Journal: Scientific Reports

Article Title: Plin2-deficiency reduces lipophagy and results in increased lipid accumulation in the heart

doi: 10.1038/s41598-019-43335-y

Figure Lengend Snippet: Elevated Plin3 and Plin5 protein expression in Plin2 −/− hearts. ( A ) Representative figure of immunoblot analysis of Plin3 and Plin5 using protein lysates from Plin2 +/+ and Plin2 −/− hearts. Full-length blots are presented in Supplementary Fig. . ( B ) Quantification of immunoblot analysis on Plin3 and Plin5 (n = 8–9). Data are presented as mean ± SEM, * p < 0.05 vs. Plin2 +/+ and **p < 0.01 vs. Plin2 +/+ . ( C , D ) Representative confocal microscopy images of Plin2 +/+ and Plin2 −/− cardiomyocytes. Images are shown as a max stack containing 10 z-stacks compressed into one single image and a higher magnification z-stack image. ( C ) Plin3 is shown in green, ORO (LDs) in red, nuclei in blue and Plin3 and lipid droplet co-localization in yellow. ( D ) Plin5 is shown in green, LDs in red, nuclei in blue. Co-localization of Plin5 and LD is indicated with arrows, whereas Plin5-positive staining without association to LDs are shown with arrow heads (x630 magnification, scale bar 10 µm, higher magnification image scale bar 2, 5 µm).

Article Snippet: The following TaqMan Gene Expression assays were used: Plin2 Mm00558672_m1; Plin3 Mm04208646_g1; Plin4 Mm00491061_m1; Plin5 Mm00508852_m1; and HPRT Mm01545399_m1 as an internal control.

Techniques: Expressing, Western Blot, Confocal Microscopy, Staining

Journal: Frontiers in oncology

Article Title: Proteomic Characterization of Cytoplasmic Lipid Droplets in Human Metastatic Breast Cancer Cells.

doi: 10.3389/fonc.2021.576326

Figure Lengend Snippet: FIGURE 7 | SQLE and NSDHL localize to cytoplasmic lipid droplets (CLDs) in MCF10CA1a cells. Representative immunofluorescence images of MCF10CA1a cells. Cells were stained with Alexa Fluor 633 to visualize SQLE (A) and NSDHL (B), BODIPY to visualize CLDs, and DAPI to visualize nuclei. Signals from all three channels were merged for the final image in (A, B).

Article Snippet: Cells were probed with antibodies for PLIN3, SQLE, and NSDHL (Sigma, HPA006427; SantaCruz Biotechnologies, sc-271651; Atlas Antibodies, HPA000571, respectively).

Techniques: Staining

Journal: Cell reports

Article Title: ATGL/CGI-58-Dependent Hydrolysis of a Lipid Storage Pool in Murine Enterocytes

doi: 10.1016/j.celrep.2019.07.030

Figure Lengend Snippet: (A) Radioactivity in the SI and liver of chow diet-fed WT and iDKO mice (n = 4 or 5) 30 min post-gavage of 2 μCi [9,10- 3 H(N)]-triolein in 200 μL corn oil. (B) ORO staining of duodenal sections 30 min after an oral lipid load. (C and D) Biochemical (C) and histological (D) analysis of hepatic lipid levels 30 min post-gavage of 200 μL corn oil. (E and F) Lipid concentrations (E) and (F) lipoprotein profiles in the plasma 30 min post-gavage of corn oil (200 μL). Data represent mean + SD (n = 3 or 4). *p < 0.05, **p ≤ 0.01, and ***p ≤ 0.001. Magnification, 40×; scale bar, 50 μm. (G and H) Chow diet-fed mice were fasted for 16 h prior to an oral administration of 100 μL corn oil containing 1 μg/g body weight BODIPY FL C 16 (green). Intestinal sections were co-stained with PLIN3 (red) to visualize colocalization with cLDs. PLIN3 immunofluorescence staining 30 min (G) and 2 h (H) post-gavage. Green background fluorescence results from high chlorophyll content in the chow diet (alfalfa). Arrows indicate cLDs originating from BODIPY-labeled FA, which colocalize with PLIN3; arrowheads indicate endogenous cLDs coated with PLIN3; stars indicate BODIPY-containing cLDs, which do not colocalize with PLIN3. Magnification, 100×; scale bar, 10 μm. CE, cholesteryl esters; Duo, duodenum; FC, free cholesterol; HDL, high-density lipoprotein; Hep, hepar (liver); Ile, ileum; Jej, jejunum; LDL, low-density lipoprotein; PLIN3, Perilipin 3; Sto, stomach; TC, total cholesterol; TG, triglycerides; VLDL, very low density lipoprotein. See also .

Article Snippet: Sections were incubated overnight at 4°C with the primary antibody against PLIN3 (abs482; 1:200; Merck KGaA, Darmstadt, Germany), followed by incubation with the corresponding Alexa Fluor® 594-labeled secondary antibody for PLIN3 (A11037; 1:250; Thermo Fisher Scientific, Waltham, MA).

Techniques: Radioactivity, Staining, Clinical Proteomics, Immunofluorescence, Fluorescence, Labeling

Journal: Cell reports

Article Title: ATGL/CGI-58-Dependent Hydrolysis of a Lipid Storage Pool in Murine Enterocytes

doi: 10.1016/j.celrep.2019.07.030

Figure Lengend Snippet:

Article Snippet: Sections were incubated overnight at 4°C with the primary antibody against PLIN3 (abs482; 1:200; Merck KGaA, Darmstadt, Germany), followed by incubation with the corresponding Alexa Fluor® 594-labeled secondary antibody for PLIN3 (A11037; 1:250; Thermo Fisher Scientific, Waltham, MA).

Techniques: Diagnostic Assay, Software, Imaging

Journal: Disease Markers

Article Title: Associations of Perilipin 3 with Insulin Resistance in Arab Adults with Type 2 Diabetes

doi: 10.1155/2021/4791915

Figure Lengend Snippet: Clinical characteristics of subjects according to groups.

Article Snippet: A human PLIN3 CLIA kit (Catalogue No. abx494487, Abbexa Ltd., Cambridge, UK) was used to determine PLIN3 in serum through an enzyme-linked immunosorbent assay (ELISA).

Techniques:

Journal: Disease Markers

Article Title: Associations of Perilipin 3 with Insulin Resistance in Arab Adults with Type 2 Diabetes

doi: 10.1155/2021/4791915

Figure Lengend Snippet: PLIN3 levels in the three groups expressed in terms of median, 1 st quartile (gray), and 3 rd quartile (yellow).

Article Snippet: A human PLIN3 CLIA kit (Catalogue No. abx494487, Abbexa Ltd., Cambridge, UK) was used to determine PLIN3 in serum through an enzyme-linked immunosorbent assay (ELISA).

Techniques:

Journal: Disease Markers

Article Title: Associations of Perilipin 3 with Insulin Resistance in Arab Adults with Type 2 Diabetes

doi: 10.1155/2021/4791915

Figure Lengend Snippet: Associations of PLIN3 with studied parameters according to groups.

Article Snippet: A human PLIN3 CLIA kit (Catalogue No. abx494487, Abbexa Ltd., Cambridge, UK) was used to determine PLIN3 in serum through an enzyme-linked immunosorbent assay (ELISA).

Techniques:

Journal: Disease Markers

Article Title: Associations of Perilipin 3 with Insulin Resistance in Arab Adults with Type 2 Diabetes

doi: 10.1155/2021/4791915

Figure Lengend Snippet: Significant associations of PLIN3 with (a) BMI, (b) glucose, (c) insulin, and (d) HOMA-IR.

Article Snippet: A human PLIN3 CLIA kit (Catalogue No. abx494487, Abbexa Ltd., Cambridge, UK) was used to determine PLIN3 in serum through an enzyme-linked immunosorbent assay (ELISA).

Techniques:

Journal: Disease Markers

Article Title: Associations of Perilipin 3 with Insulin Resistance in Arab Adults with Type 2 Diabetes

doi: 10.1155/2021/4791915

Figure Lengend Snippet: Independent predictors of PLIN3.

Article Snippet: A human PLIN3 CLIA kit (Catalogue No. abx494487, Abbexa Ltd., Cambridge, UK) was used to determine PLIN3 in serum through an enzyme-linked immunosorbent assay (ELISA).

Techniques:

Journal: Disease Markers

Article Title: Associations of Perilipin 3 with Insulin Resistance in Arab Adults with Type 2 Diabetes

doi: 10.1155/2021/4791915

Figure Lengend Snippet: Clinical characteristics of males and females according to studied groups.

Article Snippet: A human PLIN3 CLIA kit (Catalogue No. abx494487, Abbexa Ltd., Cambridge, UK) was used to determine PLIN3 in serum through an enzyme-linked immunosorbent assay (ELISA).

Techniques: