Journal: STAR Protocols

Article Title: Protocol for identification of proteins from deyolked zebrafish embryos

doi: 10.1016/j.xpro.2025.103728

Figure Lengend Snippet:

Article Snippet: 10% polyacrylamide gel , Bio-Rad , Cat#3450053.

Techniques: Recombinant, Protease Inhibitor, Qubit Protein Assay, Software

Journal: Journal of Biological Chemistry

Article Title: RhoA Prenylation Is Required for Promotion of Cell Growth and Transformation and Cytoskeleton Organization but Not for Induction of Serum Response Element Transcription

doi: 10.1074/jbc.m005264200

Figure Lengend Snippet: FIG. 3. In vitro interaction of GST-RhoGDI with prenylated RhoA. 100 mg of cell lysates of COS-7 cells transiently transfected with pCMV-WT RhoA were incubated with purified GST-RhoGDI-1 fusion protein as described under “Experimental Procedures.” The proteins bound on beads were then denatured in sample buffer and separated by SDS-polyacrylamide gel electrophoresis, followed by Western blot (GST-RhoGDI-1 bound RhoA). 20 mg of cell lysates were analyzed for expression of RhoA (total RhoA) by Western blot with anti-Myc antibodies.

Article Snippet: 30 mg of the cleared lysates were separated on a 12.5% SDS-polyacrylamide gel, blotted to nitrocellulose membranes (Gelman), and incubated with antibodies against RhoA (26-C4, Santa Cruz Biotechnology), Rap1A/Krev-1 (C-065, Santa Cruz Biotechnology), or Ha-Ras (C-20, Santa Cruz Biotechnology), respectively.

Techniques: In Vitro, Transfection, Incubation, Purification, Polyacrylamide Gel Electrophoresis, Western Blot, Expressing