plif Search Results


plasmid encoding ferritin heavy chain 1  (OriGene)
90
OriGene plasmid encoding ferritin heavy chain 1
Plasmid Encoding Ferritin Heavy Chain 1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plif/Ferritin+Heavy+Chain+(FTH1)+(NM_002032)+Human+Tagged+ORF+Clone/pmc06459820-181-5-12
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fth1 cdna plasmid  (OriGene)
90
OriGene fth1 cdna plasmid
Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through <t>cDNA</t> plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.
Fth1 Cdna Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plif/Ferritin+Heavy+Chain+(FTH1)+(NM_002032)+Human+Tagged+ORF+Clone/pm35903602-60-0-4
Average 90 stars, based on 1 article reviews
fth1 cdna plasmid - by Bioz Stars, 2026-10
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antiferritin heavy chain  (Proteintech)
94
Proteintech antiferritin heavy chain
Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through <t>cDNA</t> plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.
Antiferritin Heavy Chain, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plif/FTH1+Fusion+Protein/10__1021_slash_envhealth__5c00677-94-7-12
Average 94 stars, based on 1 article reviews
antiferritin heavy chain - by Bioz Stars, 2026-10
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ferritin heavy chain fthi antibody  (Boster Bio)
93
Boster Bio ferritin heavy chain fthi antibody
Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through <t>cDNA</t> plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.
Ferritin Heavy Chain Fthi Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plif/Human+Ferritin%2FFTH1+Recombinant+Protein/pm38901284-57-1-9
Average 93 stars, based on 1 article reviews
ferritin heavy chain fthi antibody - by Bioz Stars, 2026-10
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harmonytm plif instrument system  (Spinal Concepts Inc)
90
Spinal Concepts Inc harmonytm plif instrument system
Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through <t>cDNA</t> plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.
Harmonytm Plif Instrument System, supplied by Spinal Concepts Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plif/harmonytm+plif+instrument+system/us08419653-781-17-23
Average 90 stars, based on 1 article reviews
harmonytm plif instrument system - by Bioz Stars, 2026-10
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plif-ko-flag  (Genlantis inc)
90
Genlantis inc plif-ko-flag
Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through <t>cDNA</t> plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.
Plif Ko Flag, supplied by Genlantis inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plif/plif+ko+flag/pm32002841-77-3-13
Average 90 stars, based on 1 article reviews
plif-ko-flag - by Bioz Stars, 2026-10
90/100 stars
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nh plif field  (Markstein Sichtec Medical)
90
Markstein Sichtec Medical nh plif field
Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through <t>cDNA</t> plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.
Nh Plif Field, supplied by Markstein Sichtec Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plif/nh+plif+field/pmc08154360-252-18-8
Average 90 stars, based on 1 article reviews
nh plif field - by Bioz Stars, 2026-10
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plif cages plivios  (DePuy Synthes)
90
DePuy Synthes plif cages plivios
Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through <t>cDNA</t> plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.
Plif Cages Plivios, supplied by DePuy Synthes, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plif/plif+cages+plivios/pmc08027195-75-1-5
Average 90 stars, based on 1 article reviews
plif cages plivios - by Bioz Stars, 2026-10
90/100 stars
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plif technique  (COMSOL Inc)
90
COMSOL Inc plif technique
Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through <t>cDNA</t> plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.
Plif Technique, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plif/plif+technique/10__1002_slash_aic__13805-38-11-20
Average 90 stars, based on 1 article reviews
plif technique - by Bioz Stars, 2026-10
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plif-wt-flag  (Genlantis inc)
90
Genlantis inc plif-wt-flag
Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through <t>cDNA</t> plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.
Plif Wt Flag, supplied by Genlantis inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plif/plif+wt+flag/pm32002841-77-1-13
Average 90 stars, based on 1 article reviews
plif-wt-flag - by Bioz Stars, 2026-10
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mas plif system screws  (NuVasive Inc)
90
NuVasive Inc mas plif system screws
Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through <t>cDNA</t> plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.
Mas Plif System Screws, supplied by NuVasive Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plif/mas+plif+system+screws/pmc07595813-61-9-21
Average 90 stars, based on 1 article reviews
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plif images  (MedWell Foods)
90
MedWell Foods plif images
Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through <t>cDNA</t> plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.
Plif Images, supplied by MedWell Foods, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plif/plif+images/10__3389_slash_fmech__2019__00065-413-2-21
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plif images - by Bioz Stars, 2026-10
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Image Search Results


Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through cDNA plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.

Journal: Journal of clinical biochemistry and nutrition

Article Title: Intracellular ferritin heavy chain plays the key role in artesunate-induced ferroptosis in ovarian serous carcinoma cells.

doi: 10.3164/jcbn.21-82

Figure Lengend Snippet: Fig. 4. Effect of FTH gene modulation on ART-induced cell death. (A) Profile of FTH level by Western blotting analysis in CaOV3 and SKOV3ip1 cells and FTH silenced in SKOV3ip1 cells by siRNA transfection. (B) Effect of FTH silencing on ART-induced reduction of cell viability in CaOV3 and SKOV3ip1 cells after 24 h of treatment. (C, D) Effect of FTH silencing on ART (70 μM)-enhanced lipid peroxidation in SKOV3ip1 cells after 24 h of treatment. C represents profile of BODIPY 581/591 C11. n = 7. (E, F) Effect of FTH silencing on ART (70 μM)-induced intracellular Fe2+ in SKOV3ip1 cells after 5 h of treatment. E represents profile of Lyso-RhoNox. n = 7. (G) Western blotting analysis showed that FTH was overexpressed in CaOV3 cells through cDNA plasmid transfection. (H) Effect of FTH overexpression on ART-induced reduction of cell viability in CaOV3 after 24 h of treatment. n = 5. (I, J) Effect of FTH overexpression on ART (70 μM)-enhanced lipid peroxidation in CaOV3 cells after 24 h of treatment. I represents profile of BODIPY 581/591 C11. n = 7. (K, L) Effect of FTH overexpression on ART (70 μM)-induced intracellular Fe2+ in CaOV3 cells after 5 h of treat‐ ment. K represents profile of Lyso-RhoNox. n = 7. DMSO was used as vehicle and scrambled siRNA and empty vector (EV) were used as control. FTH cDNA represents FTH overexpression. *p<0.05.

Article Snippet: FTH1 cDNA plasmid (RC209845; OriGene, Rockville, MD) was used for gene overexpression. pCMV6-Entry Mammalian Expression Vector [empty vector (EV), PS100001; OriGene] were used for negative control.

Techniques: Western Blot, Transfection, Plasmid Preparation, Over Expression, Control