pmc07582552 Search Results


labeling check reagent pe  (Miltenyi Biotec)
94
Miltenyi Biotec labeling check reagent pe
Labeling Check Reagent Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmc07582552/Labeling+Check+Reagent/pmc07582552-116-16-26
Average 94 stars, based on 1 article reviews
labeling check reagent pe - by Bioz Stars, 2026-09
94/100 stars
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k562 cell lines  (ATCC)
99
ATCC k562 cell lines
Evaluation of single-cell WGA quality. ( A ) Bioanalyzer profiles of amplified DNA coming from single <t>K562</t> cells fixed with PFA 0.5% for 15 min. Color code: PicoPlex in red, Ampli1 in blue, Malbac in green, RepliG in light blue and GenomePlex in purple. ( B ) Percentage of sequencing efficiency, performed on a region of the ASXL1 gene, obtained with single-cell WGA kits ( n = 20 cells for each kit). ( C ) Histogram representing the rate of ADO effect obtained with the analysis of the heterozygous variant of ASXL1 gene (c.1773C>G) carried by K562 cell line ( n = 20 cells for each kit). ( D ) Representative peaks of homozygous, heterozygous and wild-type alleles identified using Sanger sequencing. Sequences were obtained by different single-cell WGA kits ( n = 20 cells for each kit) and were related to the heterozygous variant of ASXL1 gene (c.1773C>G) carried by K562 cell line.
K562 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmc07582552/K-562/pmc07582552-124-2-9
Average 99 stars, based on 1 article reviews
k562 cell lines - by Bioz Stars, 2026-09
99/100 stars
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repli g single cell kit  (Qiagen)
98
Qiagen repli g single cell kit
Evaluation of single-cell WGA quality. ( A ) Bioanalyzer profiles of amplified DNA coming from single <t>K562</t> cells fixed with PFA 0.5% for 15 min. Color code: PicoPlex in red, Ampli1 in blue, Malbac in green, RepliG in light blue and GenomePlex in purple. ( B ) Percentage of sequencing efficiency, performed on a region of the ASXL1 gene, obtained with single-cell WGA kits ( n = 20 cells for each kit). ( C ) Histogram representing the rate of ADO effect obtained with the analysis of the heterozygous variant of ASXL1 gene (c.1773C>G) carried by K562 cell line ( n = 20 cells for each kit). ( D ) Representative peaks of homozygous, heterozygous and wild-type alleles identified using Sanger sequencing. Sequences were obtained by different single-cell WGA kits ( n = 20 cells for each kit) and were related to the heterozygous variant of ASXL1 gene (c.1773C>G) carried by K562 cell line.
Repli G Single Cell Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmc07582552/REPLI-g+WTA+Single+Cell+Kit/pmc07582552-127-27-31
Average 98 stars, based on 1 article reviews
repli g single cell kit - by Bioz Stars, 2026-09
98/100 stars
  Buy from Supplier

Image Search Results


Evaluation of single-cell WGA quality. ( A ) Bioanalyzer profiles of amplified DNA coming from single K562 cells fixed with PFA 0.5% for 15 min. Color code: PicoPlex in red, Ampli1 in blue, Malbac in green, RepliG in light blue and GenomePlex in purple. ( B ) Percentage of sequencing efficiency, performed on a region of the ASXL1 gene, obtained with single-cell WGA kits ( n = 20 cells for each kit). ( C ) Histogram representing the rate of ADO effect obtained with the analysis of the heterozygous variant of ASXL1 gene (c.1773C>G) carried by K562 cell line ( n = 20 cells for each kit). ( D ) Representative peaks of homozygous, heterozygous and wild-type alleles identified using Sanger sequencing. Sequences were obtained by different single-cell WGA kits ( n = 20 cells for each kit) and were related to the heterozygous variant of ASXL1 gene (c.1773C>G) carried by K562 cell line.

Journal: International Journal of Molecular Sciences

Article Title: Genomic Analysis of Hematopoietic Stem Cell at the Single-Cell Level: Optimization of Cell Fixation and Whole Genome Amplification (WGA) Protocol

doi: 10.3390/ijms21197366

Figure Lengend Snippet: Evaluation of single-cell WGA quality. ( A ) Bioanalyzer profiles of amplified DNA coming from single K562 cells fixed with PFA 0.5% for 15 min. Color code: PicoPlex in red, Ampli1 in blue, Malbac in green, RepliG in light blue and GenomePlex in purple. ( B ) Percentage of sequencing efficiency, performed on a region of the ASXL1 gene, obtained with single-cell WGA kits ( n = 20 cells for each kit). ( C ) Histogram representing the rate of ADO effect obtained with the analysis of the heterozygous variant of ASXL1 gene (c.1773C>G) carried by K562 cell line ( n = 20 cells for each kit). ( D ) Representative peaks of homozygous, heterozygous and wild-type alleles identified using Sanger sequencing. Sequences were obtained by different single-cell WGA kits ( n = 20 cells for each kit) and were related to the heterozygous variant of ASXL1 gene (c.1773C>G) carried by K562 cell line.

Article Snippet: HEL and K562 cell lines were obtained from the ATCC and cultured in RPMI medium (Euroclone S.p.A, Pero, MI, Italy), supplemented with 10% heat-inactivated FBS (Sigma-Aldrich, St. Louis, MO, USA) and 1 mmol/L l-Glutamine (Euroclone) [ ].

Techniques: Amplification, Multiple Annealing and Looping–Based Amplification Cycles, Sequencing, Variant Assay