Journal: Journal of Neuroinflammation

Article Title: Involvement of neuronal IL-1β in acquired brain lesions in a rat model of neonatal encephalopathy

doi: 10.1186/1742-2094-10-110

Figure Lengend Snippet: List and features of antibodies

Article Snippet: Anti-PMN , Cedarlane - CLAD51140 , 1:200.

Techniques:

Journal: iScience

Article Title: Differential Roles of the Calcium Ion Channel TRPV4 in Host Responses to Mycobacterium tuberculosis Early and Late in Infection.

doi: 10.1016/j.isci.2020.101206

Figure Lengend Snippet: Figure 7. Expression of Neutrophil Elastase in M. tuberculosis-Infected Mouse Lung (A–C) (A and B) WT and Trpv4/mice were infected with M. tuberculosis (100 CFU) via aerosol, and 4-mm cryosections of lung tissue samples were prepared after days 55 and 150 p.i. Tissue sections were stained by fluorescence immunohistochemistry assay for neutrophil elastase (green). Images were acquired from three different zones of infection (field) from each mouse; Scale bar=20 mm). Mean fluorescence intensity (MFI) of neutrophil elastase staining was quantified by using Fiji ImageJ software. (C) Human lung tissue sections of patients with TB were stained for TRPV4 showing positive signals in polymorphonuclear neutrophils in a blood vessel at the site of infection (Scale bar of R1, R2 = 20 mm).

Article Snippet: Primary antibodies directed against Trpv4 (#ACC034, Alomone lab), Rab5 (#GTX108605, GeneTex), neutrophil elastase (#51-862, proSci- ELAN)were used in this study.

Techniques: Expressing, Infection, Aerosol, Staining, Immunohistochemistry, Software