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Image Search Results
Journal: bioRxiv
Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir
doi: 10.1101/2024.12.06.627250
Figure Lengend Snippet: (A-D) HIV-1 virions were produced by transfecting HEK293T cells (producer cells) with indicated concentrations of full-length WT pNL4.3. Indicated concentrations of LEN or DMSO control were added to HEK293T cells, and viruses isolated by ultracentrifugation through 20% sucrose cushions were used to infect HeLa TZM-bl cells (Target cells). After 48 h of infection, luciferase activity was measured to determine EC 50 values of LEN. The averaged data (+/− SD) from three independent experiments are shown.
Article Snippet: Viruses were generated by transfecting HEK293T cells (10 7 plated in 15 cm plates the previous day) with 30 µg
Techniques: Produced, Control, Isolation, Infection, Luciferase, Activity Assay
Journal: bioRxiv
Article Title: The primary mechanism for highly potent inhibition of HIV-1 maturation by lenacapavir
doi: 10.1101/2024.12.06.627250
Figure Lengend Snippet: (A) The experimental design. The same virus preparation was used for three different assays: 1) LC-MS/MS to determine LEN amounts bound to virions, 2) p24 ELISA to determine CA amounts; and 3) infectivity of the viruses produced in the presence of LEN or DMSO control in HeLa TZM-bl cells. (B) Representative LC-MS/MS results of viruses produced in the presence of LEN (the top panel), the identical concentration of LEN added to HEK293T cells in the absence of pNL4.3 (the middle panel), and the control of the medium without LEN or the virus (the bottom panel). (C) Infectivity of the viruses at indicated [LEN]:[CA] ratios or DMSO control. The [LEN]:[CA] ratios were measured using p24 ELISA and LC-MS/MS results for the same virus preparations.
Article Snippet: Viruses were generated by transfecting HEK293T cells (10 7 plated in 15 cm plates the previous day) with 30 µg
Techniques: Virus, Liquid Chromatography with Mass Spectroscopy, Enzyme-linked Immunosorbent Assay, Infection, Produced, Control, Concentration Assay