Journal: International Journal of Molecular Sciences

Article Title: A Medium-Throughput System for In Vitro Oxidative Stress Assessment in IPEC-J2 Cells

doi: 10.3390/ijms21197263

Figure Lengend Snippet: Autofluorescence of the three tested cell culture plates, differing in the material used in the bottom (plastic: polystyrene cell culture microplate Clear ® (Greiner Bio-One, Vilvoorde, Belgium); film: cell imaging microplate 25-μm film bottom (Eppendorf, Aarschot, Belgium) and glass: cell imaging microplate 170-μm coverglass bottom (Eppendorf)). Fluorescence intensity is represented in arbitrary units (AU). Plastic-bottomed plates show the highest autofluorescence values, while glass-bottomed plates showed the lowest. Film-bottomed plates showed intermediate autofluorescence that statistically differed from the two former plate types. Different letters denote values that are significantly different.

Article Snippet: Three 96-well plates were considered: polystyrene cell culture microplate μClear ® (Greiner Bio-One, Volvoorde, Belgium; Cat No 655079) (Plastic), cell imaging microplate 25-μm film bottom (Eppendorf, Aarschot, Belgium Cat No 0030741013) (Film) and cell imaging microplate 170-μm coverglass bottom (Eppendorf, Aarschot, Belgium; Cat No 0030741021) (Glass).

Techniques: Cell Culture, Imaging, Fluorescence

Journal: medRxiv

Article Title: Bio-Rad and QIAGEN digital PCR platforms provide equivalent quantification for wastewater-based SARS-CoV-2 surveillance

doi: 10.64898/2026.01.20.26344437

Figure Lengend Snippet: Quantitative comparison of SARS-CoV-2 targets between Bio-Rad QX200 ddPCR and QIAGEN QIAcuity dPCR platforms across all concentration ranges. Scatter plots showing hyperwelled (merged triplicate) concentrations for (A) N1 gene target and (B) N2 gene target. Each point represents a single wastewater sample (n = 95) plotted as log 10 copies L -1 on both axes. Samples are color-coded by concentration bin: high (red, 5×10 4 to 5×10 5 copies L -1 , n=31), medium (orange, 5×10 3 to 5×10 4 copies L -1 , n=32), and low (blue, 1×10 3 to 5×10 3 copies L -1 , n=31). Solid black line represents perfect 1:1 agreement; dashed line showed linear regression fit. Linear regression equations, R 2 values, and 95% confidence intervals (gray shading) are displayed for each target. Mean absolute differences between platforms: N1 = 0.10 log copies L -1 , N2 = 0.06 log copies L -1 . All correlations significant at p < 0.001.

Article Snippet: Total nucleic acids were eluted in 100μL of Buffer AE (19077, QIAGEN, Germantown, MD) and transferred into 96-Well ddPCR Plates (12001925, Bio-Rad Laboratories).

Techniques: Comparison, Concentration Assay