plenti6.3 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher plenti6 3 to v5
    Plenti6 3 To V5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 to v5/product/Thermo Fisher
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 to v5 - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    96
    Thermo Fisher plenti6 3
    Plenti6 3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3/product/Thermo Fisher
    Average 96 stars, based on 151 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    96
    Thermo Fisher plenti6 3 dest
    Plenti6 3 Dest, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 dest/product/Thermo Fisher
    Average 96 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 dest - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    91
    Thermo Fisher plenti6 3 mcs
    Plenti6 3 Mcs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 mcs/product/Thermo Fisher
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 mcs - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    97
    Thermo Fisher plenti6 3 v5 dest
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 V5 Dest, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 257 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 v5 dest/product/Thermo Fisher
    Average 97 stars, based on 257 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 v5 dest - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    85
    Thermo Fisher plenti6 3 gfp
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 Gfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 gfp/product/Thermo Fisher
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 gfp - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    95
    Thermo Fisher plenti6 3 vector
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 vector/product/Thermo Fisher
    Average 95 stars, based on 136 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 vector - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    93
    Genechem plenti6 3 v5 dest
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 V5 Dest, supplied by Genechem, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 v5 dest/product/Genechem
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 v5 dest - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    88
    GenePharma Company plenti6 3 v5 dest
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 V5 Dest, supplied by GenePharma Company, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 v5 dest/product/GenePharma Company
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 v5 dest - by Bioz Stars, 2020-04
    88/100 stars
      Buy from Supplier

    95
    Expression Systems Inc plenti6 3
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3, supplied by Expression Systems Inc, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3/product/Expression Systems Inc
    Average 95 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    95
    Shanghai Genechem plenti6 3 vector
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 Vector, supplied by Shanghai Genechem, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 vector/product/Shanghai Genechem
    Average 95 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 vector - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    92
    Thermo Fisher plenti6 3 v5 vector
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 V5 Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 v5 vector/product/Thermo Fisher
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 v5 vector - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    86
    Thermo Fisher plenti6 3 dest plasmid
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 Dest Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 dest plasmid/product/Thermo Fisher
    Average 86 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 dest plasmid - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    96
    Thermo Fisher plenti6 3 v5 topo
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 V5 Topo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 v5 topo/product/Thermo Fisher
    Average 96 stars, based on 39 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 v5 topo - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    86
    Thermo Fisher plenti6 3 expression vectors
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 Expression Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 expression vectors/product/Thermo Fisher
    Average 86 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 expression vectors - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    91
    GenePharma Company plenti6 3 v5edest vector
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 V5edest Vector, supplied by GenePharma Company, used in various techniques. Bioz Stars score: 91/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 v5edest vector/product/GenePharma Company
    Average 91 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 v5edest vector - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    87
    Thermo Fisher plenti6 3 mcs v5 dest
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 Mcs V5 Dest, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 mcs v5 dest/product/Thermo Fisher
    Average 87 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 mcs v5 dest - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    94
    Thermo Fisher plenti6 3 to v5 dest
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 To V5 Dest, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 to v5 dest/product/Thermo Fisher
    Average 94 stars, based on 98 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 to v5 dest - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    86
    Thermo Fisher plenti6 3 v5 m est
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 V5 M Est, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 v5 m est/product/Thermo Fisher
    Average 86 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 v5 m est - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    96
    Thermo Fisher plenti6 3 lentiviral vector
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 Lentiviral Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 lentiviral vector/product/Thermo Fisher
    Average 96 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 lentiviral vector - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    91
    Addgene inc plenti6 3 to v5 dest
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 To V5 Dest, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 to v5 dest/product/Addgene inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 to v5 dest - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    94
    Addgene inc plenti6 3 hmanfsigpep strepii flag ple
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 Hmanfsigpep Strepii Flag Ple, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 hmanfsigpep strepii flag ple/product/Addgene inc
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 hmanfsigpep strepii flag ple - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    86
    Thermo Fisher plenti6 3⁄v5 dest vector
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3⁄V5 Dest Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3⁄v5 dest vector/product/Thermo Fisher
    Average 86 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    plenti6 3⁄v5 dest vector - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    91
    Thermo Fisher plenti6 3 mcs shuttle vector
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 Mcs Shuttle Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 mcs shuttle vector/product/Thermo Fisher
    Average 91 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 mcs shuttle vector - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    86
    Thermo Fisher plenti6 3 v5 lentiviral vector
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 V5 Lentiviral Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 v5 lentiviral vector/product/Thermo Fisher
    Average 86 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 v5 lentiviral vector - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    87
    Thermo Fisher plenti6 3 topo vector
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 topo vector/product/Thermo Fisher
    Average 87 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 topo vector - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    88
    Thermo Fisher plenti6 3 v5 topo system
    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with <t>pLenti6.3/V5-DEST</t> empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p
    Plenti6 3 V5 Topo System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 v5 topo system/product/Thermo Fisher
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 v5 topo system - by Bioz Stars, 2020-04
    88/100 stars
      Buy from Supplier

    93
    Thermo Fisher plenti6 3 v5 dest plasmid
    p300 is involved in the ability of Cdk9 to phosphorylate RNA pol II and bind with GATA4. A , COS7 cells were co-transfected with 6 μg of pCMVwtp300, 4 μg of pcDNA3. <t>2/V5-DEST-HA-p300-(1514–1922),</t> 1 μg of pcDNA3.2/V5-DEST-FLAG-GATA4,
    Plenti6 3 V5 Dest Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 v5 dest plasmid/product/Thermo Fisher
    Average 93 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 v5 dest plasmid - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    87
    Thermo Fisher plenti6 3 v5 expression plasmid
    p300 is involved in the ability of Cdk9 to phosphorylate RNA pol II and bind with GATA4. A , COS7 cells were co-transfected with 6 μg of pCMVwtp300, 4 μg of pcDNA3. <t>2/V5-DEST-HA-p300-(1514–1922),</t> 1 μg of pcDNA3.2/V5-DEST-FLAG-GATA4,
    Plenti6 3 V5 Expression Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 v5 expression plasmid/product/Thermo Fisher
    Average 87 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 v5 expression plasmid - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    95
    Thermo Fisher plenti6 3 lentiviral vectors
    p300 is involved in the ability of Cdk9 to phosphorylate RNA pol II and bind with GATA4. A , COS7 cells were co-transfected with 6 μg of pCMVwtp300, 4 μg of pcDNA3. <t>2/V5-DEST-HA-p300-(1514–1922),</t> 1 μg of pcDNA3.2/V5-DEST-FLAG-GATA4,
    Plenti6 3 Lentiviral Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 lentiviral vectors/product/Thermo Fisher
    Average 95 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 lentiviral vectors - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    92
    Thermo Fisher plenti6 3 v5 topo vector
    Schematic diagram of construction of IL-10/Lentivirus. IL-10/lentivirus-based plasmid was made by inserting the mIL-10 cDNA into the <t>pLenti6.3/V5-TOPO</t> vector.
    Plenti6 3 V5 Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plenti6 3 v5 topo vector/product/Thermo Fisher
    Average 92 stars, based on 80 article reviews
    Price from $9.99 to $1999.99
    plenti6 3 v5 topo vector - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    Image Search Results


    Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with pLenti6.3/V5-DEST empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p

    Journal: BMC Cancer

    Article Title: Downregulation of SAV1 plays a role in pathogenesis of high-grade clear cell renal cell carcinoma

    doi: 10.1186/1471-2407-11-523

    Figure Lengend Snippet: Downregulation of SAV1 activates YAP1 . a Phosphorylation of YAP1 was decreased in RCC cell lines. Phosphorylation of YAP1 in HK2, 786-O and 769-P cells was analyzed by Western blot analysis with anti-phospho-YAP antibody, anti-YAP antibody, and anti-GAPDH antibody, respectively. Protein level of SAV1 in each cell line was demonstrated with anti-SAV1 antibody. SAV1 was detectable only in HK2 cells. b Phosphorylated YAP1 is increased in SAV1-transduced 786-O cells. 786-O cells were transduced with pLenti6.3/V5-DEST empty vector or SAV1-pLenti6.3/V5-DEST, and one clone of control cells (designated as the control) and two independent clones of SAV1-re-expressing cells (designated as SAV1-1 and SAV1-2) were established. Western blotting was performed with anti-phospho-YAP antibody, anti-YAP antibody, anti-SAV1 antibody and anti-GAPDH antibody, respectively. c SAV1 suppresses transcriptional activity of YAP1-TEAD3. SAV1-1, SAV1-2 and control cells were transfected with the Gal4-TEAD3 plasmid vector that expresses TEAD3 fused to GAL4 (kindly provided by Dr. B. Zhao [ 19 ]), Gal4-9x UAS luciferase reporter (pGL4.31), and pRL-CMV. Firefly luciferase activity was normalized to Renilla luciferase activity. Normalized luciferase activity in control cells untransfected with TEAD3 was set at 1. In SAV1-1 and SAV1-2 cells, the luciferase activity was significantly decreased. Experiments were performed in triplicate. The dual luciferase data are shown as mean ± SD. * p

    Article Snippet: To identify stable clones of SAV1-re-expressing cells, the full-length SAV1 cDNA was cloned into pLenti6.3/V5-DEST (Invitrogen).

    Techniques: Western Blot, Transduction, Plasmid Preparation, Clone Assay, Expressing, Activity Assay, Transfection, Luciferase

    p300 is involved in the ability of Cdk9 to phosphorylate RNA pol II and bind with GATA4. A , COS7 cells were co-transfected with 6 μg of pCMVwtp300, 4 μg of pcDNA3. 2/V5-DEST-HA-p300-(1514–1922), 1 μg of pcDNA3.2/V5-DEST-FLAG-GATA4,

    Journal: The Journal of Biological Chemistry

    Article Title: Cyclin-dependent Kinase-9 Is a Component of the p300/GATA4 Complex Required for Phenylephrine-induced Hypertrophy in Cardiomyocytes *

    doi: 10.1074/jbc.M109.070458

    Figure Lengend Snippet: p300 is involved in the ability of Cdk9 to phosphorylate RNA pol II and bind with GATA4. A , COS7 cells were co-transfected with 6 μg of pCMVwtp300, 4 μg of pcDNA3. 2/V5-DEST-HA-p300-(1514–1922), 1 μg of pcDNA3.2/V5-DEST-FLAG-GATA4,

    Article Snippet: The complementary DNA oligos were annealed and ligated to the entry vectors and subcloned into the pLenti6.3/V5-DEST vector (Invitrogen).

    Techniques: Transfection

    Chronic hypoxia‐induced slug activates expression of ephrin‐B1 through E‐box motifs. A, Relative expression of ephrin‐B1 mRNA in non‐transfected (control), slug si RNA ‐transfected (siSlug) and control si RNA ‐transfected (siScr) LNC aP/ CH 6M cells. B, Relative expression of ephrin‐B1 mRNA in the LNC aP/N cells transfected with various amounts of the slug‐expression vector, pL enti6.3/V5‐ DEST ‐slug (0‐1000 ng). C, Schematic diagram of luciferase reporter constructs containing wild‐type human ephrin‐B1 promoter ( pGL ‐Basic‐ephrin‐B1 promoter WT ), E‐box1 (E1m) or E‐box2 (E2m) mutations, or double mutations (E1mE2m). Arrows, primer pairs flanking E‐box motifs used for chromatin immunoprecipitation (ChIP) analysis. D, Effect of slug overexpression on the wild‐type ephrin‐B1 promoter activity. LNC aP/N cells were transfected with 1000 ng pGL ‐Basic‐ephrin‐B1 promoter WT and various amounts of the slug‐expression vector, pL enti6.3/V5‐ DEST ‐slug (0‐1000 ng). Firefly luciferase activity was normalized to Renilla luciferase activity. E, Mutational analysis of the E‐box motifs in the ephrin‐B1 promoter. LNC aP/N cells were transfected with 1000 ng pL enti6.3/V5‐ DEST ‐slug, and 1000 ng pGL ‐Basic‐ephrin‐B1 promoter WT , E1m, E2m, or E1mE2m. F, Ch IP analysis of slug on the E‐box regions in the ephrin‐B1 promoter. Soluble chromatin extracted from LNC aP/ CH 6M cells was immunoprecipitated with anti‐slug and control IgG antibodies. Ch IP was analyzed on quantitative PCR using primers flanking the E‐box motifs, E‐box1 and E‐box2, shown in (C). Fold change compared with control IgG‐enriched DNA fragments was measured. Data given as mean ± SD . * P

    Journal: Cancer Science

    Article Title: Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1, et al. Chronic hypoxia‐induced slug promotes invasive behavior of prostate cancer cells by activating expression of ephrin‐B1

    doi: 10.1111/cas.13754

    Figure Lengend Snippet: Chronic hypoxia‐induced slug activates expression of ephrin‐B1 through E‐box motifs. A, Relative expression of ephrin‐B1 mRNA in non‐transfected (control), slug si RNA ‐transfected (siSlug) and control si RNA ‐transfected (siScr) LNC aP/ CH 6M cells. B, Relative expression of ephrin‐B1 mRNA in the LNC aP/N cells transfected with various amounts of the slug‐expression vector, pL enti6.3/V5‐ DEST ‐slug (0‐1000 ng). C, Schematic diagram of luciferase reporter constructs containing wild‐type human ephrin‐B1 promoter ( pGL ‐Basic‐ephrin‐B1 promoter WT ), E‐box1 (E1m) or E‐box2 (E2m) mutations, or double mutations (E1mE2m). Arrows, primer pairs flanking E‐box motifs used for chromatin immunoprecipitation (ChIP) analysis. D, Effect of slug overexpression on the wild‐type ephrin‐B1 promoter activity. LNC aP/N cells were transfected with 1000 ng pGL ‐Basic‐ephrin‐B1 promoter WT and various amounts of the slug‐expression vector, pL enti6.3/V5‐ DEST ‐slug (0‐1000 ng). Firefly luciferase activity was normalized to Renilla luciferase activity. E, Mutational analysis of the E‐box motifs in the ephrin‐B1 promoter. LNC aP/N cells were transfected with 1000 ng pL enti6.3/V5‐ DEST ‐slug, and 1000 ng pGL ‐Basic‐ephrin‐B1 promoter WT , E1m, E2m, or E1mE2m. F, Ch IP analysis of slug on the E‐box regions in the ephrin‐B1 promoter. Soluble chromatin extracted from LNC aP/ CH 6M cells was immunoprecipitated with anti‐slug and control IgG antibodies. Ch IP was analyzed on quantitative PCR using primers flanking the E‐box motifs, E‐box1 and E‐box2, shown in (C). Fold change compared with control IgG‐enriched DNA fragments was measured. Data given as mean ± SD . * P

    Article Snippet: 2.1 Plasmids and mutagenesis pLenti6.3/V5‐DEST‐ephrin‐B1 and ‐slug were created by recombining the Gateway entry vectors containing the human ephrin‐B1 cDNA (FLJ80318AAAF, NITE Biological Research Center, Chiba, Japan) and slug cDNA (FLJ93051AAAF) with the destination vector pLenti6.3/V5‐DEST (Invitrogen, Carlsbad, CA, USA).

    Techniques: Expressing, Transfection, Plasmid Preparation, Luciferase, Construct, Chromatin Immunoprecipitation, Over Expression, Activity Assay, Immunoprecipitation, Real-time Polymerase Chain Reaction

    Schematic diagram of construction of IL-10/Lentivirus. IL-10/lentivirus-based plasmid was made by inserting the mIL-10 cDNA into the pLenti6.3/V5-TOPO vector.

    Journal: PLoS ONE

    Article Title: Magnetic Resonance Imaging of Bone Marrow Cell-Mediated Interleukin-10 Gene Therapy of Atherosclerosis

    doi: 10.1371/journal.pone.0024529

    Figure Lengend Snippet: Schematic diagram of construction of IL-10/Lentivirus. IL-10/lentivirus-based plasmid was made by inserting the mIL-10 cDNA into the pLenti6.3/V5-TOPO vector.

    Article Snippet: MIL-10-lentivirus production A lentivirus-based plasmid was made by inserting the mIL-10 cDNA into the pLenti6.3/V5-TOPO vector (Invitrogen, Carlsbad, CA, USA) ( ).

    Techniques: Plasmid Preparation