Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Conformational Antibodies to Proteolipid Protein-1 and Its Peripheral Isoform DM20 in Patients With CNS Autoimmune Demyelinating Disorders

doi: 10.1212/NXI.0000000000200359

Figure Lengend Snippet: (A) Monoclonal antibodies against different epitopes of PLP1 and serum from a patient with ADD label the surface of live HEK293T cells transfected with PLP1-EGFP-tag (green). Merged images show colocalization of the staining (orange). No binding is observed with the MOG 8-18C5 monoclonal antibody or with serum from a healthy control. (B) Structure of PLP1. Colored aminoacidic sequences identify the different epitopes recognized by the monoclonal antibodies (yellow = PLP1 50-69 ; green = PLP1 178-191 , and blue = PLP1 200-219 ) or their intracellular portion, which is absent in the DM20 protein isoform (red). (C) Serum of a PLP1-IgG positive patient (green) binds to the myelinated areas (cerebellum, striatum and hippocampus) of lightly fixed rat brain sagittal slices (indirect immunofluorescence). PLP1 monoclonal antibody (red) binds to the same regions, colocalizing with patients' serum (yellow). DAPI stains cell nuclei. Scale bar: 50 µm. (D) Serum from the same patient stains both PLP1-CBA and TBA on lightly fixed rat brain (left column); unmodified staining after preadsorption on MOG-transfected cells (central column); abolition of the binding after preadsorption on PLP1-transfected cells on both CBA and TBA (right column). Created in BioRender. Gastaldi, M. (2024) BioRender.com/v24o621 . ADD = autoimmune demyelinating disorder; CBA = cell-based assay; MOG = myelin oligodendrocyte glycoprotein; PLP1 = proteolipid protein-1; TBA = tissue-based assay.

Article Snippet: Live cell-based assays were implemented using the following plasmids: EGFP-tagged full-length human MOG and untagged rat full-length MOG (kind gift of Prof M. Reindl); GFP-tagged human PLP1 (code: RG218616, Origene); untagged human PLP1 (code: SC119823, Origene); untagged human DM20 (a splicing variant of PLP1, code: SC121107, Origene).

Techniques: Bioprocessing, Transfection, Staining, Binding Assay, Control, Immunofluorescence, Cell Based Assay

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Conformational Antibodies to Proteolipid Protein-1 and Its Peripheral Isoform DM20 in Patients With CNS Autoimmune Demyelinating Disorders

doi: 10.1212/NXI.0000000000200359

Figure Lengend Snippet: (A) Serum of a patient with PLP1-IgG binds to DM20-transfected CBA (green) and colocalizes with a PLP1 mAb (red) directed against an extracellular loop of the protein (top row). No binding is observed with serum from a HC (bottom row). (B) Binding to both PLP1- and DM20-transfected cells (green) is observed with both PLP1mAb (first row) and serum from a patient with CNS+PNS ADD (pt#1; second row). Conversely, serum from a patient with CNS ADD binds exclusively to PLP1-transfected cells and not to DM20-transfected cells (third row). No binding is observed with serum from a HC (bottom row). DAPI stains the nuclei. (C) Heatmap representing fluorescence intensity of the available samples (n = 36) in either PLP1 or DM20 CBA measured with a semiquantitative score. All PLP1-IgG–positive patients with CNS + PNS ADD, unlike patients with other disease groups, were positive on both PLP1 and DM20 CBA. Scale bars: 10 µm. ADD = autoimmune demyelinating disorder; CBA = cell-based assay; Ig = immunoglobulin; HC = healthy control; mAbs = monoclonal antibodies; PNS = peripheral nervous system; PLP1 = proteolipid protein-1; Pt = patient.

Article Snippet: Live cell-based assays were implemented using the following plasmids: EGFP-tagged full-length human MOG and untagged rat full-length MOG (kind gift of Prof M. Reindl); GFP-tagged human PLP1 (code: RG218616, Origene); untagged human PLP1 (code: SC119823, Origene); untagged human DM20 (a splicing variant of PLP1, code: SC121107, Origene).

Techniques: Transfection, Binding Assay, Fluorescence, Cell Based Assay, Control, Bioprocessing

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Conformational Antibodies to Proteolipid Protein-1 and Its Peripheral Isoform DM20 in Patients With CNS Autoimmune Demyelinating Disorders

doi: 10.1212/NXI.0000000000200359

Figure Lengend Snippet: (A) Monoclonal antibodies against different epitopes of PLP1 and serum from a patient with ADD label the surface of live HEK293T cells transfected with PLP1-EGFP-tag (green). Merged images show colocalization of the staining (orange). No binding is observed with the MOG 8-18C5 monoclonal antibody or with serum from a healthy control. (B) Structure of PLP1. Colored aminoacidic sequences identify the different epitopes recognized by the monoclonal antibodies (yellow = PLP1 50-69 ; green = PLP1 178-191 , and blue = PLP1 200-219 ) or their intracellular portion, which is absent in the DM20 protein isoform (red). (C) Serum of a PLP1-IgG positive patient (green) binds to the myelinated areas (cerebellum, striatum and hippocampus) of lightly fixed rat brain sagittal slices (indirect immunofluorescence). PLP1 monoclonal antibody (red) binds to the same regions, colocalizing with patients' serum (yellow). DAPI stains cell nuclei. Scale bar: 50 µm. (D) Serum from the same patient stains both PLP1-CBA and TBA on lightly fixed rat brain (left column); unmodified staining after preadsorption on MOG-transfected cells (central column); abolition of the binding after preadsorption on PLP1-transfected cells on both CBA and TBA (right column). Created in BioRender. Gastaldi, M. (2024) BioRender.com/v24o621 . ADD = autoimmune demyelinating disorder; CBA = cell-based assay; MOG = myelin oligodendrocyte glycoprotein; PLP1 = proteolipid protein-1; TBA = tissue-based assay.

Article Snippet: Live cell-based assays were implemented using the following plasmids: EGFP-tagged full-length human MOG and untagged rat full-length MOG (kind gift of Prof M. Reindl); GFP-tagged human PLP1 (code: RG218616, Origene); untagged human PLP1 (code: SC119823, Origene); untagged human DM20 (a splicing variant of PLP1, code: SC121107, Origene).

Techniques: Bioprocessing, Transfection, Staining, Binding Assay, Control, Immunofluorescence, Cell Based Assay

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Conformational Antibodies to Proteolipid Protein-1 and Its Peripheral Isoform DM20 in Patients With CNS Autoimmune Demyelinating Disorders

doi: 10.1212/NXI.0000000000200359

Figure Lengend Snippet: Patients from (A) a retrospective exploratory cohort and (B) a prospective validation cohort were tested. (C) Proportions of PLP1-IgG–positive patients within the diagnostic subgroups of both the exploratory and validation cohorts. *Seventy-three of 824 patients without sufficient clinical information were excluded from the figure, all negative for MOG/AQP4/PLP1-IgG. **The SN-NMOSD group includes 3 PLP1-IgG–positive patients (2 from the exploratory and 1 from the validation cohort) also classified as CNS+PNS-ADD. ADD = autoimmune demyelinating disorder; AQP4 = aquaporin 4; CBA = cell-based assay; CNS+PNS ADD = ADD with peripheral and CNS involvement; MOG = myelin oligodendrocyte glycoprotein; MOGAD = myelin oligodendrocyte glycoprotein antibody–associated disease; MS = multiple sclerosis; n = number; NMOSD = neuromyelitis optica spectrum disorder; ON = optic neuritis; PLP1 = proteolipid protein-1; SN = seronegative; TM = transverse myelitis.

Article Snippet: Live cell-based assays were implemented using the following plasmids: EGFP-tagged full-length human MOG and untagged rat full-length MOG (kind gift of Prof M. Reindl); GFP-tagged human PLP1 (code: RG218616, Origene); untagged human PLP1 (code: SC119823, Origene); untagged human DM20 (a splicing variant of PLP1, code: SC121107, Origene).

Techniques: Biomarker Discovery, Diagnostic Assay, Cell Based Assay

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Conformational Antibodies to Proteolipid Protein-1 and Its Peripheral Isoform DM20 in Patients With CNS Autoimmune Demyelinating Disorders

doi: 10.1212/NXI.0000000000200359

Figure Lengend Snippet: (A) Clinical phenotypes of patients with ADDs split into 3 groups: PLP1-IgG–positive other ADDs, PLP1-IgG–positive MOGAD, and PLP1-IgG–positive MS; (B) EDSS-measured disability over the disease course; (C) median EDSS scores at the last follow-up (or MSSS for patients with MS) in PLP1-IgG–positive patients with MOGAD or with MS, compared with those measured, over the disease course, in the corresponding PLP1-IgG–negative groups. ADD = autoimmune demyelinating disorder; b = bilateral; BsE = brainstem encephalitis; CerS = cerebellar syndrome; CNS+PNS ADD = ADD with peripheral and CNS involvement; EDSS = Expanded Disability Status Scale; FU = follow-up; EMyR = encephalomyeloradiculitis; LETM = longitudinally extensive transverse myelitis; My = myelitis; MyR = myeloradiculitis; MOGAD = myelin oligodendrocyte glycoprotein antibody–associated disease; MS = multiple sclerosis; MSSS = MS severity score; ON = optic neuritis; PLP1 = proteolipid protein-1; TDL = tumefactive demyelinating lesions.

Article Snippet: Live cell-based assays were implemented using the following plasmids: EGFP-tagged full-length human MOG and untagged rat full-length MOG (kind gift of Prof M. Reindl); GFP-tagged human PLP1 (code: RG218616, Origene); untagged human PLP1 (code: SC119823, Origene); untagged human DM20 (a splicing variant of PLP1, code: SC121107, Origene).

Techniques:

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Conformational Antibodies to Proteolipid Protein-1 and Its Peripheral Isoform DM20 in Patients With CNS Autoimmune Demyelinating Disorders

doi: 10.1212/NXI.0000000000200359

Figure Lengend Snippet: Clinical, Laboratory, and Radiologic Features of PLP1-IgG–Positive Patients

Article Snippet: Live cell-based assays were implemented using the following plasmids: EGFP-tagged full-length human MOG and untagged rat full-length MOG (kind gift of Prof M. Reindl); GFP-tagged human PLP1 (code: RG218616, Origene); untagged human PLP1 (code: SC119823, Origene); untagged human DM20 (a splicing variant of PLP1, code: SC121107, Origene).

Techniques:

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Conformational Antibodies to Proteolipid Protein-1 and Its Peripheral Isoform DM20 in Patients With CNS Autoimmune Demyelinating Disorders

doi: 10.1212/NXI.0000000000200359

Figure Lengend Snippet: Clinical, Laboratory, and Radiologic Features of PLP1-IgG–Positive Patients Classified as Other Demyelinating Disorders of the CNS

Article Snippet: Live cell-based assays were implemented using the following plasmids: EGFP-tagged full-length human MOG and untagged rat full-length MOG (kind gift of Prof M. Reindl); GFP-tagged human PLP1 (code: RG218616, Origene); untagged human PLP1 (code: SC119823, Origene); untagged human DM20 (a splicing variant of PLP1, code: SC121107, Origene).

Techniques:

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Conformational Antibodies to Proteolipid Protein-1 and Its Peripheral Isoform DM20 in Patients With CNS Autoimmune Demyelinating Disorders

doi: 10.1212/NXI.0000000000200359

Figure Lengend Snippet: (A) Serum of a patient with PLP1-IgG binds to DM20-transfected CBA (green) and colocalizes with a PLP1 mAb (red) directed against an extracellular loop of the protein (top row). No binding is observed with serum from a HC (bottom row). (B) Binding to both PLP1- and DM20-transfected cells (green) is observed with both PLP1mAb (first row) and serum from a patient with CNS+PNS ADD (pt#1; second row). Conversely, serum from a patient with CNS ADD binds exclusively to PLP1-transfected cells and not to DM20-transfected cells (third row). No binding is observed with serum from a HC (bottom row). DAPI stains the nuclei. (C) Heatmap representing fluorescence intensity of the available samples (n = 36) in either PLP1 or DM20 CBA measured with a semiquantitative score. All PLP1-IgG–positive patients with CNS + PNS ADD, unlike patients with other disease groups, were positive on both PLP1 and DM20 CBA. Scale bars: 10 µm. ADD = autoimmune demyelinating disorder; CBA = cell-based assay; Ig = immunoglobulin; HC = healthy control; mAbs = monoclonal antibodies; PNS = peripheral nervous system; PLP1 = proteolipid protein-1; Pt = patient.

Article Snippet: Live cell-based assays were implemented using the following plasmids: EGFP-tagged full-length human MOG and untagged rat full-length MOG (kind gift of Prof M. Reindl); GFP-tagged human PLP1 (code: RG218616, Origene); untagged human PLP1 (code: SC119823, Origene); untagged human DM20 (a splicing variant of PLP1, code: SC121107, Origene).

Techniques: Transfection, Binding Assay, Fluorescence, Cell Based Assay, Control, Bioprocessing

Journal: Neurology® Neuroimmunology & Neuroinflammation

Article Title: Conformational Antibodies to Proteolipid Protein-1 and Its Peripheral Isoform DM20 in Patients With CNS Autoimmune Demyelinating Disorders

doi: 10.1212/NXI.0000000000200359

Figure Lengend Snippet: (A) Serum and CSF PLP1-IgG titers tested in 41 serum and 24 CSF samples. The red dotted line indicates the cutoff for serum (1:40, left graph) and for CSF (1:5, right graph). (B) Serum:CSF PLP1-IgG titer ratio. The dotted arrow marks the 200:1 ratio expected in physiologic conditions. Values below the dotted line might associate with intrathecal synthesis. Blue dots represent patients positive in serum only, red dots patients positive in CSF only, and black dots patients positive in both serum and CSF. (C) PLP1-IgG subclasses in the whole cohort and in the 3 diagnostic groups of PLP1-IgG–positive patients. (D) PLP-IgG1 complement-dependent cytotoxicity. Data are normalized according to the amount of viable cells found in untreated cells (PLP1 cells, first 2 columns), which is considered 100%. The graph represents the finding after incubation with patient's sera. A reduction of viable cells is detected for 2 AQP4-IgG–positive serum samples (AQP4-01 and AQP4-02), which were used as positive controls, only when incubated with AQP4-transfected cells and in the presence of complement. Similarly, 3 of 8 PLP1-IgG–positive serum samples (other ADD#4, MS#5, and other ADD#18) showed reduced cell viability only when incubated with PLP1-transfected cells in the presence of complement. No effect is observed for the same samples in the absence of complement or after PLP1-IgG preadsorption performed on samples MS#5 and other ADD#18. The black dotted line represents the mean transfection rate for AQP4 and PLP1 (35%), which should theoretically set the limit for the maximum CDC effect in this experimental setting. CDC, which should involve only transfected cells, is unlikely below the cutoff. The numeric codes attributed to PLP1-IgG–positive patients correspond to those of , eTable 2, and eTable 3. ADD = autoimmune demyelinating disorder; AQP4 = aquaporin 4; Ig = immunoglobulin; MOGAD = myelin oligodendrocyte glycoprotein antibody–associated disease; MS = multiple sclerosis; PLP1 = proteolipid protein-1.

Article Snippet: Live cell-based assays were implemented using the following plasmids: EGFP-tagged full-length human MOG and untagged rat full-length MOG (kind gift of Prof M. Reindl); GFP-tagged human PLP1 (code: RG218616, Origene); untagged human PLP1 (code: SC119823, Origene); untagged human DM20 (a splicing variant of PLP1, code: SC121107, Origene).

Techniques: Diagnostic Assay, Incubation, Transfection