pmsp12 Search Results


pmsp12  (Addgene inc)
93
Addgene inc pmsp12
Pmsp12, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmsp12/product/Addgene inc
Average 93 stars, based on 1 article reviews
pmsp12 - by Bioz Stars, 2026-02
93/100 stars
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mtb aux expressing dsred  (Addgene inc)
93
Addgene inc mtb aux expressing dsred
Macrophages more readily phagocytose Zn 2+ -limited Mtb than Zn 2+ -replete Mtb. (A) Intracellular CFUs of Aux-ZRM and Aux-ZLM in RAW 264.7 cells at 4 and 24 hpi. (B) The percentage and (C) relative bacterial burden of RAW 264.7 macrophages infected with <t>DsRed-expressing</t> Aux-ZRM and Aux-ZLM at 4 and 24 hpi, as determined by flow cytometry. (D) Intracellular CFUs of Rv-ZRM and Rv-ZLM in murine BMDMs at 4 hpi. (E) Intracellular CFUs of Aux-ZRM and Aux-ZLM in THP-1 macrophages at 4 hpi. (F) The percentage and (G) relative bacterial burden of THP-1 macrophages infected with GFP-expressing Aux-ZRM and Aux-ZLM at 2 hpi, as determined by flow cytometry. Bacterial burden is relative to the burden of macrophages infected with Aux-ZRM. The error bars are the standard deviations of six replicates. Representative data from at least two independent experiments are shown. **: P -value < 0.01. ***: P -value < 0.001. ****: P -value < 0.0001. CFUs: colony forming units; Aux-ZRM and Aux-ZLM: Mtb mc 2 6206 grown in Zn 2+ -replete or limited media, respectively; Rv-ZRM and Rv-ZLM: Mtb H37Rv grown in Zn 2+ -replete or limited media, respectively; BMDM: bone marrow-derived macrophages. MΦ: macrophage. a.u.: arbitrary units.
Mtb Aux Expressing Dsred, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mtb aux expressing dsred/product/Addgene inc
Average 93 stars, based on 1 article reviews
mtb aux expressing dsred - by Bioz Stars, 2026-02
93/100 stars
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ptec27 plasmid pmsp12::tdtomato  (Deepak Inc)
90
Deepak Inc ptec27 plasmid pmsp12::tdtomato
Macrophages more readily phagocytose Zn 2+ -limited Mtb than Zn 2+ -replete Mtb. (A) Intracellular CFUs of Aux-ZRM and Aux-ZLM in RAW 264.7 cells at 4 and 24 hpi. (B) The percentage and (C) relative bacterial burden of RAW 264.7 macrophages infected with <t>DsRed-expressing</t> Aux-ZRM and Aux-ZLM at 4 and 24 hpi, as determined by flow cytometry. (D) Intracellular CFUs of Rv-ZRM and Rv-ZLM in murine BMDMs at 4 hpi. (E) Intracellular CFUs of Aux-ZRM and Aux-ZLM in THP-1 macrophages at 4 hpi. (F) The percentage and (G) relative bacterial burden of THP-1 macrophages infected with GFP-expressing Aux-ZRM and Aux-ZLM at 2 hpi, as determined by flow cytometry. Bacterial burden is relative to the burden of macrophages infected with Aux-ZRM. The error bars are the standard deviations of six replicates. Representative data from at least two independent experiments are shown. **: P -value < 0.01. ***: P -value < 0.001. ****: P -value < 0.0001. CFUs: colony forming units; Aux-ZRM and Aux-ZLM: Mtb mc 2 6206 grown in Zn 2+ -replete or limited media, respectively; Rv-ZRM and Rv-ZLM: Mtb H37Rv grown in Zn 2+ -replete or limited media, respectively; BMDM: bone marrow-derived macrophages. MΦ: macrophage. a.u.: arbitrary units.
Ptec27 Plasmid Pmsp12/Tdtomato, supplied by Deepak Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ptec27 plasmid pmsp12::tdtomato/product/Deepak Inc
Average 90 stars, based on 1 article reviews
ptec27 plasmid pmsp12::tdtomato - by Bioz Stars, 2026-02
90/100 stars
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mycobacterium tuberculosis pmsp12:: gfp  (MiddleBrook Pharmaceuticals)
90
MiddleBrook Pharmaceuticals mycobacterium tuberculosis pmsp12:: gfp
Macrophages more readily phagocytose Zn 2+ -limited Mtb than Zn 2+ -replete Mtb. (A) Intracellular CFUs of Aux-ZRM and Aux-ZLM in RAW 264.7 cells at 4 and 24 hpi. (B) The percentage and (C) relative bacterial burden of RAW 264.7 macrophages infected with <t>DsRed-expressing</t> Aux-ZRM and Aux-ZLM at 4 and 24 hpi, as determined by flow cytometry. (D) Intracellular CFUs of Rv-ZRM and Rv-ZLM in murine BMDMs at 4 hpi. (E) Intracellular CFUs of Aux-ZRM and Aux-ZLM in THP-1 macrophages at 4 hpi. (F) The percentage and (G) relative bacterial burden of THP-1 macrophages infected with GFP-expressing Aux-ZRM and Aux-ZLM at 2 hpi, as determined by flow cytometry. Bacterial burden is relative to the burden of macrophages infected with Aux-ZRM. The error bars are the standard deviations of six replicates. Representative data from at least two independent experiments are shown. **: P -value < 0.01. ***: P -value < 0.001. ****: P -value < 0.0001. CFUs: colony forming units; Aux-ZRM and Aux-ZLM: Mtb mc 2 6206 grown in Zn 2+ -replete or limited media, respectively; Rv-ZRM and Rv-ZLM: Mtb H37Rv grown in Zn 2+ -replete or limited media, respectively; BMDM: bone marrow-derived macrophages. MΦ: macrophage. a.u.: arbitrary units.
Mycobacterium Tuberculosis Pmsp12/ Gfp, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mycobacterium tuberculosis pmsp12:: gfp/product/MiddleBrook Pharmaceuticals
Average 90 stars, based on 1 article reviews
mycobacterium tuberculosis pmsp12:: gfp - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Macrophages more readily phagocytose Zn 2+ -limited Mtb than Zn 2+ -replete Mtb. (A) Intracellular CFUs of Aux-ZRM and Aux-ZLM in RAW 264.7 cells at 4 and 24 hpi. (B) The percentage and (C) relative bacterial burden of RAW 264.7 macrophages infected with DsRed-expressing Aux-ZRM and Aux-ZLM at 4 and 24 hpi, as determined by flow cytometry. (D) Intracellular CFUs of Rv-ZRM and Rv-ZLM in murine BMDMs at 4 hpi. (E) Intracellular CFUs of Aux-ZRM and Aux-ZLM in THP-1 macrophages at 4 hpi. (F) The percentage and (G) relative bacterial burden of THP-1 macrophages infected with GFP-expressing Aux-ZRM and Aux-ZLM at 2 hpi, as determined by flow cytometry. Bacterial burden is relative to the burden of macrophages infected with Aux-ZRM. The error bars are the standard deviations of six replicates. Representative data from at least two independent experiments are shown. **: P -value < 0.01. ***: P -value < 0.001. ****: P -value < 0.0001. CFUs: colony forming units; Aux-ZRM and Aux-ZLM: Mtb mc 2 6206 grown in Zn 2+ -replete or limited media, respectively; Rv-ZRM and Rv-ZLM: Mtb H37Rv grown in Zn 2+ -replete or limited media, respectively; BMDM: bone marrow-derived macrophages. MΦ: macrophage. a.u.: arbitrary units.

Journal: Infection and Immunity

Article Title: Zinc-limited Mycobacterium tuberculosis stimulate distinct responses in macrophages compared with standard zinc-replete bacteria

doi: 10.1128/iai.00578-24

Figure Lengend Snippet: Macrophages more readily phagocytose Zn 2+ -limited Mtb than Zn 2+ -replete Mtb. (A) Intracellular CFUs of Aux-ZRM and Aux-ZLM in RAW 264.7 cells at 4 and 24 hpi. (B) The percentage and (C) relative bacterial burden of RAW 264.7 macrophages infected with DsRed-expressing Aux-ZRM and Aux-ZLM at 4 and 24 hpi, as determined by flow cytometry. (D) Intracellular CFUs of Rv-ZRM and Rv-ZLM in murine BMDMs at 4 hpi. (E) Intracellular CFUs of Aux-ZRM and Aux-ZLM in THP-1 macrophages at 4 hpi. (F) The percentage and (G) relative bacterial burden of THP-1 macrophages infected with GFP-expressing Aux-ZRM and Aux-ZLM at 2 hpi, as determined by flow cytometry. Bacterial burden is relative to the burden of macrophages infected with Aux-ZRM. The error bars are the standard deviations of six replicates. Representative data from at least two independent experiments are shown. **: P -value < 0.01. ***: P -value < 0.001. ****: P -value < 0.0001. CFUs: colony forming units; Aux-ZRM and Aux-ZLM: Mtb mc 2 6206 grown in Zn 2+ -replete or limited media, respectively; Rv-ZRM and Rv-ZLM: Mtb H37Rv grown in Zn 2+ -replete or limited media, respectively; BMDM: bone marrow-derived macrophages. MΦ: macrophage. a.u.: arbitrary units.

Article Snippet: The fluorescent strain of Mtb-Aux expressing DsRed (pMSP12::DsRed, Addgene plasmid # 30171) and GFP (Live-Dead reporter; a gift from Dr. Christopher Sassetti) were used for the flow cytometry assays and confocal microscopy when indicated ( , ).

Techniques: Infection, Expressing, Flow Cytometry, Derivative Assay