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Image Search Results

Journal: Neural Regeneration Research
Article Title: Saponins from Panax japonicus attenuate age-related neuroinflammation via regulation of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways
doi: 10.4103/1673-5374.219047
Figure Lengend Snippet: Effect of SPJ on levels of p-ERK, p-JNK, and p-p38 MAPK in the cortex and hippocampus of aging rats. (A) Representative p-ERK and ERK protein bands tested by western blot assay; (B) data quantification of p-ERK; (C) representative bands of p-JNK and JNK proteins tested by western blot assay; (D) data quantification of p-JNK; (E) representative bands of p-p38 and p38 proteins tested by western blot assay; (F) data quantification of p-p38. * P
Article Snippet: The primary antibodies used were TNF-α (goat polyclonal antibody, diluted 1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA), IL-1β (rabbit polyclonal antibody, diluted 1:500; Santa Cruz Biotechnology), inducible nitric oxide synthase (iNOS) (mouse monoclonal antibody, diluted 1:500; Santa Cruz Biotechnology), cyclooxygenase-2 (COX-2) (rabbit monoclonal antibody, diluted 1:1,000; Cell Signaling, Danvers, MA, USA), NF-κB p65 (mouse monoclonal antibody, diluted 1:1,000; Cell Signaling), lamin A (rabbit polyclonal antibody, diluted 1:1,000; Santa Cruz Biotechnology), phospho-NF-κB (p-NF-κB) p65 (rabbit polyclonal antibody, diluted 1:1,000; Cell Signaling), phospho-inhibitor of kappa B (p-IκBα) (mouse monoclonal antibody, diluted 1:1,000; Cell Signaling), IκBα (rabbit monoclonal antibody, diluted 1:1,000; Cell Signaling), ERK (rabbit polyclonal antibody, diluted 1:500, Santa Cruz Biotechnology), phospho-ERK (p-ERK) (mouse monoclonal antibody, diluted 1:500; Santa Cruz Biotechnology), c-Jun N-terminal kinase (JNK) (rabbit polyclonal antibody, diluted 1:1,000; Cell Signaling), phospho-c-Jun N-terminal kinase (p-JNK) (rabbit monoclonal antibody, diluted 1:1,000; Cell Signaling),
Techniques: Western Blot