Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Dissolvable Polyacrylamide Beads for High-Throughput Droplet DNA Barcoding.

doi: 10.1002/advs.201903463

Figure Lengend Snippet: Figure 1. A) Schematic diagram of the dissolvable polyacrylamide beads synthesis and dissolution mechanism. Acrylamide monomers and N,N- bis(acryloyl)cystamine crosslinker can react through CC double bonds to form a polymer bead in the presence of ammonium persulfate (APS) and tetramethylethylenediamine (TEMED) at 70 °C overnight; the formed polyacrylamide bead can be dissolved when disulfide bonds are cleaved by DTT. B) Schematic diagram the dissolvable beads used for droplet-based single cell analysis.

Article Snippet: For these applications, the beads are mostly made of biocompatible polymers, for example, polyacrylamide beads in inDrop,[2] hydroxylated methacrylic polymer beads used in Drop-seq,[3] and polyacrylamide beads used in 10X Genomics.

Techniques: Dissolution, Polymer, Single-cell Analysis

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Dissolvable Polyacrylamide Beads for High-Throughput Droplet DNA Barcoding.

doi: 10.1002/advs.201903463

Figure Lengend Snippet: Figure 2. Fabrication and characterization of dissolvable polyacrylamide beads. A) Dissolution time of the beads with different DTT concentrations. B) Microscopic images of dissolving polyacrylamide beads at 0, 60, 120, and 180 s with 1 × 10−3 m DTT. C) Serial fluorescence images of FP-labeled beads upon addition of 1 × 10−3 m DTT at 60, 120, and 180 s. The first image represents the original signal intensity before DTT addition. D) quantita- tive analysis of the fluorescence of eight representative beads versus time shows that the fluorescence of the bead is exponentially decaying. Each line represents a bead.

Article Snippet: For these applications, the beads are mostly made of biocompatible polymers, for example, polyacrylamide beads in inDrop,[2] hydroxylated methacrylic polymer beads used in Drop-seq,[3] and polyacrylamide beads used in 10X Genomics.

Techniques: Dissolution, Fluorescence, Labeling