Journal: Hospital Pharmacy

Article Title: Approvals, Submission, and Important Labeling Changes for US Marketed Pharmaceuticals

doi: 10.1310/hpj4808-682

Figure Lengend Snippet: Agents pending FDA approval: May 18, 2013 through July 17, 2013

Article Snippet: Elosulfase alfa , Vimizim (BioMarin Pharmaceutical) , Treatment of patients suffering from mucopolysaccharidosis type IVA (MPS IVA) or morquio A syndrome. Priority review, but the date on which the agency will announce drug approval status was delayed by 3 months at the end of May 2013..

Techniques: Injection, Formulation

Journal: Peptides

Article Title: An angiotensin-converting enzyme-2-derived heptapeptide GK-7 for SARS-CoV-2 spike blockade

doi: 10.1016/j.peptides.2021.170638

Figure Lengend Snippet: Diagram depicting the design of short peptides derived from ACE2. The extracellular domain of ACE2 is presented as ribbon structure. EQ-10, KT-11, LD-11, NH-11, and ED-10 are derived from the N-terminal helix of ACE2. GK-7 is extracted from the linker between β3 and β4 of ACE2. The critical residues contributing to the interaction of ACE2 with SARS-CoV-2 spike in the peptides are shown in sticks and colored red.

Article Snippet: SARS-CoV-2 spike pseudovirions (20 μL, 1.98 × 10 7 TU/mL) preincubated with increasing concentrations of GK-7 (0.39, 0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 75, and 100 μg/mL), LD-11 (0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 75, 100, and 200 μg/mL), or ACE2 (10108-H02H, Sino Biological, 0.005, 0.01, 0.02, 0.04, 0.08, 0.16, 0.31, 0.63, and 1.2 μg/mL) at 37 °C for 1 h were added to the cells, followed by 12 h of coincubation.

Techniques: Derivative Assay

Journal: Peptides

Article Title: An angiotensin-converting enzyme-2-derived heptapeptide GK-7 for SARS-CoV-2 spike blockade

doi: 10.1016/j.peptides.2021.170638

Figure Lengend Snippet: Inhibitory effects of GK-7 on SARS-CoV-2 S1 adherence to cells. ( A ) Western blotting detection of SARS-CoV-2 S1 binding to A549 cells in the absence and presence of 50 μg/mL LD-11, ED-10, and GK-7. β-actin was used as the reference. ( B ) Dose-dependent suppression of GK-7 on SARS-CoV-2 S1 binding to A549 cells. ( C ) Immunofluorescence microscopy revealing the protective effect of GK-7 on A549 cells exposed to SARS-CoV-2 S1 (red). Nuclei were stained with DAPI (blue). The scale bar indicates 50 μm.

Article Snippet: SARS-CoV-2 spike pseudovirions (20 μL, 1.98 × 10 7 TU/mL) preincubated with increasing concentrations of GK-7 (0.39, 0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 75, and 100 μg/mL), LD-11 (0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 75, 100, and 200 μg/mL), or ACE2 (10108-H02H, Sino Biological, 0.005, 0.01, 0.02, 0.04, 0.08, 0.16, 0.31, 0.63, and 1.2 μg/mL) at 37 °C for 1 h were added to the cells, followed by 12 h of coincubation.

Techniques: Western Blot, Binding Assay, Immunofluorescence, Microscopy, Staining

Journal: Peptides

Article Title: An angiotensin-converting enzyme-2-derived heptapeptide GK-7 for SARS-CoV-2 spike blockade

doi: 10.1016/j.peptides.2021.170638

Figure Lengend Snippet: Antiviral action of GK-7 against SARS-CoV-2 spike pseudovirion infection. ( A ) Dose-dependent antiviral activity of GK-7, ( B ) LD-11, and ( C ) ACE2 against pseudovirions. Results are shown as mean ± standard deviation (SD). The fitted curves obtained by nonlinear regression are in burgundy.

Article Snippet: SARS-CoV-2 spike pseudovirions (20 μL, 1.98 × 10 7 TU/mL) preincubated with increasing concentrations of GK-7 (0.39, 0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 75, and 100 μg/mL), LD-11 (0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 75, 100, and 200 μg/mL), or ACE2 (10108-H02H, Sino Biological, 0.005, 0.01, 0.02, 0.04, 0.08, 0.16, 0.31, 0.63, and 1.2 μg/mL) at 37 °C for 1 h were added to the cells, followed by 12 h of coincubation.

Techniques: Infection, Activity Assay, Standard Deviation

Journal: Journal of Clinical Microbiology

Article Title: “Pathotyping” Multiplex PCR Assay for Haemophilus parasuis : a Tool for Prediction of Virulence

doi: 10.1128/JCM.02464-16

Figure Lengend Snippet: Results of the pathotyping multiplex on a subset of H. parasuis isolates from the additional isolate collection. M, Quick-load 100 bp DNA ladder.

Article Snippet: Gel electrophoresis was performed using 2.0% agarose gel with 5% SybrSafe dye (Invitrogen) and run at 110 V for 90 min using the Quick-load 100 bp DNA ladder (New England BioLabs) for all mPCRs.

Techniques: Multiplex Assay

Journal: Journal of Clinical Microbiology

Article Title: “Pathotyping” Multiplex PCR Assay for Haemophilus parasuis : a Tool for Prediction of Virulence

doi: 10.1128/JCM.02464-16

Figure Lengend Snippet: Negative-control panel showing specificity of the primer sets across a range of commensal and pathogenic bacteria of the pig respiratory tract (Haemophilus parasuis positive controls serovar 2 (SW140) and serovar 5 (Nagasaki), Actinobacillus minor, A. porcinus, A. indolicus, Streptococcus suis, A. pleuropneumoniae, and Bordetella bronchiseptica). M, Quick-load 100 bp DNA ladder.

Article Snippet: Gel electrophoresis was performed using 2.0% agarose gel with 5% SybrSafe dye (Invitrogen) and run at 110 V for 90 min using the Quick-load 100 bp DNA ladder (New England BioLabs) for all mPCRs.

Techniques: Negative Control

Journal: Journal of Clinical Microbiology

Article Title: “Pathotyping” Multiplex PCR Assay for Haemophilus parasuis : a Tool for Prediction of Virulence

doi: 10.1128/JCM.02464-16

Figure Lengend Snippet: Limit of detection for the pathotyping multiplex PCR using pure genomic DNA (genomes/μl) for seven isolates representing all of the bands present in the pathotyping multiplex PCR. M, Quick-load 100 bp DNA ladder.

Article Snippet: Gel electrophoresis was performed using 2.0% agarose gel with 5% SybrSafe dye (Invitrogen) and run at 110 V for 90 min using the Quick-load 100 bp DNA ladder (New England BioLabs) for all mPCRs.

Techniques: Multiplex Assay