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Image Search Results

Journal: bioRxiv
Article Title: TFIIB-related factor 1 is a nucleolar protein that promotes RNA polymerase I-directed transcription and tumour cell growth
doi: 10.1101/2022.02.20.481212
Figure Lengend Snippet: (A) BRF1 and TIF-1A were co-localized in the nucleoli of HeLa cells. IF staining was performed using the BRF1 and TIF-1A antibodies. The scale bar in each image represents 2.5 μm. ( B ) BRF1 and RPA40 were co-localized in the nucleoli of HeLa cells. IF staining was performed using BRF1 antibody and RPA40 antibody. The scale bar in each image represents 2.5 μm. ( C ) The co-localization analysis between BRF1 and TIF-1A using the nucleolar particles purified from HeLa cells. The scale bar in each image represents 2.5 μm. ( D ) The co-localization analysis between BRF1 and RPA43 using the nucleoli particles purified from HeLa cells. The scale bar in each image represents 5 μm. ( E ) Components of the Pol I transcription machinery could be precipitated by the BRF1 antibody. HeLa nuclear extract was prepared and used for BRF1 IP assays. BRF1-binding proteins in the IP samples were detected by Western blot using the antibodies as indicated in the panel. ( F , G ) BRF1 could be precipitated by the antibodies against Pol I-related proteins. IP assays were performed with the antibodies against TBP, TIF-1A, TAF1A, UBF, and RPA43, respectively. BRF1 ( F ) and proteins as indicated ( G ) were detected by Western blot. Figure 6-figure supplement 1. The co-localization analysis between BRF1 and Pol III transcription factors in HeLa cells. Figure 6-figure supplement 2. BRF1 binds to TFIIIC subunit GTF3C2 and SL1 subunit TAF1B. Figure 6-source data, Figure 6-figure supplement 2-source data
Article Snippet: IP assays were performed using normal IgG, BRF1 antibody and the antibodies against UBF (ab244287, Abcam), TIF-1A (ab251933, Abcam), TBP (ab28175, abcam), TAF1A (SC-393600, Santa Cruz Biotech), GTF3C2 (SC-81406, Santa Cruz Biotech),
Techniques: Staining, Purification, Binding Assay, Western Blot

Journal: bioRxiv
Article Title: TFIIB-related factor 1 is a nucleolar protein that promotes RNA polymerase I-directed transcription and tumour cell growth
doi: 10.1101/2022.02.20.481212
Figure Lengend Snippet: (A) BRF1 IP results showing BRF1 association with GTF3C2 and TAF1B. IP assays were performed using BRF1 antibody and HeLa nuclear extract. BRF1-bound protein was detected by Western blot using the antibodies as indicated. ( B ) GTF3C2 IP result showing GT3C2 association with BRF1. IP assays were performed using GTF3C2 antibody and HeLa nuclear extract. GTF3C2-bound protein was detected by Western blot using the antibodies as indicated. ( C ) TAF1B IP result showing TAF1B association with BRF1. IP assays were performed using TAF1B antibody and HeLa nuclear extract. TAF1B-bound protein was detected by Western blot using the antibodies as indicated.
Article Snippet: IP assays were performed using normal IgG, BRF1 antibody and the antibodies against UBF (ab244287, Abcam), TIF-1A (ab251933, Abcam), TBP (ab28175, abcam), TAF1A (SC-393600, Santa Cruz Biotech), GTF3C2 (SC-81406, Santa Cruz Biotech),
Techniques: Western Blot