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  • 86
    Toronto Research Chemicals podophyllotoxin ppt
    Podophyllotoxin Ppt, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/podophyllotoxin ppt/product/Toronto Research Chemicals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    podophyllotoxin ppt - by Bioz Stars, 2023-02
    86/100 stars
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    93
    MedChemExpress podofilox
    <t>Podofilox</t> inhibits the proliferation of HGC-27 and AGS cells. (A and B) Cell viability was assessed in (A) AGS and (B) HGC-27 cells following incubation with podofilox (0-80 nM) for 48 h. (C and D) Cell proliferation curve was analyzed following incubation with vehicle or 3.4 nM podofilox at the indicated time points. * P<0.05, ** P<0.01 and *** P<0.001 vs. vehicle.
    Podofilox, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/podofilox/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    podofilox - by Bioz Stars, 2023-02
    93/100 stars
      Buy from Supplier

    88
    Biosynth Carbosynth podophyllotoxin ppt
    <t>Podofilox</t> inhibits the proliferation of HGC-27 and AGS cells. (A and B) Cell viability was assessed in (A) AGS and (B) HGC-27 cells following incubation with podofilox (0-80 nM) for 48 h. (C and D) Cell proliferation curve was analyzed following incubation with vehicle or 3.4 nM podofilox at the indicated time points. * P<0.05, ** P<0.01 and *** P<0.001 vs. vehicle.
    Podophyllotoxin Ppt, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/podophyllotoxin ppt/product/Biosynth Carbosynth
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    podophyllotoxin ppt - by Bioz Stars, 2023-02
    88/100 stars
      Buy from Supplier

    86
    Acme Bioscience podofilox
    <t>Podofilox</t> inhibits the proliferation of HGC-27 and AGS cells. (A and B) Cell viability was assessed in (A) AGS and (B) HGC-27 cells following incubation with podofilox (0-80 nM) for 48 h. (C and D) Cell proliferation curve was analyzed following incubation with vehicle or 3.4 nM podofilox at the indicated time points. * P<0.05, ** P<0.01 and *** P<0.001 vs. vehicle.
    Podofilox, supplied by Acme Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/podofilox/product/Acme Bioscience
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    podofilox - by Bioz Stars, 2023-02
    86/100 stars
      Buy from Supplier

    86
    Millipore podofilox
    Microtubule inhibitors limit viral dissemination. MRC5 cells pre-treated with 0.5, 5, 50, and 500 nM of <t>podofilox</t> ( A ); colchicine ( B ); nocodazole ( C ) or ganciclovir (12 μM) ( D , Pulse) were infected with AD169IE2-YFP (MOI 0.1) for 2 h. The cells were washed and analyzed for plaques 7 d p.i. As a control, cells were continuously (Cont.) treated with ganciclovir (12 μM) ( D ). The % Plaques was determined using DMSO-treated cells as 100%. The error bars represent the standard deviation from six replicates.
    Podofilox, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/podofilox/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    podofilox - by Bioz Stars, 2023-02
    86/100 stars
      Buy from Supplier

    Image Search Results


    Podofilox inhibits the proliferation of HGC-27 and AGS cells. (A and B) Cell viability was assessed in (A) AGS and (B) HGC-27 cells following incubation with podofilox (0-80 nM) for 48 h. (C and D) Cell proliferation curve was analyzed following incubation with vehicle or 3.4 nM podofilox at the indicated time points. * P<0.05, ** P<0.01 and *** P<0.001 vs. vehicle.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Podofilox suppresses gastric cancer cell proliferation by regulating cell cycle arrest and the c-Myc/ATG10 axis

    doi: 10.3892/etm.2021.10637

    Figure Lengend Snippet: Podofilox inhibits the proliferation of HGC-27 and AGS cells. (A and B) Cell viability was assessed in (A) AGS and (B) HGC-27 cells following incubation with podofilox (0-80 nM) for 48 h. (C and D) Cell proliferation curve was analyzed following incubation with vehicle or 3.4 nM podofilox at the indicated time points. * P<0.05, ** P<0.01 and *** P<0.001 vs. vehicle.

    Article Snippet: GC cells were seeded into 96-well plates in triplicate at a density of 2,000 cells per well and treated with various concentrations (0, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 and 80 nM; dissolved in DMSO) of podofilox (cat. no. S18-28-5; MedChemExpress).

    Techniques: Incubation

    Podofilox inhibits cell colony formation. (A) AGS and (B) HGC-27 cells were incubated with vehicle or 3.4 nM podofilox for 7 days, and cell colony formation was assessed. ** P<0.01 and *** P<0.001 vs. vehicle.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Podofilox suppresses gastric cancer cell proliferation by regulating cell cycle arrest and the c-Myc/ATG10 axis

    doi: 10.3892/etm.2021.10637

    Figure Lengend Snippet: Podofilox inhibits cell colony formation. (A) AGS and (B) HGC-27 cells were incubated with vehicle or 3.4 nM podofilox for 7 days, and cell colony formation was assessed. ** P<0.01 and *** P<0.001 vs. vehicle.

    Article Snippet: GC cells were seeded into 96-well plates in triplicate at a density of 2,000 cells per well and treated with various concentrations (0, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 and 80 nM; dissolved in DMSO) of podofilox (cat. no. S18-28-5; MedChemExpress).

    Techniques: Incubation

    Podofilox inhibits cell cycle progression of HGC-27 and AGS cells. (A and B) The cell cycle was analyzed in (A) AGS and (B) HGC-27 cells following incubation with vehicle or 3.4 nM podofilox for 48 h. Left, representative images; right, quantification analysis. * P<0.05 and ** P<0.01 vs. vehicle.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Podofilox suppresses gastric cancer cell proliferation by regulating cell cycle arrest and the c-Myc/ATG10 axis

    doi: 10.3892/etm.2021.10637

    Figure Lengend Snippet: Podofilox inhibits cell cycle progression of HGC-27 and AGS cells. (A and B) The cell cycle was analyzed in (A) AGS and (B) HGC-27 cells following incubation with vehicle or 3.4 nM podofilox for 48 h. Left, representative images; right, quantification analysis. * P<0.05 and ** P<0.01 vs. vehicle.

    Article Snippet: GC cells were seeded into 96-well plates in triplicate at a density of 2,000 cells per well and treated with various concentrations (0, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 and 80 nM; dissolved in DMSO) of podofilox (cat. no. S18-28-5; MedChemExpress).

    Techniques: Incubation

    Microarray analysis of dysregulated genes following treatment with podofilox. (A) Heatmap of dysregulated genes in AGS cells treated with vehicle or podofilox. A total of 566 genes were upregulated, and 713 genes were downregulated. Green, downregulated genes; red, upregulated genes. (B) KEGG and HALLMARK pathway enrichment analyses of cell cycle, c-Myc and p53 signaling pathways. KEGG, Kyoto Encyclopedia of Genes and Genomes.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Podofilox suppresses gastric cancer cell proliferation by regulating cell cycle arrest and the c-Myc/ATG10 axis

    doi: 10.3892/etm.2021.10637

    Figure Lengend Snippet: Microarray analysis of dysregulated genes following treatment with podofilox. (A) Heatmap of dysregulated genes in AGS cells treated with vehicle or podofilox. A total of 566 genes were upregulated, and 713 genes were downregulated. Green, downregulated genes; red, upregulated genes. (B) KEGG and HALLMARK pathway enrichment analyses of cell cycle, c-Myc and p53 signaling pathways. KEGG, Kyoto Encyclopedia of Genes and Genomes.

    Article Snippet: GC cells were seeded into 96-well plates in triplicate at a density of 2,000 cells per well and treated with various concentrations (0, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 and 80 nM; dissolved in DMSO) of podofilox (cat. no. S18-28-5; MedChemExpress).

    Techniques: Microarray

    Western blot analysis of dysregulated genes following treatment with podofilox. Immunoblots of HK2, PKM2, p53, c-Myc, ATG10 and GAPDH following incubation with vehicle or 3.4 nM podofilox for 48 h are presented on the left, and the quantitative analysis is presented on the right. * P<0.05 and ** P<0.01 vs. vehicle. HK2, hexokinase 2; PKM2, pyruvate kinase M1/2; ATG10, autophagy-related 10.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Podofilox suppresses gastric cancer cell proliferation by regulating cell cycle arrest and the c-Myc/ATG10 axis

    doi: 10.3892/etm.2021.10637

    Figure Lengend Snippet: Western blot analysis of dysregulated genes following treatment with podofilox. Immunoblots of HK2, PKM2, p53, c-Myc, ATG10 and GAPDH following incubation with vehicle or 3.4 nM podofilox for 48 h are presented on the left, and the quantitative analysis is presented on the right. * P<0.05 and ** P<0.01 vs. vehicle. HK2, hexokinase 2; PKM2, pyruvate kinase M1/2; ATG10, autophagy-related 10.

    Article Snippet: GC cells were seeded into 96-well plates in triplicate at a density of 2,000 cells per well and treated with various concentrations (0, 0.3125, 0.625, 1.25, 2.5, 5, 10, 20, 40 and 80 nM; dissolved in DMSO) of podofilox (cat. no. S18-28-5; MedChemExpress).

    Techniques: Western Blot, Incubation

    Microtubule inhibitors limit viral dissemination. MRC5 cells pre-treated with 0.5, 5, 50, and 500 nM of podofilox ( A ); colchicine ( B ); nocodazole ( C ) or ganciclovir (12 μM) ( D , Pulse) were infected with AD169IE2-YFP (MOI 0.1) for 2 h. The cells were washed and analyzed for plaques 7 d p.i. As a control, cells were continuously (Cont.) treated with ganciclovir (12 μM) ( D ). The % Plaques was determined using DMSO-treated cells as 100%. The error bars represent the standard deviation from six replicates.

    Journal: Viruses

    Article Title: The Microtubule Inhibitor Podofilox Inhibits an Early Entry Step of Human Cytomegalovirus

    doi: 10.3390/v8100295

    Figure Lengend Snippet: Microtubule inhibitors limit viral dissemination. MRC5 cells pre-treated with 0.5, 5, 50, and 500 nM of podofilox ( A ); colchicine ( B ); nocodazole ( C ) or ganciclovir (12 μM) ( D , Pulse) were infected with AD169IE2-YFP (MOI 0.1) for 2 h. The cells were washed and analyzed for plaques 7 d p.i. As a control, cells were continuously (Cont.) treated with ganciclovir (12 μM) ( D ). The % Plaques was determined using DMSO-treated cells as 100%. The error bars represent the standard deviation from six replicates.

    Article Snippet: Podofilox, nocodazole and colchicine were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Infection, Standard Deviation

    Anti-CMV pharmacokinetics is varied among microtubule inhibitors. MRC5 cells were infected with AD169 IE2-YFP and treated with 50 nM podofilox ( A ); 500 nM colchicine ( B ); and 5 μM nocodazole ( C ) at various times relative to infection. Infected cells were analyzed for fluorescent intensity at 18 h p.i. to determine the number of virus-infected cells. The percent infection was normalized to cells receiving DMSO pretreatment as 100%. Columns represent mean of six replicates and error bars show standard error. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Viruses

    Article Title: The Microtubule Inhibitor Podofilox Inhibits an Early Entry Step of Human Cytomegalovirus

    doi: 10.3390/v8100295

    Figure Lengend Snippet: Anti-CMV pharmacokinetics is varied among microtubule inhibitors. MRC5 cells were infected with AD169 IE2-YFP and treated with 50 nM podofilox ( A ); 500 nM colchicine ( B ); and 5 μM nocodazole ( C ) at various times relative to infection. Infected cells were analyzed for fluorescent intensity at 18 h p.i. to determine the number of virus-infected cells. The percent infection was normalized to cells receiving DMSO pretreatment as 100%. Columns represent mean of six replicates and error bars show standard error. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Podofilox, nocodazole and colchicine were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Infection

    Podofilox inhibits CMV entry post binding. MRC5 cells or virus were pretreated with DMSO, podofilox (50 nM), colchicine (500 nM), nocodazole (5 μM), and JL122 (250 nM) for 1 h (or virus was treated for 1 h in the case of JL122) and infected with AD169 WT for 2 h at either 4 °C ( A ) or 37 °C ( B ) and analyzed for the presence of viral DNA by qPCR; ( A ) Bound Virus (%) and ( B ) Internalized Virus (%) values were determined using the relative number of virus genomes (see Materials and Methods) from DMSO treated cells as 100%. Graph represents the average of three separate experiments each performed in triplicate, error bars correspond to standard error. ** p < 0.01, *** p < 0.001.

    Journal: Viruses

    Article Title: The Microtubule Inhibitor Podofilox Inhibits an Early Entry Step of Human Cytomegalovirus

    doi: 10.3390/v8100295

    Figure Lengend Snippet: Podofilox inhibits CMV entry post binding. MRC5 cells or virus were pretreated with DMSO, podofilox (50 nM), colchicine (500 nM), nocodazole (5 μM), and JL122 (250 nM) for 1 h (or virus was treated for 1 h in the case of JL122) and infected with AD169 WT for 2 h at either 4 °C ( A ) or 37 °C ( B ) and analyzed for the presence of viral DNA by qPCR; ( A ) Bound Virus (%) and ( B ) Internalized Virus (%) values were determined using the relative number of virus genomes (see Materials and Methods) from DMSO treated cells as 100%. Graph represents the average of three separate experiments each performed in triplicate, error bars correspond to standard error. ** p < 0.01, *** p < 0.001.

    Article Snippet: Podofilox, nocodazole and colchicine were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Binding Assay, Infection

    Podofilox blocks a latter step during CMV entry. ( A ) MRC5 cells infected with AD169 IE2-YFP (MOI 3) at 4 °C were either washed with either a pH 3.0 buffer (white bars) or pH 7.0 buffer (gray bars) (Binding) or shifted to 37 °C for 1 h and then washed with a pH 3.0 or pH 7.0 buffer (Penetration); ( B ) MRC5 cells pretreated with podofilox were infected with AD169 IE2-YFP (MOI 3) at 4 °C, shifted to 37 °C for 1 h and then washed with a pH 3.0 or pH 7.0 buffer. For both ( A ) and ( B ); virus infection was analyzed an Acumen X3 cytometer (18 h p.i.) and the % infection was determined using DMSO treated pH 7.0 washed cells as 100% infection. The error bars correspond to standard error of six replicates.

    Journal: Viruses

    Article Title: The Microtubule Inhibitor Podofilox Inhibits an Early Entry Step of Human Cytomegalovirus

    doi: 10.3390/v8100295

    Figure Lengend Snippet: Podofilox blocks a latter step during CMV entry. ( A ) MRC5 cells infected with AD169 IE2-YFP (MOI 3) at 4 °C were either washed with either a pH 3.0 buffer (white bars) or pH 7.0 buffer (gray bars) (Binding) or shifted to 37 °C for 1 h and then washed with a pH 3.0 or pH 7.0 buffer (Penetration); ( B ) MRC5 cells pretreated with podofilox were infected with AD169 IE2-YFP (MOI 3) at 4 °C, shifted to 37 °C for 1 h and then washed with a pH 3.0 or pH 7.0 buffer. For both ( A ) and ( B ); virus infection was analyzed an Acumen X3 cytometer (18 h p.i.) and the % infection was determined using DMSO treated pH 7.0 washed cells as 100% infection. The error bars correspond to standard error of six replicates.

    Article Snippet: Podofilox, nocodazole and colchicine were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Infection, Binding Assay, Cytometry

    Podofilox’s effect on CMV infection is reversible. MRC5 cells pretreated with 50 nM podofilox were infected with AD169 IE2-YFP and analyzed for fluorescent intensity at 18 h p.i. to determine the number of virus-infected cells. The percentage infection was normalized to cells receiving DMSO pretreatment as 100%. Columns represent average of six replicates and error bars correspond to standard error. ** p < 0.01, *** p < 0.001.

    Journal: Viruses

    Article Title: The Microtubule Inhibitor Podofilox Inhibits an Early Entry Step of Human Cytomegalovirus

    doi: 10.3390/v8100295

    Figure Lengend Snippet: Podofilox’s effect on CMV infection is reversible. MRC5 cells pretreated with 50 nM podofilox were infected with AD169 IE2-YFP and analyzed for fluorescent intensity at 18 h p.i. to determine the number of virus-infected cells. The percentage infection was normalized to cells receiving DMSO pretreatment as 100%. Columns represent average of six replicates and error bars correspond to standard error. ** p < 0.01, *** p < 0.001.

    Article Snippet: Podofilox, nocodazole and colchicine were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Infection

    Podofilox efficacy is dependent on CMV strain and cell type. The fluorescent signal from TB40/E WT -infected MRC5 cells ( A ) and TB40/E WT -infected ARPE-19 cells ( B ) pretreated with varying doses of podofilox and stained at 18 h p.i. with murine anti-IE1 conjugated to Alexa fluorophore 488 was analyzed with an Acumen e X3 cytometer. The number of virus-infected cells were used to determine percent infection with cells receiving DMSO pretreatment as 100% infection. Non-linear regression analysis was performed in Prism5. Points represent the mean of six replicates and error bars represent the standard error.

    Journal: Viruses

    Article Title: The Microtubule Inhibitor Podofilox Inhibits an Early Entry Step of Human Cytomegalovirus

    doi: 10.3390/v8100295

    Figure Lengend Snippet: Podofilox efficacy is dependent on CMV strain and cell type. The fluorescent signal from TB40/E WT -infected MRC5 cells ( A ) and TB40/E WT -infected ARPE-19 cells ( B ) pretreated with varying doses of podofilox and stained at 18 h p.i. with murine anti-IE1 conjugated to Alexa fluorophore 488 was analyzed with an Acumen e X3 cytometer. The number of virus-infected cells were used to determine percent infection with cells receiving DMSO pretreatment as 100% infection. Non-linear regression analysis was performed in Prism5. Points represent the mean of six replicates and error bars represent the standard error.

    Article Snippet: Podofilox, nocodazole and colchicine were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Infection, Staining, Cytometry

    Podofilox inhibition of diverse enveloped viruses. MRC5 cells pretreated podofilox were infected with various reporter viruses and analyzed for GFP fluorescence (HSV1 GFP , VSV GFP NDV GFP , and SeV GFP ) or luciferase activity (IAV Luc ). The number of infected cells was determined using an Acumen e X3 cytometer for HSV1 GFP ( A , 24 h p.i.); VSV GFP ( B , 8 h p.i.); NDV GFP ( D , 24 h p.i.) and SeV GFP ( E , 24 h p.i.); ForIAV Luc ( C ); the level of luciferase activity was quantified at 24 h p.i. on the BioTek Cytation 3 imaging reader. The % infection was determined using DMSO-treated cells as 100% infection. Non-linear regression analysis was performed in Prism5. Points represent the mean of six replicates and error bars represent the standard error.

    Journal: Viruses

    Article Title: The Microtubule Inhibitor Podofilox Inhibits an Early Entry Step of Human Cytomegalovirus

    doi: 10.3390/v8100295

    Figure Lengend Snippet: Podofilox inhibition of diverse enveloped viruses. MRC5 cells pretreated podofilox were infected with various reporter viruses and analyzed for GFP fluorescence (HSV1 GFP , VSV GFP NDV GFP , and SeV GFP ) or luciferase activity (IAV Luc ). The number of infected cells was determined using an Acumen e X3 cytometer for HSV1 GFP ( A , 24 h p.i.); VSV GFP ( B , 8 h p.i.); NDV GFP ( D , 24 h p.i.) and SeV GFP ( E , 24 h p.i.); ForIAV Luc ( C ); the level of luciferase activity was quantified at 24 h p.i. on the BioTek Cytation 3 imaging reader. The % infection was determined using DMSO-treated cells as 100% infection. Non-linear regression analysis was performed in Prism5. Points represent the mean of six replicates and error bars represent the standard error.

    Article Snippet: Podofilox, nocodazole and colchicine were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Inhibition, Infection, Fluorescence, Luciferase, Activity Assay, Cytometry, Imaging

    Fluorescent microscopy of MRC5 cells treated with microtubule inhibitors for 1 h. MRC5 cells were treated for 1 h with DMSO ( A ); 50 nM podofilox ( B ); 500 nM colchicine ( C ); or 5 μM nocodazole ( D ). Cells were stained with murine anti-tubulin antibody and visualized anti-mouse antibody conjugated to Alexa 488 (green) and nuclei stained with Hoescht reagent (blue). Scale bar is 25 μm.

    Journal: Viruses

    Article Title: The Microtubule Inhibitor Podofilox Inhibits an Early Entry Step of Human Cytomegalovirus

    doi: 10.3390/v8100295

    Figure Lengend Snippet: Fluorescent microscopy of MRC5 cells treated with microtubule inhibitors for 1 h. MRC5 cells were treated for 1 h with DMSO ( A ); 50 nM podofilox ( B ); 500 nM colchicine ( C ); or 5 μM nocodazole ( D ). Cells were stained with murine anti-tubulin antibody and visualized anti-mouse antibody conjugated to Alexa 488 (green) and nuclei stained with Hoescht reagent (blue). Scale bar is 25 μm.

    Article Snippet: Podofilox, nocodazole and colchicine were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Microscopy, Staining