polyclonal anti rabbit cdc2 p34 Search Results


86
Santa Cruz Biotechnology rabbit anti human cdc2 p34 cdk1
USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, <t>CDK1,</t> and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.
Rabbit Anti Human Cdc2 P34 Cdk1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti human cdc2 p34 cdk1/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti human cdc2 p34 cdk1 - by Bioz Stars, 2024-11
86/100 stars
  Buy from Supplier

86
Santa Cruz Biotechnology rabbit anti cdc2 p34
USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, <t>CDK1,</t> and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.
Rabbit Anti Cdc2 P34, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti cdc2 p34/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti cdc2 p34 - by Bioz Stars, 2024-11
86/100 stars
  Buy from Supplier

86
Santa Cruz Biotechnology polyclonal anti rabbit cdc2 p34
USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, <t>CDK1,</t> and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.
Polyclonal Anti Rabbit Cdc2 P34, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal anti rabbit cdc2 p34/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
polyclonal anti rabbit cdc2 p34 - by Bioz Stars, 2024-11
86/100 stars
  Buy from Supplier

86
Kinetek Pharmaceuticals Inc rabbit polyclonal anti p34 cdc2
USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, <t>CDK1,</t> and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.
Rabbit Polyclonal Anti P34 Cdc2, supplied by Kinetek Pharmaceuticals Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti p34 cdc2/product/Kinetek Pharmaceuticals Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal anti p34 cdc2 - by Bioz Stars, 2024-11
86/100 stars
  Buy from Supplier

86
Abcam rabbit polyclonal anti p34 cdc2 antibody
USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, <t>CDK1,</t> and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.
Rabbit Polyclonal Anti P34 Cdc2 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti p34 cdc2 antibody/product/Abcam
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal anti p34 cdc2 antibody - by Bioz Stars, 2024-11
86/100 stars
  Buy from Supplier

Image Search Results


USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, CDK1, and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.

Journal: Journal of Virology

Article Title: USP18 (UBP43) Abrogates p21-Mediated Inhibition of HIV-1

doi: 10.1128/JVI.00592-18

Figure Lengend Snippet: USP18 interacts with SKP2 and SAMHD1 and complexes with cyclin A2, CDK1, and CDK2. (A) Immunoprecipitation of HA-USP18, coexpressed with FLAG-SAMHD1 (lane 2), MYC-SKP2 (lane 3) or all three expression plasmids in a ratio of 1:1:1 (lane 4) using anti-HA affinity matrix beads. In parallel, FLAG-SAMHD1 was coexpressed with MYC-SKP2 and immunoprecipitated with anti-FLAG affinity matrix beads (lanes 7). Single and double transfections were supplemented with pcDNA3.1(+). Cell lysates were immunoblotted for USP18, SKP2, and SAMHD1, using their respective epitopped tags. (B) Immunoprecititation of FLAG-SAMHD1 coexpressed with pLOC-USP18 or without pLOC-USP18 and replaced by pcDNA3.1+. Proteins of HEK293T cells were immunoprecipitated with anti-FLAG affinity matrix beads and immunoblotted with FLAG and USP18 and endogenous expression of cyclin A2 and CDK1, cyclin A2 (B), and CDK2 (C). Each panel is representative of at least three independent experiments.

Article Snippet: In other experiments, endogenous cyclin A2, CDK1, and CDK2 were detected using rabbit anti-human cyclin A2 (18202-1-AP; Proteintech; 1:500 dilution), rabbit anti-human Cdc2 p34/CDK1 (H-297, sc-747; Santa Cruz; 1:500), and rabbit anti-human CDK2 (78B2, 2546; Cell Signaling; 1:500), respectively, after immunoprecipitation of overexpressed SAMHD1-FLAG cotransfected with USP18.

Techniques: Immunoprecipitation, Expressing, Transfection