Journal: Oncotarget

Article Title: Functional role of DNA mismatch repair gene PMS2 in prostate cancer cells

doi:

Figure Lengend Snippet: Prostatic cell lines were grown in culture dishes for two days and underwent Western analyses. Shown is a representative immunoblot of PMS2 protein expression in DU145 and normal prostate epithelial PWR-1E and RWPE-1 cells. GAPDH was used as loading control.

Article Snippet: Monoclonal antibody against PMS2 was purchased from Origene Technologies (Rockville, MD) and monoclonal antibody against GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA) was used to confirm equal loading.

Techniques: Western Blot, Expressing, Control

Journal: Oncotarget

Article Title: Functional role of DNA mismatch repair gene PMS2 in prostate cancer cells

doi:

Figure Lengend Snippet: A. Ectopic expression of PMS2. DU145 cells stably transfected with either PMS2 or empty vector (pCMV) along with mock (parental DU145 cells treated with transfection reagent alone) were grown for 48 hours and underwent Western analyses. GAPDH was used as loading control. Note: Clone #2 (not shown) displayed a band similar in intensity to #1 . B. Cell proliferation as analyzed by the MTS cell proliferation assay 72 hours after plating cells. Results are expressed as % and normalized to pCMV control. C. Cell migration as measured by wound healing assay. A wound was formed by scraping culture dishes using a pipet tip and closure measured after 24 hours. Left : Representative images of wound healing assay are shown. Right : Migration expressed as % closure of wound. D. Cell invasiveness as measured using Matrigel. Cells were placed onto transwell membrane and allowed to invade for 24 hours. Left : Representative images of invading cells are shown. Right : Cell invasiveness as measured by absorbance (Abs) at 560 nm. Data are presented as mean ± SEM of at least three experiments; ** P < 0.01 PMS2 versus pCMV.

Article Snippet: Monoclonal antibody against PMS2 was purchased from Origene Technologies (Rockville, MD) and monoclonal antibody against GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA) was used to confirm equal loading.

Techniques: Expressing, Stable Transfection, Transfection, Plasmid Preparation, Western Blot, Control, Proliferation Assay, Migration, Wound Healing Assay, Membrane

Journal: Oncotarget

Article Title: Functional role of DNA mismatch repair gene PMS2 in prostate cancer cells

doi:

Figure Lengend Snippet: Athymic nude mice were injected subcutaneously with stable PMS2 or pCMV-transfected DU145 cells and growth determined after 35 days. Left : Representative image of tumors in mice. Right : Tumor size (mm 3 ). Data are presented as mean ± SEM of five mice per group; * P < 0.05 PMS2 versus pCMV.

Article Snippet: Monoclonal antibody against PMS2 was purchased from Origene Technologies (Rockville, MD) and monoclonal antibody against GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA) was used to confirm equal loading.

Techniques: Injection, Transfection

Journal: Oncotarget

Article Title: Functional role of DNA mismatch repair gene PMS2 in prostate cancer cells

doi:

Figure Lengend Snippet: Stable PMS2-expressing and vector control DU145 cells were grown for 72 hours and apoptosis was measured by flow cytometric analyses. Top : Representative biparametric histogram showing cell population in early (bottom right quadrant) and late (top right quadrant) apoptotic, and viable (bottom left quadrant) states for each treatment. Bottom : Total apoptosis %. Bar graph is mean ± SEM of three experiments; ** P < 0.01, * P < 0.05 PMS2 versus pCMV.

Article Snippet: Monoclonal antibody against PMS2 was purchased from Origene Technologies (Rockville, MD) and monoclonal antibody against GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA) was used to confirm equal loading.

Techniques: Expressing, Plasmid Preparation, Control

Journal: Oncotarget

Article Title: Functional role of DNA mismatch repair gene PMS2 in prostate cancer cells

doi:

Figure Lengend Snippet: Apoptosis-related genes increased or decreased 2-fold or greater due to expression of PMS2 in DU145 clone #1 cells

Article Snippet: Monoclonal antibody against PMS2 was purchased from Origene Technologies (Rockville, MD) and monoclonal antibody against GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA) was used to confirm equal loading.

Techniques: Expressing

Journal: Oncotarget

Article Title: Functional role of DNA mismatch repair gene PMS2 in prostate cancer cells

doi:

Figure Lengend Snippet: Stable PMS2-expressing and vector control DU145 cells were grown and expression of A. TMS1 and B. BCL2A1 was determined by real-time PCR. Levels are normalized to vector control. Data are presented as mean ± SEM of three experiments; ** P < 0.01 PMS2 versus pCMV.

Article Snippet: Monoclonal antibody against PMS2 was purchased from Origene Technologies (Rockville, MD) and monoclonal antibody against GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA) was used to confirm equal loading.

Techniques: Expressing, Plasmid Preparation, Control, Real-time Polymerase Chain Reaction

Journal: World Journal of Surgical Oncology

Article Title: Clinicopathological and prognostic significance of SWI/SNF complex subunits in undifferentiated gastric carcinoma

doi: 10.1186/s12957-022-02847-0

Figure Lengend Snippet: Summary of antibody information

Article Snippet: PMS2 , EP51 , Rabbit , working solution , Origene.

Techniques:

Journal: Journal of Cytology

Article Title: Screening of Endometrial Cancer Related to Lynch Syndrome in China by Suction Curettage-Based Cytology and Histology: A Retrospective Study

doi: 10.4103/joc.joc_39_22

Figure Lengend Snippet: IHC staining in endometrial cancer (a) MLH1 negative, (b) MSH2 positive, (c) MSH6 positive, and (d) PMS2 negative in microtissue of endometrial cancer (X200)

Article Snippet: The MMR monoclonal antibodies, MLH1 (mouse monoclonal antibody 1:100), MSH2 (rabbit monoclonal antibody 1:150), MSH6 (rabbit monoclonal antibody 1:150), and PMS2 (rabbit monoclonal antibody 1:100), were purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.

Techniques: Immunohistochemistry