PKR deficiency results in increased perivascular accumulation of CD4 T cells. (A) Localization of CD4 T cells was assessed at days 7 and 10 p.i. by dual staining of brain sections for laminin (green) and CD4 (red) and analyzed by confocal microscopy. Bar graphs show average of CD4 T cells in perivascular space and in brain parenchyma of 6–9 fields/brain with n = 6 mice/group/time-point ± SEM. (B) Staining of brain sections at day 7 p.i. for PKR-p (green), CD4 (red) and nuclei (DAPI, blue) was analyzed by florescent microscopy. Images for both panels are representative of 6–9 fields/brain with n = 6 mice/group/time-point. *p ≥ .05.

Article Snippet: The distribution of CD4 T cells in brain parenchyma and perivascular space was determined by staining brain sections with rabbit anti-mouse laminin Ab (Cedarlane Laboratories, Ontario, Canada) and rat anti-mouse CD4 mAb (BD Pharmingen).

Techniques: Staining, Confocal Microscopy, Microscopy