Journal: bioRxiv

Article Title: TRPA1 channel activation by synthetic lipid nanoparticles

doi: 10.64898/2026.05.03.722497

Figure Lengend Snippet: A Dynamics of relative fluorescence anisotropy measured in CHO-mTRPA1 cells exposed to increasing concentrations of LNP preparations at pH 6.2. B Quantification of the relative fluorescence anisotropy measured in CHO-mTRPA1 and CHO-WT cells at different time points.

Article Snippet: CHO-WT were co-incubated with Pluronic F-127 20% solution in DMSO (1:40 dilution, Biotium) to facilitate cell loading.

Techniques: Fluorescence

Journal: bioRxiv

Article Title: TRPA1 channel activation by synthetic lipid nanoparticles

doi: 10.64898/2026.05.03.722497

Figure Lengend Snippet: A Representative traces of basal activity in CHO-WT cells at pH 6.2. B Representative traces of Ca 2+ transients induced by LNP 10 11 p/mL in CHO-WT cells at pH 6.2. C Dose response of the percentage of cells responding to LNP 10 11 p/mL in CHO-WT cells at pH 6.2. The asterisks correspond to the comparison between each concentration of LNP and the corresponding vehicle solution. D-F Quantification of Ca 2+ responses induced by LNP preparations in the presence of the nonspecific Ca 2+ channel blocker RR in CHO-WT cells at pH 6.2: (D) percentage of responding cells, (E) number of transients per cell and (F) median amplitude of the Ca 2+ transients induced in responding cells. The asterisks correspond to the comparison between each application and LNP 10 11 p/mL.

Article Snippet: CHO-WT were co-incubated with Pluronic F-127 20% solution in DMSO (1:40 dilution, Biotium) to facilitate cell loading.

Techniques: Activity Assay, Comparison, Concentration Assay

Journal: bioRxiv

Article Title: TRPA1 channel activation by synthetic lipid nanoparticles

doi: 10.64898/2026.05.03.722497

Figure Lengend Snippet: Dose response curves of the percentage of (A) number of Ca 2+ transients and (B) median amplitude of Ca 2+ transients in responding CHO-WT cells at pH 6.2. The asterisks correspond to the comparison between each concentration of LNP and the corresponding vehicle solution.

Article Snippet: CHO-WT were co-incubated with Pluronic F-127 20% solution in DMSO (1:40 dilution, Biotium) to facilitate cell loading.

Techniques: Comparison, Concentration Assay

Journal: bioRxiv

Article Title: TRPA1 channel activation by synthetic lipid nanoparticles

doi: 10.64898/2026.05.03.722497

Figure Lengend Snippet: A-C Representative traces of Ca 2+ responses induced by LNP 10 11 p/mL in the absence of extracellular Ca 2+ in (A) CHO-mTRPA1 cells, (B) CHO-WT cells, and (C) DRG neurons, at pH 6.2. D-F Quantification of Ca 2+ responses induced by 10 11 p/mL in the absence of extracellular Ca 2+ in CHO-mTRPA1 cells, CHO-WT cells, and DRG neurons, at pH 6.2: (D) percentage of responding cells, (E) number of transients and (F) median amplitude of the Ca 2+ transients induced in responding cells.

Article Snippet: CHO-WT were co-incubated with Pluronic F-127 20% solution in DMSO (1:40 dilution, Biotium) to facilitate cell loading.

Techniques:

Journal: bioRxiv

Article Title: TRPA1 channel activation by synthetic lipid nanoparticles

doi: 10.64898/2026.05.03.722497

Figure Lengend Snippet: LNP preparations trigger Ca 2+ mobilization from the ER. A Representative traces of ER luminal [Ca 2+ ] in CHO-mTRPA1 cells at pH 6.2 upon injection of LNP 10 11 p/mL. B Representative traces of ER luminal [Ca 2+ ] in CHO-WT cells at pH 6.2 upon injection of LNP 10 11 p/mL. C-E Quantification of the luminal Ca 2+ release upon LNP application: (C) percentage of responding cells, (D) number of transients per responding cell, (E) median relative amplitude of the transients in responding cells. The asterisks correspond to the comparison between LNP 10 11 p/mL and Krebs, for each cell line. F-H Quantification of the LNP-induced cytosolic Ca 2+ transients upon ER emptying with thapsigargin: (F) percentage of responding cells, (G) number of transients per responding cell, (H) median relative amplitude of the transients in responding cells. The asterisks correspond to the comparison between each application and LNP 10 11 p/mL + TG.

Article Snippet: CHO-WT were co-incubated with Pluronic F-127 20% solution in DMSO (1:40 dilution, Biotium) to facilitate cell loading.

Techniques: Injection, Comparison

Journal: bioRxiv

Article Title: TRPA1 channel activation by synthetic lipid nanoparticles

doi: 10.64898/2026.05.03.722497

Figure Lengend Snippet: Representative traces of Ca 2+ responses induced by LNP 10 11 p/mL upon ER depletion with thapsigargin in (A) CHO-mTRPA1 cells and (B) CHO-WT cells, at pH 6.2.

Article Snippet: CHO-WT were co-incubated with Pluronic F-127 20% solution in DMSO (1:40 dilution, Biotium) to facilitate cell loading.

Techniques:

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Preparation and in vitro sustained release performance of thermosensitive gel microsphere composite loaded with Anti-Tuberculosis drug PaMZ/BMP-2

doi: 10.1186/s13018-025-06595-1

Figure Lengend Snippet: The Relationship between P407/P188 ratio and temperature

Article Snippet: Briefly, mixed solutions of P407 and P188 (HY-D1005A and HY-D1005, MedChemExpress) with varying weight ratios and total concentrations ranging from 15 to 30% (w/v) were prepared in 1 mL of 0.9% normal saline.

Techniques: