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Image Search Results
Journal: The Journal of Clinical Investigation
Article Title: CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease
doi: 10.1172/JCI99507
Figure Lengend Snippet: MRL.lpr mice were injected i.p. with free KN93 (10 μg/wk), anti-podocin or anti-nephrin antibody–coated KN93-loaded nlg (10 μg of KN93/week), or empty nlg at from 8 to 16 weeks of age (n = 5–7 mice in each group). (A) Urine albumin/creatinine (Alb/Cre) ratio. Urine samples were obtained biweekly, and albumin and creatinine levels were determined by ELISA. Error bars represent mean ± SEM. *P < 0.05, 2-way ANOVA with Bonferroni’s post test. (B and C) Representative images of glomeruli from 16-week- old MRL.lpr mice treated with anti-nephrin antibody–coated empty nlg or KN93-loaded nlg. PAS (B) and Masson’s trichrome staining (C) are shown. The arrows point to the crescent. Scale bars: 50 μm. (D) Mean histological scores in the kidneys of mice from the indicated treatment groups (n = 5 in each group). *P < 0.05; **P < 0.01, Student’s t test. (E and F) Electron microscopic images of a glomerulus from a mouse treated with anti-nephrin empty nlg (left) showing diffuse podocyte foot process (FP) effacement with slit diaphragm occlusion (arrows) and subepithelial dense deposits (asterisks) and a mouse treated with anti-nephrin KN93-loaded nlg (right), which shows podocytes with normal foot processes and slit diaphragms and no deposits in the basement membrane. Original magnification, ×8,000 (E); ×30,000 (F). Three glomeruli were evaluated in each of 3 mice in each experimental condition. (G) C3 and IgG deposition was significantly suppressed in the glomeruli of MRL.lpr mice treated with KN93 targeted to podocytes, as indicated. Kidney sections from MRL/MpJ (16 weeks of age) mice are shown as controls (n = 5 mice in each group). (H) Nephrin (green) and synaptopodin (red) expression detected by immunofluorescence. Scale bars: 50 μm. (I) The fluorescence intensity of nephrin and synaptopodin quantified in glomeruli from mice subjected to the indicated treatments. ****P < 0.0001, Student’s t test. (J) Nphs1, Nphs2, and Synpo expression in glomeruli from mice treated with anti-nephrin–coated empty or KN93-loaded nlg. Results were normalized to the expression of GAPDH (n = 5 in each group). ****P < 0.0001, Student’s t test.
Article Snippet: Isolated cells were stained for flow cytometry with antibodies against nephrin (bs-10233R-A488, Bioss Antibodies) and
Techniques: Injection, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Immunofluorescence, Fluorescence