polyacrylamide Search Results


polyacrylamide  (Thermo Fisher)
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Thermo Fisher polyacrylamide
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hpa  (TaKaRa)
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TaKaRa hpa
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sds polyacrylamide gel  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology sds polyacrylamide gel
FIG. 3. In vitro interaction of GST-RhoGDI with prenylated RhoA. 100 mg of cell lysates of COS-7 cells transiently transfected with pCMV-WT RhoA were incubated with purified GST-RhoGDI-1 fusion protein as described under “Experimental Procedures.” The proteins bound on beads were then denatured in sample buffer and separated by <t>SDS-polyacrylamide</t> gel electrophoresis, followed by Western blot (GST-RhoGDI-1 bound RhoA). 20 mg of cell lysates were analyzed for expression of RhoA (total RhoA) by Western blot with anti-Myc antibodies.
Sds Polyacrylamide Gel, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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criterion tbe urea polyacrylamide gel  (Bio-Rad)
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Bio-Rad criterion tbe urea polyacrylamide gel
FIG. 3. In vitro interaction of GST-RhoGDI with prenylated RhoA. 100 mg of cell lysates of COS-7 cells transiently transfected with pCMV-WT RhoA were incubated with purified GST-RhoGDI-1 fusion protein as described under “Experimental Procedures.” The proteins bound on beads were then denatured in sample buffer and separated by <t>SDS-polyacrylamide</t> gel electrophoresis, followed by Western blot (GST-RhoGDI-1 bound RhoA). 20 mg of cell lysates were analyzed for expression of RhoA (total RhoA) by Western blot with anti-Myc antibodies.
Criterion Tbe Urea Polyacrylamide Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tbe urea gel  (Bio-Rad)
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Bio-Rad tbe urea gel
FIG. 3. In vitro interaction of GST-RhoGDI with prenylated RhoA. 100 mg of cell lysates of COS-7 cells transiently transfected with pCMV-WT RhoA were incubated with purified GST-RhoGDI-1 fusion protein as described under “Experimental Procedures.” The proteins bound on beads were then denatured in sample buffer and separated by <t>SDS-polyacrylamide</t> gel electrophoresis, followed by Western blot (GST-RhoGDI-1 bound RhoA). 20 mg of cell lysates were analyzed for expression of RhoA (total RhoA) by Western blot with anti-Myc antibodies.
Tbe Urea Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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criterion precast polyacrylamide tbe gel  (Bio-Rad)
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Bio-Rad criterion precast polyacrylamide tbe gel
FIG. 3. In vitro interaction of GST-RhoGDI with prenylated RhoA. 100 mg of cell lysates of COS-7 cells transiently transfected with pCMV-WT RhoA were incubated with purified GST-RhoGDI-1 fusion protein as described under “Experimental Procedures.” The proteins bound on beads were then denatured in sample buffer and separated by <t>SDS-polyacrylamide</t> gel electrophoresis, followed by Western blot (GST-RhoGDI-1 bound RhoA). 20 mg of cell lysates were analyzed for expression of RhoA (total RhoA) by Western blot with anti-Myc antibodies.
Criterion Precast Polyacrylamide Tbe Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyacrylamide tbe urea gels  (Bio-Rad)
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Bio-Rad polyacrylamide tbe urea gels
FIG. 3. In vitro interaction of GST-RhoGDI with prenylated RhoA. 100 mg of cell lysates of COS-7 cells transiently transfected with pCMV-WT RhoA were incubated with purified GST-RhoGDI-1 fusion protein as described under “Experimental Procedures.” The proteins bound on beads were then denatured in sample buffer and separated by <t>SDS-polyacrylamide</t> gel electrophoresis, followed by Western blot (GST-RhoGDI-1 bound RhoA). 20 mg of cell lysates were analyzed for expression of RhoA (total RhoA) by Western blot with anti-Myc antibodies.
Polyacrylamide Tbe Urea Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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page tbe gel  (Bio-Rad)
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Bio-Rad page tbe gel
FIG. 3. In vitro interaction of GST-RhoGDI with prenylated RhoA. 100 mg of cell lysates of COS-7 cells transiently transfected with pCMV-WT RhoA were incubated with purified GST-RhoGDI-1 fusion protein as described under “Experimental Procedures.” The proteins bound on beads were then denatured in sample buffer and separated by <t>SDS-polyacrylamide</t> gel electrophoresis, followed by Western blot (GST-RhoGDI-1 bound RhoA). 20 mg of cell lysates were analyzed for expression of RhoA (total RhoA) by Western blot with anti-Myc antibodies.
Page Tbe Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gradient tbe page gels  (Bio-Rad)
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Bio-Rad gradient tbe page gels
FIG. 3. In vitro interaction of GST-RhoGDI with prenylated RhoA. 100 mg of cell lysates of COS-7 cells transiently transfected with pCMV-WT RhoA were incubated with purified GST-RhoGDI-1 fusion protein as described under “Experimental Procedures.” The proteins bound on beads were then denatured in sample buffer and separated by <t>SDS-polyacrylamide</t> gel electrophoresis, followed by Western blot (GST-RhoGDI-1 bound RhoA). 20 mg of cell lysates were analyzed for expression of RhoA (total RhoA) by Western blot with anti-Myc antibodies.
Gradient Tbe Page Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyacrylamide tbe gels  (Bio-Rad)
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Bio-Rad polyacrylamide tbe gels
FIG. 3. In vitro interaction of GST-RhoGDI with prenylated RhoA. 100 mg of cell lysates of COS-7 cells transiently transfected with pCMV-WT RhoA were incubated with purified GST-RhoGDI-1 fusion protein as described under “Experimental Procedures.” The proteins bound on beads were then denatured in sample buffer and separated by <t>SDS-polyacrylamide</t> gel electrophoresis, followed by Western blot (GST-RhoGDI-1 bound RhoA). 20 mg of cell lysates were analyzed for expression of RhoA (total RhoA) by Western blot with anti-Myc antibodies.
Polyacrylamide Tbe Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paa  (Aladdin Scientific Corporation)
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Aladdin Scientific Corporation paa
FIG. 3. In vitro interaction of GST-RhoGDI with prenylated RhoA. 100 mg of cell lysates of COS-7 cells transiently transfected with pCMV-WT RhoA were incubated with purified GST-RhoGDI-1 fusion protein as described under “Experimental Procedures.” The proteins bound on beads were then denatured in sample buffer and separated by <t>SDS-polyacrylamide</t> gel electrophoresis, followed by Western blot (GST-RhoGDI-1 bound RhoA). 20 mg of cell lysates were analyzed for expression of RhoA (total RhoA) by Western blot with anti-Myc antibodies.
Paa, supplied by Aladdin Scientific Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyacrylamide substrates  (TargetMol)
91
TargetMol polyacrylamide substrates
FIG. 3. In vitro interaction of GST-RhoGDI with prenylated RhoA. 100 mg of cell lysates of COS-7 cells transiently transfected with pCMV-WT RhoA were incubated with purified GST-RhoGDI-1 fusion protein as described under “Experimental Procedures.” The proteins bound on beads were then denatured in sample buffer and separated by <t>SDS-polyacrylamide</t> gel electrophoresis, followed by Western blot (GST-RhoGDI-1 bound RhoA). 20 mg of cell lysates were analyzed for expression of RhoA (total RhoA) by Western blot with anti-Myc antibodies.
Polyacrylamide Substrates, supplied by TargetMol, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 3. In vitro interaction of GST-RhoGDI with prenylated RhoA. 100 mg of cell lysates of COS-7 cells transiently transfected with pCMV-WT RhoA were incubated with purified GST-RhoGDI-1 fusion protein as described under “Experimental Procedures.” The proteins bound on beads were then denatured in sample buffer and separated by SDS-polyacrylamide gel electrophoresis, followed by Western blot (GST-RhoGDI-1 bound RhoA). 20 mg of cell lysates were analyzed for expression of RhoA (total RhoA) by Western blot with anti-Myc antibodies.

Journal: Journal of Biological Chemistry

Article Title: RhoA Prenylation Is Required for Promotion of Cell Growth and Transformation and Cytoskeleton Organization but Not for Induction of Serum Response Element Transcription

doi: 10.1074/jbc.m005264200

Figure Lengend Snippet: FIG. 3. In vitro interaction of GST-RhoGDI with prenylated RhoA. 100 mg of cell lysates of COS-7 cells transiently transfected with pCMV-WT RhoA were incubated with purified GST-RhoGDI-1 fusion protein as described under “Experimental Procedures.” The proteins bound on beads were then denatured in sample buffer and separated by SDS-polyacrylamide gel electrophoresis, followed by Western blot (GST-RhoGDI-1 bound RhoA). 20 mg of cell lysates were analyzed for expression of RhoA (total RhoA) by Western blot with anti-Myc antibodies.

Article Snippet: 30 mg of the cleared lysates were separated on a 12.5% SDS-polyacrylamide gel, blotted to nitrocellulose membranes (Gelman), and incubated with antibodies against RhoA (26-C4, Santa Cruz Biotechnology), Rap1A/Krev-1 (C-065, Santa Cruz Biotechnology), or Ha-Ras (C-20, Santa Cruz Biotechnology), respectively.

Techniques: In Vitro, Transfection, Incubation, Purification, Polyacrylamide Gel Electrophoresis, Western Blot, Expressing